LOCUS       40924_26016        3128 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Positive selection cloning vector pJET1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Positive selection cloning vector pJET1
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Lubys A, Pagarauskaite K.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2005) R
REFERENCE   3  (bases 1 to 3128)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3128)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2005) R"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(187..879)
                     /codon_start=1
                     /gene="eco47IR"
                     /product="restriction endonuclease Eco47I"
                     /label=eco47IR
                     /note="restriction endonuclease; in the absence of
                     protective methylation the enzyme recognizes and cleaves 
                     double-stranded DNA targets 5'-GGWCC-3'"
                     /protein_id="ABC47403.1"
                     /translation="MSKETSFVKNAEELAKQKMDAINPELSSKFKFLIKFLSQFPEACS
                     KPRSKKMQNKVGQEEHIEYLARSFHESRLPRKPTPPTTVPDEVVSIVLNISFNIQPENL
                     ERIKEEHRFSMAAENIVGDLLERYLAEKLEPSGWIWCSGTSVKAVDFIHYDEKNNEWNL
                     LQVKNRDNTENSSSSKIRDNTTIKKWFRTYSQRDATNWDNFPDEVSSKNLNEEDFRAFV
                     KNYLAKII"
     gene            complement(187..879)
                     /gene="eco47IR"
                     /label=eco47IR
     protein_bind    complement(908..924)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(932..962)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(977..998)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1309..1897)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2071..2928)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2929..3033)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaagctag catgcggccg cgagctcgtc gacacgtgaa
      181 ttcgaattat attatttttg ccaaataatt tttaacaaaa gctctgaagt cttcttcatt
      241 taaattctta gatgatactt catctggaaa attgtcccaa ttagtagcat cacgctgtga
      301 gtaagttcta aaccattttt ttattgttgt attatctcta atcttactac tcgatgagtt
      361 ttcggtatta tctctatttt taacttggag caggttccat tcattgtttt tttcatcata
      421 gtgaataaaa tcaactgctt taacacttgt gcctgaacac catatccatc cggatggctc
      481 gagtttttca gcaagatatc tttctagaag atctcctaca atattctcag ctgccatgga
      541 aaatcgatgt tcttctttta ttctctcaag attttcaggc tgtatattaa aacttatatt
      601 aagaactatg ctaaccacct catcaggaac cgttgtaggt ggcgtgggtt ttcttggcaa
      661 tcgactctca tgaaaactac gagctaaata ttcaatatgt tcctcttgac caactttatt
      721 ctgcattttt tttgaacgag gtttagagca agcttcagga aactgagaca ggaattttat
      781 taaaaattta aattttgaag aaagttcagg gttaatagca tccatttttt gctttgcaag
      841 ttcctcagca ttcttaacaa aagacgtctc ttttgacatg tttaaagttt aaacctcctg
      901 tgtgaaattg ttatccgctc acaattccac acattatacg agccggaagc ataaagtgta
      961 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgccaa
     1021 ttggatcccg ggcccctgca gcgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     1081 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     1141 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     1201 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1261 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1321 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1381 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1441 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1501 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1561 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     1621 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     1681 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     1741 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     1801 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     1861 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     1921 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     1981 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     2041 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     2101 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     2161 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     2221 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     2281 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     2341 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     2401 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     2461 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     2521 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     2581 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     2641 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     2701 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     2761 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     2821 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     2881 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     2941 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     3001 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     3061 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     3121 ctttcgtc
//
