LOCUS       V005352                13009 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005352
VERSION     V005352
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 13009)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13009)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        10..38
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             complement(869..1618)
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     CDS             1837..2415
                     /gene="luxI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Aliivibrio
                     fischeri. Accession#: P12747"
     CDS             2472..3911
                     /codon_start=1
                     /note="unnamed protein product; luxC"
                     /protein_id="SJL88052.1"
                     /translation="MNKCIPMIINGMIQDFDNYAYKEVKLNNDNRVKLSVITESSVSKT
                     LNIKDRINLNLNQIVNFLYTVGQRWKSEEYNRRRTYIRELKTYLGYSDEMARLEANWIA
                     MLLCSKSALYDIVNYDLGSIHVLDEWLPRGDCYVKAQPKGVSVHLLAGNVPLSGVTSIL
                     RAILTKNECIIKTSSSDPFTANALVSSFIDVNADHPITKSMSVMYWPHDEDMTLSQRIM
                     NHADVVIAWGGDEAIKWAVKYSPPHVDILKFGPKKSLSIIEAPKDIEAAAMGVAHDICF
                     YDQQACFSTQDVYYIGDNLPLFLNELEKQLDRYAKILPKGSNSFDEKAAFTLTEKESLF
                     AGYEVRKGDKQAWLIVVSPTNSFGNQPLSRSVYVHQVSDIKEIIPFVNKNRTQTVSIYP
                     WEASLKYRDKLARSGVERIVESGMNNIFRVGGAHDSLSPLQYLVRFVSHERPFNYTTKD
                     VAVEIEQTRYLEEDKFLVFVP"
     CDS             3926..4849
                     /codon_start=1
                     /note="unnamed protein product; luxD"
                     /protein_id="SJL88053.1"
                     /translation="MKDESAFFTIDHIIKLDNGQSIRVWETLPKKNVPEKKHTILIASG
                     FARRMDHFAGLAEYLSTNGFHVIRYDSLHHVGLSSGCINEFTMSIGKNSLLTVVDWLTD
                     HGVERIGLIAASLSARIAYEVVNKIKLSFLITAVGVVNLRDTLEKALEYDYLQLPISEL
                     PEDLDFEGHNLGSEVFVTDCFKHNWDTLDSTLNSVKGLAIPFIAFTANDDSWVKQSEVI
                     ELIDSIESSNCKLYSLIGSSHDLGENLVVLRNFYQSVTKAALALDDGLLDLEIDIIEPR
                     FEDVTSITVKERRLKNEIENELLELA"
     CDS             4880..5944
                     /codon_start=1
                     /note="unnamed protein product; luxA"
                     /protein_id="SJL88054.1"
                     /translation="MKFGNICFSYQPPGETHKQVMDRFVRLGIASEEVGFDTYWTLEHH
                     FTEFGLTGNLFVAAANLLGRTKTLNVGTMGVVIPTAHPVRQLEDVLLLDQMSKGRFNFG
                     TVRGLYHKDFRVFGVDMEESRAITQNFYQMIMESLQTGTISSDSDYIQFPKVDVYPKVY
                     SKNVPTCMTAESASTTEWLAIQGLPMVLSWIIGTNEKKAQMELYNEIATEYGHDISKID
                     HCMTYICSVDDDAQKAQDVCREFLKNWYDSYVNATNIFNDSNQTRGYDYHKGQWRDFVL
                     QGHTNTNRRVDYSNGINPVGTPEQCIEIIQRDIDATGITNITCGFEANGTEDEIIASMR
                     RFMTQVAPFLKEPK"
     CDS             5983..6963
                     /codon_start=1
                     /note="unnamed protein product; luxB"
                     /protein_id="SJL88055.1"
                     /translation="MKFGLFFLNFQKDGITSEETLDNMVKTVTLIDSTKYHFNTAFVNE
                     HHFSKNGIVGAPITAAGFLLGLTNKLHIGSLNQVITTHHPVRVAEEASLLDQMSEGRFI
                     LGFSDCESDFEMEFFRRHISSRQQQFEACYEIINDALTTGYCHPQNDFYDFPKVSINPH
                     CYSENGPKQYVSATSKEVVMWAAKKALPLTFKWEDNLETKERYAILYNKTAQQYGIDIS
                     DVDHQLTVIANLNADRSTAQEEVREYLKDYITETYPQMDRDEKINCIIEENAVGSHDDY
                     YESTKLAVEKTGSKNILLSFESMSDIKDVKDIIDMLNQKIEMNLP"
     CDS             7038..8174
                     /codon_start=1
                     /note="unnamed protein product; luxE"
                     /protein_id="SJL88056.1"
                     /translation="MTVHTEYKRNQIIASSEIDDLIFMTKPQEWSFEEQKEIRDKLVRE
                     AFYFHYNRNEEYRNYCINQHVSDNLHTIDEIPVFPTSVFKYKKLHTVTAEDIENWYTSS
                     GTRGVKSHIARDRLSIERLLGSVNFGMKYVGDWFEHQMELINLGPDRFNTNNIWFKYVM
                     SLVELLYPTEFTVDNDKIDFEKTVKHLFRIKNSKKDICLIGPPFFVYLLCQYMKENNIE
                     FKGGDRVHIITGGGWKSNQNDSLDRADFNQLLMDTFQLDKINQIRDTFNQVELNTCFFE
                     DEFQRKHVPPWVYARALDPETLKPVADGEIGLLSYMDASSTAYPAFIVTDDIGIVKEIR
                     EPDPYPGVTVEIVRRLNTRAQKGCALSMANVIQKNIKD"
     CDS             8178..8888
                     /codon_start=1
                     /note="unnamed protein product; luxG"
                     /protein_id="SJL88057.1"
                     /translation="MIVDGRVSKIVLASIRNNIYKVFITVNSPIKFIAGQFVMVTINGK
                     KCPFSIANCPTKNYEIELHIGSSNRDCSLDIIEYFVDALVEEVAIELDAPHGNAWLRSE
                     SNNPLLLIAGGTGLSYINSILTNCLNRNIPQDIYLYWGVKDSSLLYEDEELLNLSLNNK
                     NFHYIPVIEDKSEEWIGRKGTVLDAVMEDFTDLTYFDIYVCGPFMMAKTAKEKLIEEKK
                     AKSEQMFADAFAYV"
     regulatory      8889..8928
                     /label="luxICDABEG terminator"
                     /note="luxICDABEG terminator"
                     /regulatory_class="terminator"
     CDS             10563..10751
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    10856..10996
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(11182..11770)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(11944..12801)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(12802..12906)
                     /label="AmpR promoter"
ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
      421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
      481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
      541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
      601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccttc
      661 ctggttcaga gcctcatatc catattacga ccacttcaat gggcgatcaa aaagtacatt
      721 acatgatttt ttgcccaaca gaaaaagcct ctcatttaga gcagcttatt cgtcaagatt
      781 tcatggaaat gtatgagatc aattttccac aaaaaacaga gtaacaaaga gaaaaaccta
      841 taggtataaa gctttactta cgtacttaat ttttaaagta tgggcaatca attgctcctg
      901 ttaaaattgc tttagaaata ctttggcagc ggtttgttgt attgagtttc atttgcgcat
      961 tggttaaatg gaaagtgaca gtacgctcac tgcagcctaa tatttttgaa atatcccaag
     1021 agctttttcc ttcgcatgcc cacgctaaac attctttttc tcttttggtt aaatcgttgt
     1081 ttgatttatt atttgctata tttatttttc gataattatc aactagagaa ggaacaatta
     1141 atggtatgtt catacacgca tgtaaaaata aactatctat atagttgtct ttttctgaat
     1201 gtgcaaaact aagcattccg aagccattgt tagccgtatg aatagggaaa ctaaacccag
     1261 tgataagacc tgatgttttc gcttctttaa ttacatttgg agatttttta tttacagcat
     1321 tgttttcaaa tatattccaa ttaattggtg aatgattgga gttagaataa tctactatag
     1381 gatcatattt tattaaatta gcgtcatcat aatattgcct ccatttttta gggtaattat
     1441 ctagaattga aatatcagat ttaaccatag aatgaggata aatgatcgcg agtaaataat
     1501 attcacaatg taccatttta gtcatatcag ataagcattg attaatatca ttattgcttc
     1561 tacaagcttt aattttatta attattctgt atgtgtcgtc ggcatttatg tttttcatac
     1621 ccatctcttt atccttacct attgtttgtc gcaagttttg cgtgttatat atcattaaaa
     1681 cggtaatgga ttgacatttg attctaataa attggatttt tgtcacacta ttgtatcgct
     1741 gggaatacaa ttacttaaca taagcacctg taggatcgta caggtttacg caagaaaatg
     1801 gtttgttata gtcgaataaa cgcaagggag gttggtatga ctataatgat aaaaaaatcg
     1861 gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg ttatcaagtg
     1921 tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc agatgagtat
     1981 gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt aagtggatgc
     2041 tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc tgaattgctt
     2101 ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt tgctgtaggt
     2161 aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact atttgaagct
     2221 atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac atcaacagca
     2281 atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga caaagaaatt
     2341 catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga acagtttaaa
     2401 aaagcagtct taaattaatg ttgttaaatc attaatttat tttaaatact aagtatatta
     2461 tagggaaaat aatgaataaa tgtattccaa tgataattaa tggaatgatt caagattttg
     2521 ataattatgc atataaagaa gttaaactaa ataatgataa tagagtaaaa ttatctgtca
     2581 ttactgaaag ttcagtttca aaaacattaa atatcaaaga tagaattaat ctaaatttaa
     2641 atcagattgt gaatttttta tataccgttg gtcaacgatg gaaaagtgaa gaatataatc
     2701 ggcgacgaac ctatatccgt gagttaaaaa catatcttgg ttattctgat gaaatggcaa
     2761 gattagaagc gaattggatt gcaatgttat tgtgctctaa aagtgctttg tatgacattg
     2821 ttaattatga tttgggctct atacacgtat tagatgaatg gcttccacgt ggtgattgct
     2881 atgttaaagc acaaccgaaa ggtgtttctg ttcacttgtt agctggtaat gttccattat
     2941 caggagtgac atctattttg cgtgctattt taacaaaaaa tgagtgcatt attaaaactt
     3001 cgtcttcaga tccttttact gcaaacgctt tagtttccag ttttattgat gttaatgcag
     3061 accatccaat aaccaaatca atgtctgtta tgtattggcc gcatgatgaa gatatgactc
     3121 tatctcaaag aataatgaat catgccgacg tggttattgc ttggggtgga gacgaggcga
     3181 ttaaatgggc ggtaaaatat tcaccaccgc atgtcgatat tctgaaattt ggaccaaaga
     3241 aaagcttaag tattattgaa gctcctaaag atatagaagc cgcagcaatg ggggttgctc
     3301 atgatatttg tttctatgac cagcaagcct gcttctctac tcaagacgtt tattatatag
     3361 gagataattt acctttattt ttaaatgaac ttgaaaaaca gctagatcga tacgcgaaaa
     3421 ttttaccaaa aggttcaaat agttttgatg aaaaagcggc gtttactctt actgaaaaag
     3481 aaagtctatt tgctggatat gaagtgagaa agggagataa gcaagcttgg ttaatagtcg
     3541 tatcacctac aaatagcttt ggaaatcaac cgctatcacg aagtgtgtat gttcatcaag
     3601 tatctgatat taaagagata attccttttg ttaataaaaa tagaacacaa actgtttcta
     3661 tttatccttg ggaagcgtca ttaaaatatc gagataaatt agcaagaagt ggagttgaaa
     3721 gaattgttga atcaggcatg aataatattt tcagagttgg aggggctcat gattcattat
     3781 ctcctctcca gtacctagtt aggtttgtat cgcatgagag accatttaat tatacgacaa
     3841 aagatgttgc ggttgaaatc gaacaaacac gttacttaga ggaagataag tttttagttt
     3901 ttgtcccata gttaaaggaa attatatgaa agatgaaagt gcttttttta cgattgatca
     3961 catcatcaag cttgataatg gtcagtctat ccgagtttgg gaaacactcc ctaaaaagaa
     4021 cgtaccagag aaaaaacata caatacttat tgcttcgggt tttgctagaa gaatggatca
     4081 ttttgcaggt cttgctgagt atttatctac taacggtttt catgtcattc gctacgattc
     4141 tttgcatcat gttggattaa gcagtggatg tataaatgaa tttacgatgt cgattggaaa
     4201 aaatagcctg cttacagtcg tagattggct tacagatcat ggtgtcgaac gaatagggct
     4261 gattgctgct agtttgtcag cgagaatcgc ctatgaggta gtaaataaaa ttaaattatc
     4321 atttttaatt acggccgtag gtgtcgttaa tcttagagat acattagaaa aagcattgga
     4381 gtatgactat ttgcaattac ctatttcaga gttaccagaa gatcttgact ttgaaggtca
     4441 taatttagga tcggaggtct ttgttacaga ttgctttaaa cataattggg acacgttaga
     4501 ctcgacactt aatagtgtta aaggattagc gattccattt attgctttta ctgcaaacga
     4561 tgatagttgg gtaaagcaaa gtgaagttat agagctcatt gatagcattg aatctagtaa
     4621 ttgtaagctc tattcgctaa ttggaagttc acatgatctt ggggaaaatt tggttgtatt
     4681 aagaaatttt tatcaatcag tgacgaaggc agccttagca ttagatgatg gtttattgga
     4741 tttagagata gacattattg aacctcgatt tgaggacgtt acaagtatta ctgttaagga
     4801 gcgtagatta aaaaatgaaa ttgaaaatga attattagaa ttggcttaaa taaacagaat
     4861 caccaaaaag gaatagagta tgaagtttgg aaatatttgt ttttcgtatc aaccaccagg
     4921 tgaaactcat aagcaagtaa tggatcgctt tgttcggctt ggtatcgcct cagaagaggt
     4981 agggtttgat acatattgga ccttagaaca tcattttaca gagtttggtc ttacgggaaa
     5041 tttatttgtt gctgcggcta acctgttagg aagaactaaa acattaaatg ttggcactat
     5101 gggggttgtt attccgacag cacacccagt tcgacagtta gaagacgttt tattattaga
     5161 tcaaatgtcg aaaggtcgtt ttaattttgg aaccgttcga gggctatacc ataaagattt
     5221 tcgagtattt ggtgttgata tggaagagtc tcgagcaatt actcaaaatt tctaccagat
     5281 gataatggaa agcttacaga caggaaccat tagctctgat agtgattaca ttcaatttcc
     5341 taaggttgat gtatatccca aagtgtactc aaaaaatgta ccaacctgta tgactgctga
     5401 gtccgcaagt acgacagaat ggctagcaat acaagggcta ccaatggttc ttagttggat
     5461 tattggtact aatgaaaaaa aagcacagat ggaactctat aatgaaattg cgacagaata
     5521 tggtcatgat atatctaaaa tagatcattg tatgacttat atttgttctg ttgatgatga
     5581 tgcacaaaag gcgcaagatg tttgtcggga gtttctgaaa aattggtatg actcatatgt
     5641 aaatgcgacc aatatcttta atgatagcaa tcaaactcgt ggttatgatt atcataaagg
     5701 tcaatggcgt gattttgttt tacaaggaca tacaaacacc aatcgacgtg ttgattatag
     5761 caatggtatt aaccctgtag gcactcctga gcagtgtatt gaaatcattc aacgtgatat
     5821 tgatgcaacg ggtattacaa acattacatg cggatttgaa gctaatggaa ctgaagatga
     5881 aataattgct tccatgcgac gctttatgac acaagtcgct cctttcttaa aagaacctaa
     5941 ataaattact tatttgatac tagagataat aaggaacaag ttatgaaatt tggattattt
     6001 tttctaaact ttcagaaaga tggaataaca tctgaagaaa cgttggataa tatggtaaag
     6061 actgtcacgt taattgattc aactaaatat cattttaata ctgcctttgt taatgaacat
     6121 cacttttcaa aaaatggtat tgttggagca cctattaccg cagctggttt tttattaggg
     6181 ttaacaaata aattacatat tggttcatta aatcaagtaa ttaccaccca tcaccctgta
     6241 cgtgtagcag aagaagccag tttattagat caaatgtcag agggacgctt cattcttggt
     6301 tttagtgact gcgaaagtga tttcgaaatg gaatttttta gacgtcatat ctcatcaagg
     6361 caacaacaat ttgaagcatg ctatgaaata attaatgacg cattaactac aggttattgt
     6421 catccccaaa acgactttta tgattttcca aaggtttcaa ttaatccaca ctgttacagt
     6481 gagaatggac ctaagcaata tgtatccgct acatcaaaag aagtcgtcat gtgggcagcg
     6541 aaaaaggcac tgcctttaac atttaagtgg gaggataatt tagaaaccaa agaacgctat
     6601 gcaattctat ataataaaac agcacaacaa tatggtattg atatttcgga tgttgatcat
     6661 caattaactg taattgcgaa cttaaatgct gatagaagta cggctcaaga agaagtgaga
     6721 gaatacttaa aagactatat cactgaaact taccctcaaa tggacagaga tgaaaaaatt
     6781 aactgcatta ttgaagagaa tgcagttggg tctcatgatg actattatga atcgacaaaa
     6841 ttagcagtgg aaaaaacagg gtctaaaaat attttattat cctttgaatc aatgtccgat
     6901 attaaagatg taaaagatat tattgatatg ttgaaccaaa aaatcgaaat gaatttacca
     6961 taataaaatt aaaggcaatt tctatattag attgcctttt taaatttctg ttgatattag
     7021 gtattactgg agagggtatg actgtccata ctgaatataa aagaaatcaa atcattgcta
     7081 gttcagaaat tgatgatctt atctttatga cgaaaccaca agagtggtca tttgaagagc
     7141 aaaaagaaat acgggataaa ttagttcgtg aggcttttta ttttcactac aatagaaatg
     7201 aagaatatag aaattattgt atcaatcagc atgtgagtga taatttacac actattgatg
     7261 aaatacccgt gtttccaaca tctgttttta aatataagaa attacatact gtcacagccg
     7321 aggacattga aaattggtat acaagtagtg gaactcgtgg agtaaaaagt catattgcac
     7381 gtgatcgtct tagcattgaa cgcttgcttg gttctgtcaa cttcggaatg aaatacgttg
     7441 gagattggtt tgagcatcaa atggaattga taaatttagg accagataga ttcaatacaa
     7501 ataatatttg gtttaaatat gtcatgagtt tggtcgagtt actttatccg actgaattta
     7561 cagttgataa tgacaaaata gattttgaaa aaacagtaaa acatctattt agaattaaga
     7621 atagtaaaaa agacatttgc ttaattgggc caccattttt tgtgtatctt ttgtgccaat
     7681 atatgaaaga aaacaatatt gaatttaaag gaggagatag agtacatatt attactggtg
     7741 gaggatggaa atctaatcag aatgactctt tagatcgtgc tgattttaat caattattaa
     7801 tggatacttt ccaactcgac aagattaatc aaattagaga tacctttaat caagttgagc
     7861 ttaatacttg tttttttgaa gatgaatttc aaagaaaaca tgttccaccg tgggtatatg
     7921 ctcgggctct tgatcctgaa accttgaaac ccgtagcaga tggtgagatc gggttgttaa
     7981 gttatatgga cgcctcatca actgcttacc ctgcttttat tgttactgat gatatcggta
     8041 ttgtaaaaga aattagagaa ccagatcctt acccaggggt aactgttgag attgttcggc
     8101 gcttaaatac acgtgcgcaa aaaggatgcg cgctctctat ggctaatgtc atacaaaaga
     8161 atatcaagga ttaagttatg attgttgatg gtagagtttc aaaaatagta ttagcgtcaa
     8221 taagaaataa tatatataag gtatttatta ctgtaaattc accaataaag ttcatcgctg
     8281 gacaatttgt aatggtcacg attaatggga aaaaatgccc tttttcaatt gcgaattgcc
     8341 cgacaaaaaa ttacgaaata gaattgcata ttggtagttc gaatagagac tgctcattgg
     8401 atattatcga atattttgtc gatgctcttg ttgaggaagt cgcaattgag ttagatgctc
     8461 cccatggaaa cgcttggtta cggtctgaaa gtaataaccc attgctatta attgcgggag
     8521 gtacaggttt atcatatata aatagcattc taacaaattg cttaaatagg aatatacctc
     8581 aagatattta tctttactgg ggagtaaaag acagttctct tttgtatgaa gatgaagagt
     8641 tactaaactt atcactaaac aacaaaaact ttcattatat tcctgttatt gaagataaaa
     8701 gtgaagaatg gatagggaga aaaggcactg ttcttgatgc tgtcatggaa gattttactg
     8761 atcttactta ttttgatatt tatgtttgtg gacccttcat gatggctaaa acagcaaaag
     8821 aaaaattaat tgaagagaaa aaagcaaagt cagaacagat gtttgccgat gcttttgcat
     8881 acgtataaag agaatataaa aagccagatt attaatccgg cttttttatt atttcaatgt
     8941 gtccaagggt aaccaagtta cttcaactcc cgcctgagag aacatatctt ggctcacttg
     9001 aattttttca ccccaacgag ataagaagtc ttcagtttgt tcagggcaat atacttttga
     9061 tattcctgtt tggatgattt ttgctgcgca gtttggacaa gggaagtgag taacccaaat
     9121 atcgcaacct tctaaatcac gcttggcaaa taaaatggca ttttcttcag catgaagtgt
     9181 ttttaaatat ttaatttcac gttcatctgt atctgcactg tctgatacac catgtgggta
     9241 gccattaaat ccaacagaaa caatacggtt atgcttggtg attactgcac caacttgagt
     9301 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     9361 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     9421 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     9481 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     9541 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     9601 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     9661 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     9721 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     9781 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     9841 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     9901 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     9961 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
    10021 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
    10081 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
    10141 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
    10201 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
    10261 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
    10321 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
    10381 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
    10441 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
    10501 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
    10561 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
    10621 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
    10681 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
    10741 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
    10801 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
    10861 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
    10921 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
    10981 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
    11041 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
    11101 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
    11161 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
    11221 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
    11281 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
    11341 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
    11401 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
    11461 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    11521 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    11581 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    11641 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    11701 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    11761 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    11821 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    11881 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    11941 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
    12001 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
    12061 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
    12121 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
    12181 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
    12241 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
    12301 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
    12361 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
    12421 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
    12481 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
    12541 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
    12601 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
    12661 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
    12721 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
    12781 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
    12841 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
    12901 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
    12961 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa
//
