LOCUS       .                       4887 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        305..323
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(2698..2714)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2722..2752)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(2767..2788)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3076..3664)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3838..4695)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(4696..4800)
                     /label="AmpR promoter"
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagatg
      361 gaagccgtgc tcaatagtga tgggttttta acatctaacg caggactaaa atctagttat
      421 attaatcaaa ctctttcttt ggtagatgaa gatcatatac acacttctga taaatctttg
      481 tcttgtagtg tatgcaattc attatcccag attgtagacg atgactttat atccgcagga
      541 gctagaaatc aacgtaccaa acctaaacgt gcaggaaata atcaatctca acagcctatc
      601 aaaaaggatt gtatggtttc catcgacgaa gtagcatcca cgcatgattg gagtacgaga
      661 ttgagaaatg atgggaatgc aattgctaaa tatctaacta ctaacaagta tgacacatct
      721 aactttacta ttcaggatat gcttaacatt atgaataaac taaatattgt cagaacaaat
      781 agaaacgagc tatttcaact ccttacccat gtaaagagca cgttgaacaa tgctagtgtt
      841 tctgtgaaat gtactcatcc tttagtactt attcattctc gagctagtcc tagaatcggt
      901 gaccaactca aagagttaga taaaatatac tctccatcta atcatcatat tcttctgtcg
      961 actacacgat tccaatccat gcattttacc gatatgtcta gttcacaaga tttgtctttt
     1021 atttatagaa aaccagaaac taattactac attcatccta ttctgatggc actattcggt
     1081 attaaacttc cagcgctcga gaacgcgtat gtacatggag acacatatag tctaatccag
     1141 caactttatg aatttagaag agtaaagtct tataattata tgttgttggt taatcgtctt
     1201 acggaggata atccgatagt gattacaggt gtatcagatc taatttccac agagattcag
     1261 agagcaaaca tgcataccat gattagaaaa gcaattatga acattagaat gggaattttt
     1321 tattgtaacg atgatgatgc ggtagatccc catctaatga agattattca tactggatgc
     1381 tctcaagtta tgaccgatga ggaacagata ttagcttcta ttttgtctat agttggattt
     1441 agacctacgt tggtttctgt ggctagacct atgaacggca tcagttacga tatgaaactt
     1501 caggcggcgc catacatcgt tgttaatcct atgaagatga tcacaacatc cgacagtccg
     1561 atttctatca attccaagga tatttattct atggcattcg atggcaatag tggaagagtg
     1621 gtgttcgctc ctcctaacat aggctatgga agatgttctg gagttacaca cattgatcca
     1681 ttgggaacta atgtgatggg tagtgctgtt cattcccctg ttatcgttaa tggagcaatg
     1741 atgttttatg tagaacgacg ccagaataag aatatgtttg gtggagaatg ttacaccggc
     1801 tttagatctc taatagatga tactccaatt gacgtatcgc cagaaatcat gctaaacggt
     1861 atcatgtata ggttaaagtc cgcagtctgt tataaactcg gagaccaatt ctttgattgt
     1921 ggatcgtctg atatcttctt gaagggacat tatacgattc tatttacaga aaatggaccc
     1981 tggatgtacg atcctctttc tgttttcaat ccgggagcta gaaatgctag attgatgaga
     2041 gctctcaaaa accagtacaa gaaattatca atggattcag acgatggttt ttatgaatgg
     2101 ttgaatggcg atggttcagt atttgctgcc tcaaaacagc aaatgttgat gaatcacgtt
     2161 gctaactttg acgacgatct tctaactatg gaagaagcca tgtcgatgat ttcaagacat
     2221 tgttgtatct taatttatgc acaggattat gatcaatata ttagcgctag gcatattaca
     2281 gaactatttt agtctagaag atctcctaca atattctcag ctgccatgga aaatcgatgt
     2341 tcttctttta ttctctcaag attttcaggc tgtatattaa aacttatatt aagaactatg
     2401 ctaaccacct catcaggaac cgttgtaggt ggcgtgggtt ttcttggcaa tcgactctca
     2461 tgaaaactac gagctaaata ttcaatatgt tcctcttgac caactttatt ctgcattttt
     2521 tttgaacgag gtttagagca agcttcagga aactgagaca ggaattttat taaaaattta
     2581 aattttgaag aaagttcagg gttaatagca tccatttttt gctttgcaag ttcctcagca
     2641 ttcttaacaa aagacgtctc ttttgacatg tttaaagttt aaacctcctg tgtgaaattg
     2701 ttatccgctc acaattccac acattatacg agccggaagc ataaagtgta aagcctgggg
     2761 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     2821 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     2881 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     2941 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3001 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3061 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3121 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3181 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3241 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3301 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3361 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3421 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3481 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3541 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3601 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     3661 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     3721 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     3781 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     3841 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     3901 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     3961 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     4021 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     4081 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     4141 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     4201 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     4261 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     4321 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     4381 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     4441 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     4501 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     4561 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     4621 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     4681 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     4741 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     4801 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     4861 ctataaaaat aggcgtatca cgaggcc
//
