LOCUS       Exported                3005 bp DNA     circular SYN 03-SEP-2024
DEFINITION  Positive selection cloning vector with a lethal insert that allows 
            for efficient recovery of blunt-ended PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pJET1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Thermo Scientific (Fermentas)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3005)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3005)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize at Eco32I (EcoRV) to insert a blunt PCR product.
FEATURES             Location/Qualifiers
     source          1..3005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          372..402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    328..453
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site with a central Eco32I (EcoRV)
                     site"
     protein_bind    complement(813..829)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(837..867)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(882..903)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1194..1782)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1956..2813)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2814..2918)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatctcatcc tacgtttaca agtcttaaag atatctttct agaagatctc
      421 ctacaatatt ctcagctgcc atggaaaatc gatgttcttc ttttattctc tcaagatttt
      481 caggctgtat attaaaactt atattaagaa ctatgctaac cacctcatca ggaaccgttg
      541 taggtggcgt gggttttctt ggcaatcgac tctcatgaaa actacgagct aaatattcaa
      601 tatgttcctc ttgaccaact ttattctgca ttttttttga acgaggttta gagcaagctt
      661 caggaaactg agacaggaat tttattaaaa atttaaattt tgaagaaagt tcagggttaa
      721 tagcatccat tttttgcttt gcaagttcct cagcattctt aacaaaagac gtctcttttg
      781 acatgtttaa agtttaaacc tcctgtgtga aattattatc cgctcataat tccacacatt
      841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      901 ttaattgcgt tgcgctcact gccaattgct ttccagtcgg gaaacctgtc gtgccagctg
      961 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     1021 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     1081 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     1141 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1201 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1261 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1321 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1381 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1441 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1501 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1561 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1621 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1681 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1741 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1801 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1861 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1921 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1981 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2041 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2101 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2161 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2221 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2281 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2341 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2401 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2461 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2521 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2581 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2641 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2701 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2761 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2821 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2881 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     2941 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     3001 aggcc
//
