LOCUS       40924_25996        5847 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pJDL, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Park JB.
  TITLE     Concurrent measurement of promoter activity and transfection 
            efficiency using a new reporter vector containing both Photinus 
            pyralis and Renilla reniformis luciferase genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Park JB.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-OCT-2000) PL, BHNRC, ARS, USDA, Barc-East, Bldg. 307, 
            Rm. 313, Beltsville, MD 20705, USA
REFERENCE   3  (bases 1 to 5847)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5847)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-OCT-2000) PL, BHNRC, ARS, USDA, Barc-East, Bldg. 307, Rm. 313, 
            Beltsville, MD 20705, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             74..1723
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     polyA_signal    1862..1983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        2058..2361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2362..2565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2631..2763
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        2808..2826
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2836..3768
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(3802..3923)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4056..4160
                     /label=AmpR promoter
     CDS             4161..5018
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5192..5780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctcgag cggccgccag tgtgatggat ccgagctcgg taccaagctt ggcattccgg
       61 tactgttggt aaaatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc
      121 tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt
      181 tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt acgcggaata
      241 cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca
      301 cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt
      361 atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa
      421 cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat
      481 tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg attctaaaac
      541 ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt
      601 taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg cactgataat
      661 gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata gaactgcctg
      721 cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc
      781 gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt
      841 gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttttacgatc
      901 ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt cattcttcgc
      961 caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg cttctggggg
     1021 cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc ttccagggat
     1081 acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga
     1141 tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct
     1201 ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca gaggacctat
     1261 gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg
     1321 atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct tcatagttga
     1381 ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg aattggaatc
     1441 gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga
     1501 cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa
     1561 agagatcgtg gattacgtgg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt
     1621 tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag
     1681 agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaaagat cgggatccac
     1741 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
     1801 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     1861 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     1921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     1981 ttaacgcgta gaattcaccc ttattaatag taatcaatta cggggtcatt agttcatagc
     2041 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     2101 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2161 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2221 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2281 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2341 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2401 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2461 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2521 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2581 cagatccgct agcggggtac tgcagaagtt ggtcgtgagg cactgggcag gtaagtatca
     2641 aggttacaag acaggtttaa ggagaccaat agaaactggg cttgtcgaga cagagaagac
     2701 tcttgcgttt ctgataggca cctattggtc ttactgacat ccactttgcc tttctctcca
     2761 caggtgtcca ctcccagttc aattacagct cttaaggcta gagtacttaa tacgactcac
     2821 tataggctag ccaccatgac ttcgaaagtt tatgatccag aacaaaggaa acggatgata
     2881 actggtccgc agtggtgggc cagatgtaaa caaatgaatg ttcttgattc atttattaat
     2941 tattatgatt cagaaaaaca tgcagaaaat gctgttattt ttttacatgg taacgcggcc
     3001 tcttcttatt tatggcgaca tgttgtgcca catattgagc cagtagcgcg gtgtattata
     3061 ccagacctta ttggtatggg caaatcaggc aaatctggta atggttctta taggttactt
     3121 gatcattaca aatatcttac tgcatggttt gaacttctta atttaccaaa gaagatcatt
     3181 tttgtcggcc atgattgggg tgcttgtttg gcatttcatt atagctatga gcatcaagat
     3241 aagatcaaag caatagttca cgctgaaagt gtagtagatg tgattgaatc atgggatgaa
     3301 tggcctgata ttgaagaaga tattgcgttg atcaaatctg aagaaggaga aaaaatggtt
     3361 ttggagaata acttcttcgt ggaaaccatg ttgccatcaa aaatcatgag aaagttagaa
     3421 ccagaagaat ttgcagcata tcttgaacca ttcaaagaga aaggtgaagt tcgtcgtcca
     3481 acattatcat ggcctcgtga aatcccgtta gtaaaaggtg gtaaacctga cgttgtacaa
     3541 attgttagga attataatgc ttatctacgt gcaagtgatg atttaccaaa aatgtttatt
     3601 gaatcggacc caggattctt ttccaatgct attgttgaag gtgccaagaa gtttcctaat
     3661 actgaatttg tcaaagtaaa aggtcttcat ttttcgcaag aagatgcacc tgatgaaatg
     3721 ggaaaatata tcaaatcgtt cgttgagcga gttctcaaaa atgaacaata attctagagc
     3781 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     3841 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3901 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3961 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
     4021 ataaggatcc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4081 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4141 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4201 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4261 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4321 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4381 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4441 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4501 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4561 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4621 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4681 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4741 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4801 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4861 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4921 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4981 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5041 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5101 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5161 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5221 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5281 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5341 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5401 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5461 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5521 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5581 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5641 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5701 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5761 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5821 gctggccttt tgctcacatg gctcgac
//
