LOCUS       62056_13115        2988 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(799..815)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(823..853)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(868..889)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1177..1765)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1939..2796)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2797..2901)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatcgactag accaggatat ctttctagaa gatctcctac aatattctca
      421 gctgccatgg aaaatcgatg ttcttctttt attctctcaa gattttcagg ctgtatatta
      481 aaacttatat taagaactat gctaaccacc tcatcaggaa ccgttgtagg tggcgtgggt
      541 tttcttggca atcgactctc atgaaaacta cgagctaaat attcaatatg ttcctcttga
      601 ccaactttat tctgcatttt ttttgaacga ggtttagagc aagcttcagg aaactgagac
      661 aggaatttta ttaaaaattt aaattttgaa gaaagttcag ggttaatagc atccattttt
      721 tgctttgcaa gttcctcagc attcttaaca aaagacgtct cttttgacat gtttaaagtt
      781 taaacctcct gtgtgaaatt gttatccgct cacaattcca cacattatac gagccggaag
      841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     1381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     1621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     1681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     1741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     1801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     1861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     1921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     1981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     2101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     2161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     2221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     2281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     2341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     2401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     2461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     2521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     2581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     2641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     2701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     2761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     2821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     2881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     2941 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcc
//
