LOCUS       V016113                 7664 bp    DNA     circular SYN 01-FEB-2022
DEFINITION  Exported.
ACCESSION   V016113
VERSION     V016113
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ruf S, Forner J, Hasse C, Kroop X, Seeger S, Schollbach L, Schadach
            A, Bock R.
  TITLE     High-efficiency generation of fertile transplastomic Arabidopsis
            plants
  JOURNAL   Nat Plants 5 (3), 282-289 (2019)
   PUBMED   30778165
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Forner J, Hasse C, Ruf S, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2018) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany
REFERENCE   3  (bases 1 to 7664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat
            Plants"; date: "2019"; volume: "5"; issue: "3"; pages: "282-289"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUL-2018) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany"
FEATURES             Location/Qualifiers
     source          1..7664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..521
                     /gene="psaB"
                     /label="psaB"
     CDS             1..521
                     /codon_start=3
                     /transl_table=11
                     /gene="psaB"
                     /product="PSAB"
                     /label="psaB"
                     /protein_id="QBG49744.1"
                     /translation="GRGGTCDISAWDAFYLAVFWMLNTIGWVTFYWHWKHITLWQGNVS
                     QFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLVWATGFMFLIS
                     WRGYWQELIETLAWAHERTPLANLIRWKDKPVALSIVQARLVGLAHFSVGYIFTYAAFL
                     IASTSGKFG"
     CDS             656..955
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from
                     Arabidopsis thaliana. Accession#: P56804"
     tRNA            1119..1192
                     /product="tRNA-Met"
                     /label="trnfM"
                     /note="trnfM"
     regulatory      1302..1451
                     /label="Zea mays clpP promoter"
                     /note="Zea mays clpP promoter"
                     /regulatory_class="promoter"
     CDS             1453..2241
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      2252..2338
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2430..2457
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      2475..2709
                     /label="Chlamydomonas reinhardtii psaA promoter"
                     /note="Chlamydomonas reinhardtii psaA promoter"
                     /regulatory_class="promoter"
     CDS             2711..3427
                     /label="Venus"
                     /note="yellow fluorescent protein (YFP) with fast and
                     efficient maturation (Nagai et al., 2002)"
     regulatory      3431..3603
                     /label="Chlamydomonas reinhardtii atpB terminator"
                     /note="Chlamydomonas reinhardtii atpB terminator"
                     /regulatory_class="terminator"
     tRNA            complement(3667..3737)
                     /product="tRNA-Gly"
     CDS             complement(4291..4476)
                     /gene="psbZ"
                     /label="Photosystem II reaction center protein Z"
                     /note="Photosystem II reaction center protein Z from
                     Nicotiana tabacum. Accession#: P09974"
     promoter        complement(4815..4833)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4843..4859)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5001..5456
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5482..5586
                     /label="AmpR promoter"
     CDS             5587..6444
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6618..7206
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7494..7515
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7530..7560
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7568..7584
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7592..7608
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7629..7647
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgggacgagg tggtacttgt gatatttcgg cttgggacgc attttatttg gcagttttct
       61 ggatgttaaa tactattgga tgggttactt tttattggca ttggaaacac atcacattat
      121 ggcagggtaa cgtttcacag tttaatgaat cttccactta tttgatggga tggttaagag
      181 attatctatg gttaaactct tcacaactta tcaatggata taacccgttt ggtatgaata
      241 gtttatcagt ctgggcatgg atgttcttat ttgggcatct tgtttgggct acaggattta
      301 tgttcttaat ttcctggcgt ggttattggc aggaattgat tgaaacttta gcgtgggctc
      361 atgaacgcac acctttggca aatttgattc gctggaaaga taaaccagta gctctttcaa
      421 tcgtgcaagc aagattggtt ggattagctc acttttctgt aggttatata ttcacttatg
      481 cggctttctt gattgcctcc acatcgggca aattcggtta attatttatg tattctatcc
      541 gcaatcatat atttttttta ataaaaaaaa tataggtatg cactcttata tttatttcta
      601 catctaggat ccgatttgta tcattatcat tgataataac aggaactgaa gcattatggc
      661 aaagaaaagt ttgatttata gggagaagaa gaggcaaaaa ttggaaaaaa aatatcattt
      721 gattcgtcga tcctcaaaaa aggaaataag taagattccg tcgctaagtg agaaatggaa
      781 aattcatgga aaattacaat ccccaccgcg taatagtgca cctacacgtc ttcatcgacg
      841 ttgcttttcg accggaagac cgagagctaa ctatcgagac tttggactat ctggacacat
      901 ccttcgggaa atggttcagg catgtttgtt gccaggggca acaagatcaa gctggtaagg
      961 atttaagtat cccttaattt ttctattttt ttctatgatc gatgaggccc ctttaccatt
     1021 ctgtctaaat aggctattct atttgtacag atatggaaga ggggctcctt caattattct
     1081 ttgtttatta gagtttaatc caagtttttt tgtttcagcg cggggtagag cagtttggta
     1141 gctcgcaagg ctcataacct tgaggtcacg ggttcaaatc ctgtctccgc aacttttttt
     1201 tttcaagaaa tgtaagtttg attctactaa caagtcataa attaatacgt gtcttttggg
     1261 acgcgaggaa aaaattacaa tatttcccgg agctcggatc ctctatgtat taatagaatc
     1321 tatagtattc ttatagaata agaaaaaaaa aatgaagata ataaactgcg gattctttct
     1381 ttctcttcca ttcttacgtt tccatattaa agtgtagttt ttttacttaa atttaaagaa
     1441 ggagatatac ccatggaaga agctgttatt gctgaagttt ctacacaatt atctgaagtt
     1501 gttggagtta ttgaaagaca tttagaacct acattattag ctgttcattt atatggatct
     1561 gctgttgatg gaggattaaa acctcattct gatattgatt tattagttac tgttacagtt
     1621 agattagatg aaactacaag aagagcttta attaatgatt tattagaaac ttctgcttct
     1681 cctggagaat ctgaaatttt aagagctgtt gaagttacta ttgttgttca tgatgatatt
     1741 attccttgga gatatcctgc taaaagagaa ttacaatttg gagaatggca aagaaatgat
     1801 attttagctg gaatttttga acctgctact attgatattg atttagctat tttattaaca
     1861 aaagctagag aacattctgt tgctttagtt ggacctgctg ctgaagaatt atttgatcct
     1921 gttccggaac aagatttatt tgaagcatta aatgaaacat taactttatg gaactctcct
     1981 cctgattggg ctggagatga aagaaatgtt gttttaacat tatccagaat ttggtattct
     2041 gctgttacag gaaaaattgc tcctaaagat gttgctgctg attgggctat ggaaagatta
     2101 cctgctcaat atcaacctgt tattttagaa gctagacagg cttatttagg acaagaagaa
     2161 gatagattag cttcaagagc tgatcaatta gaagaatttg ttcattatgt taaaggagaa
     2221 attacaaaag ttgttggaaa ataatctaga tcaaataaaa cgaaaggctc agtcgaaaga
     2281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2341 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     2401 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     2461 gtttctacaa gcttttttac gaatacacat atggtaaaaa ataaaacaat atctttaaaa
     2521 taagtaaaaa taatttgtaa accaataaaa aatatattta tggtataata taacatatga
     2581 tgtaaaaaaa actatttgtc taatttaata accatgcatt ttttatgaac acataataat
     2641 taaaagcgtt gctaatggtg taaataatgt atttattaaa ttaaataatt gttattataa
     2701 ggagaaatca atggtttcaa aaggtgaaga attatttaca ggtgttgttc caattttagt
     2761 tgaattagat ggtgatgtaa atggtcacaa attttcagta tcaggtgaag gtgaaggtga
     2821 tgctacatat ggtaaattaa cattaaaatt aatttgtaca acaggtaaat taccagttcc
     2881 ttggccaaca ttagttacaa cattaggtta tggtttacaa tgttttgctc gttatccaga
     2941 tcatatgaaa caacatgatt tttttaaatc agctatgcca gaaggttatg ttcaagaacg
     3001 tacaattttt tttaaagatg atggtaatta taaaacacgt gctgaagtta aatttgaagg
     3061 tgatacatta gtaaatcgta ttgaattaaa aggtattgat tttaaagaag atggtaatat
     3121 tttaggtcac aaattagaat ataattataa ttcacacaat gtatatatta cagctgataa
     3181 acaaaaaaat ggtattaaag ctaattttaa aattcgtcat aatattgaag atggtggtgt
     3241 tcaattagct gatcactatc aacaaaatac accaattggt gatggtccag tattattacc
     3301 agataatcat tatttatcat atcaatcagc tttatcaaaa gatccaaatg aaaaacgtga
     3361 tcatatggta ttattagaat ttgtaacagc tgctggtatt acattaggta tggatgaatt
     3421 aattaaatga tttaattttt tatgtgtaat taaaataaat atttggacac cattaagttg
     3481 ttttcttctt aaagagccaa tttattttaa ttacacatac attatatatg tcatattagc
     3541 tatttaatct atttacttta aaataaagga cgccgttaaa cgtgtattta ataatgtgtt
     3601 aaactcgagg gggggcccgg tacaactata ccgaatattt tctctttttg aatccattta
     3661 tatttgggcg gatagcggga atcgaacccg cgtcttctcc ttggcaagga gaaattttac
     3721 cattcgacta tatccgcatt ttttgccctt ctttataaga aatttatgga taatatttgt
     3781 tcaaagctag acgagataca ctctttggtt tggggtcatt tcataaaatt ctaccaccaa
     3841 ataaattcaa ttaacaattc tattaatata tatatatata ttataaatat atatattaat
     3901 aatatatatt atatttattc tatatattaa tatattaata ttaattcttg aatattaatt
     3961 taatttaata ttgaattcca gattcaagaa tatattattc ttatatttcc ataaaatatt
     4021 atattctata ttgacttgat atcagatctc aattttttat tcgttttttt tatttatttt
     4081 tttttattac tatttcatat ttaggaacta tttcatattt agtaactatt tattaatctt
     4141 ttattcatat ttcattcaag ttccataagt tcgacccccc ctctaatttt ttctttattt
     4201 gcattttatg gacttatatt gaatttgaat gtgtaattca aggaatggga gggggtcatc
     4261 tcatttttgg atctgcaacg aatgaattca agagataaga gaattaagga tacccaccaa
     4321 gaagactaat ccaatccata aagatgtacc agaaaataca acatttttgt tactcgacca
     4381 accatcagga gacgcaaata caacgggtac actaatcagt aagattgatg aagtaataat
     4441 taatgcaaaa actgccaatt ggaaagcaat agtcatgttt ttaatcctcc aagctattaa
     4501 caaaagaata tacaccattt aatcctctta cccactaaaa atttttaata aataaaggga
     4561 ttttatcatg aatccgttga ttcgagatga cttagttcca atttcttttt accactttta
     4621 gtctattcgg acatgaagtt aaaggttctt ttttacttca caaacgggta tactggatcg
     4681 cgtatctcat ttatttatat agccagattt atagacctat taaagaaagt cacaccctat
     4741 atctttctct acatagtgat cgtattaact catagggcta tgttctattt ggcgaaggta
     4801 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     4861 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     4921 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     4981 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     5041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     5101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     5161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     5221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     5281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     5341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     5401 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     5461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5821 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     6001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     6061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     6121 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     6181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     6241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     6301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     6361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6961 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7021 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7081 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7141 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7201 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7261 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     7321 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     7381 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7441 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     7501 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     7561 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     7621 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agat
//
