LOCUS       V015834                 7661 bp    DNA     circular SYN 03-MAR-2019
DEFINITION  Exported.
ACCESSION   V015834
VERSION     V015834
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ruf S, Forner J, Hasse C, Kroop X, Seeger S, Schollbach L, Schadach
            A, Bock R.
  TITLE     High-efficiency generation of fertile transplastomic Arabidopsis
            plants
  JOURNAL   Nat Plants (2019) In press
   PUBMED   30778165
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Forner J, Hasse C, Ruf S, Bock R.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JUL-2018) Organelle Biology, Biotechnology and
            Molecular Ecophysiology, Max Planck Institute of Molecular Plant
            Physiology, Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany
REFERENCE   3  (bases 1 to 7661)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants
            (2019) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-JUL-2018) Organelle Biology, Biotechnology and Molecular
            Ecophysiology, Max Planck Institute of Molecular Plant Physiology,
            Am Muehlenberg 1, Potsdam, Brandenburg D-14476, Germany"
FEATURES             Location/Qualifiers
     source          1..7661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1..521
                     /gene="psaB"
                     /label="psaB"
     CDS             1..521
                     /codon_start=3
                     /transl_table=11
                     /gene="psaB"
                     /product="PSAB"
                     /label="psaB"
                     /protein_id="QBG49738.1"
                     /translation="GRGGTCDISAWDAFYLAVFWMLNTIGWVTFYWHWKHITLWQGNVS
                     QFNESSTYLMGWLRDYLWLNSSQLINGYNPFGMNSLSVWAWMFLFGHLVWATGFMFLIS
                     WRGYWQELIETLAWAHERTPLANLIRWKDKPVALSIVQARLVGLAHFSVGYIFTYAAFL
                     IASTSGKFG"
     CDS             656..955
                     /gene="rps14"
                     /label="Small ribosomal subunit protein uS14c"
                     /note="Small ribosomal subunit protein uS14c from
                     Arabidopsis thaliana. Accession#: P56804"
     tRNA            1119..1192
                     /product="tRNA-Met"
                     /label="trnfM"
                     /note="trnfM"
     regulatory      1302..1451
                     /label="Zea mays clpP promoter"
                     /note="Zea mays clpP promoter"
                     /regulatory_class="promoter"
     CDS             1453..2241
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     terminator      2252..2338
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2430..2457
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     regulatory      2475..2709
                     /label="Chlamydomonas reinhardtii psaA promoter"
                     /note="Chlamydomonas reinhardtii psaA promoter"
                     /regulatory_class="promoter"
     CDS             2711..3424
                     /label="mgfp5"
                     /note="GFP with folding enhancement mutations"
     regulatory      3428..3600
                     /label="Chlamydomonas reinhardtii atpB terminator"
                     /note="Chlamydomonas reinhardtii atpB terminator"
                     /regulatory_class="terminator"
     tRNA            complement(3664..3734)
                     /product="tRNA-Gly"
     CDS             complement(4288..4473)
                     /gene="psbZ"
                     /label="Photosystem II reaction center protein Z"
                     /note="Photosystem II reaction center protein Z from
                     Nicotiana tabacum. Accession#: P09974"
     promoter        complement(4812..4830)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4840..4856)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4998..5453
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5479..5583
                     /label="AmpR promoter"
     CDS             5584..6441
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6615..7203
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    7491..7512
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7527..7557
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    7565..7581
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7589..7605
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7626..7644
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgggacgagg tggtacttgt gatatttcgg cttgggacgc attttatttg gcagttttct
       61 ggatgttaaa tactattgga tgggttactt tttattggca ttggaaacac atcacattat
      121 ggcagggtaa cgtttcacag tttaatgaat cttccactta tttgatggga tggttaagag
      181 attatctatg gttaaactct tcacaactta tcaatggata taacccgttt ggtatgaata
      241 gtttatcagt ctgggcatgg atgttcttat ttgggcatct tgtttgggct acaggattta
      301 tgttcttaat ttcctggcgt ggttattggc aggaattgat tgaaacttta gcgtgggctc
      361 atgaacgcac acctttggca aatttgattc gctggaaaga taaaccagta gctctttcaa
      421 tcgtgcaagc aagattggtt ggattagctc acttttctgt aggttatata ttcacttatg
      481 cggctttctt gattgcctcc acatcgggca aattcggtta attatttatg tattctatcc
      541 gcaatcatat atttttttta ataaaaaaaa tataggtatg cactcttata tttatttcta
      601 catctaggat ccgatttgta tcattatcat tgataataac aggaactgaa gcattatggc
      661 aaagaaaagt ttgatttata gggagaagaa gaggcaaaaa ttggaaaaaa aatatcattt
      721 gattcgtcga tcctcaaaaa aggaaataag taagattccg tcgctaagtg agaaatggaa
      781 aattcatgga aaattacaat ccccaccgcg taatagtgca cctacacgtc ttcatcgacg
      841 ttgcttttcg accggaagac cgagagctaa ctatcgagac tttggactat ctggacacat
      901 ccttcgggaa atggttcagg catgtttgtt gccaggggca acaagatcaa gctggtaagg
      961 atttaagtat cccttaattt ttctattttt ttctatgatc gatgaggccc ctttaccatt
     1021 ctgtctaaat aggctattct atttgtacag atatggaaga ggggctcctt caattattct
     1081 ttgtttatta gagtttaatc caagtttttt tgtttcagcg cggggtagag cagtttggta
     1141 gctcgcaagg ctcataacct tgaggtcacg ggttcaaatc ctgtctccgc aacttttttt
     1201 tttcaagaaa tgtaagtttg attctactaa caagtcataa attaatacgt gtcttttggg
     1261 acgcgaggaa aaaattacaa tatttcccgg agctcggatc ctctatgtat taatagaatc
     1321 tatagtattc ttatagaata agaaaaaaaa aatgaagata ataaactgcg gattctttct
     1381 ttctcttcca ttcttacgtt tccatattaa agtgtagttt ttttacttaa atttaaagaa
     1441 ggagatatac ccatggaaga agctgttatt gctgaagttt ctacacaatt atctgaagtt
     1501 gttggagtta ttgaaagaca tttagaacct acattattag ctgttcattt atatggatct
     1561 gctgttgatg gaggattaaa acctcattct gatattgatt tattagttac tgttacagtt
     1621 agattagatg aaactacaag aagagcttta attaatgatt tattagaaac ttctgcttct
     1681 cctggagaat ctgaaatttt aagagctgtt gaagttacta ttgttgttca tgatgatatt
     1741 attccttgga gatatcctgc taaaagagaa ttacaatttg gagaatggca aagaaatgat
     1801 attttagctg gaatttttga acctgctact attgatattg atttagctat tttattaaca
     1861 aaagctagag aacattctgt tgctttagtt ggacctgctg ctgaagaatt atttgatcct
     1921 gttccggaac aagatttatt tgaagcatta aatgaaacat taactttatg gaactctcct
     1981 cctgattggg ctggagatga aagaaatgtt gttttaacat tatccagaat ttggtattct
     2041 gctgttacag gaaaaattgc tcctaaagat gttgctgctg attgggctat ggaaagatta
     2101 cctgctcaat atcaacctgt tattttagaa gctagacagg cttatttagg acaagaagaa
     2161 gatagattag cttcaagagc tgatcaatta gaagaatttg ttcattatgt taaaggagaa
     2221 attacaaaag ttgttggaaa ataatctaga tcaaataaaa cgaaaggctc agtcgaaaga
     2281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
     2341 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
     2401 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
     2461 gtttctacaa gcttttttac gaatacacat atggtaaaaa ataaaacaat atctttaaaa
     2521 taagtaaaaa taatttgtaa accaataaaa aatatattta tggtataata taacatatga
     2581 tgtaaaaaaa actatttgtc taatttaata accatgcatt ttttatgaac acataataat
     2641 taaaagcgtt gctaatggtg taaataatgt atttattaaa ttaaataatt gttattataa
     2701 ggagaaatca atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     2761 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2821 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     2881 gccaacactt gtcactactt tgtgttatgg tgttcaatgc ttttcaagat acccagatca
     2941 catgaagcgg cacgacttct tcaagagcgc catgcctgag ggatacgtgc aggagaggac
     3001 catcttcttc aaggacgacg ggaactacaa gacacgtgct gaagtcaagt ttgagggaga
     3061 caccctcgtc aacaggatcg agctaaaggg aatcgatttc aaggaggacg gaaacatcct
     3121 cggccacaag ttggaataca actacaactc ccacaacgta tacatcatgg ccgacaagca
     3181 aaagaacggc atcaaagcca acttcaagac ccgccacaac atcgaagatg gcggcgtgca
     3241 actcgctgat cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     3301 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagatca
     3361 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     3421 taaatgattt aattttttat gtgtaattaa aataaatatt tggacaccat taagttgttt
     3481 tcttcttaaa gagccaattt attttaatta cacatacatt atatatgtca tattagctat
     3541 ttaatctatt tactttaaaa taaaggacgc cgttaaacgt gtatttaata atgtgttaaa
     3601 ctcgaggggg ggcccggtac aactataccg aatattttct ctttttgaat ccatttatat
     3661 ttgggcggat agcgggaatc gaacccgcgt cttctccttg gcaaggagaa attttaccat
     3721 tcgactatat ccgcattttt tgcccttctt tataagaaat ttatggataa tatttgttca
     3781 aagctagacg agatacactc tttggtttgg ggtcatttca taaaattcta ccaccaaata
     3841 aattcaatta acaattctat taatatatat atatatatta taaatatata tattaataat
     3901 atatattata tttattctat atattaatat attaatatta attcttgaat attaatttaa
     3961 tttaatattg aattccagat tcaagaatat attattctta tatttccata aaatattata
     4021 ttctatattg acttgatatc agatctcaat tttttattcg ttttttttat ttattttttt
     4081 ttattactat ttcatattta ggaactattt catatttagt aactatttat taatctttta
     4141 ttcatatttc attcaagttc cataagttcg accccccctc taattttttc tttatttgca
     4201 ttttatggac ttatattgaa tttgaatgtg taattcaagg aatgggaggg ggtcatctca
     4261 tttttggatc tgcaacgaat gaattcaaga gataagagaa ttaaggatac ccaccaagaa
     4321 gactaatcca atccataaag atgtaccaga aaatacaaca tttttgttac tcgaccaacc
     4381 atcaggagac gcaaatacaa cgggtacact aatcagtaag attgatgaag taataattaa
     4441 tgcaaaaact gccaattgga aagcaatagt catgttttta atcctccaag ctattaacaa
     4501 aagaatatac accatttaat cctcttaccc actaaaaatt tttaataaat aaagggattt
     4561 tatcatgaat ccgttgattc gagatgactt agttccaatt tctttttacc acttttagtc
     4621 tattcggaca tgaagttaaa ggttcttttt tacttcacaa acgggtatac tggatcgcgt
     4681 atctcattta tttatatagc cagatttata gacctattaa agaaagtcac accctatatc
     4741 tttctctaca tagtgatcgt attaactcat agggctatgt tctatttggc gaaggtacgt
     4801 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4861 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4921 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4981 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5041 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     5101 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5161 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5221 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5281 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5341 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5401 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     5461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5821 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     6001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     6061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     6121 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     6181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     6241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     6301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     6361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     6421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6601 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6661 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6721 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     6781 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6841 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6901 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6961 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7021 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7081 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7141 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7201 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     7261 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     7321 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     7381 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     7441 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7501 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7561 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     7621 cgcgcaatta accctcacta aagggaacaa aagctggaga t
//
