LOCUS       62056_13110        4950 bp DNA     circular SYN 31-MAY-2020
DEFINITION  Expression vector pJDHSP, complete sequence.
ACCESSION   KX267661
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Jia B, Hu Z, Huang Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2016) College of Life Sciences, Shenzhen 
            University, Shenzhen Key Laboratory of Marine Bioresource and 
            Eco-Environmental Science, 3688, Nanhai Road, Shenzhen, Guangdong 
            518060, China
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (19-MAY-2016) College of Life Sciences, Shenzhen University, 
            Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental 
            Science, 3688, Nanhai Road, Shenzhen, Guangdong 518060, China"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     381..397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      476..742
                     /note="Chlamydomonas reinhardtii heat shock protein 70A 
                     (HSP70Ap)"
                     /regulatory_class="promoter"
     regulatory      748..972
                     /note="Chlamydomonas reinhardtii ribulose-1,5-bisphosphate 
                     carboxylase/oxygenase small subunit 2 (RBCS2p)"
                     /regulatory_class="promoter"
     misc_feature    970..1126
                     /label=5' sequence of RBCS2 gene containing intron 1
                     /note="5' sequence of RBCS2 gene containing intron 1"
     misc_feature    1127..1168
                     /note="multiple cloning sites in frame with the 5'
                     sequences of RBCS2 gene"
     misc_feature    1200..1452
                     /note="Chlamydomonas reinhardtii ribulose-1,5-bisphosphate 
                     carboxylase/oxygenase small subunit 2 3' UTR"
     misc_feature    1475..2611
                     /label=zeocin resistance gene expression cassette
                     /note="zeocin resistance gene expression cassette"
     primer_bind     complement(2729..2745)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2753..2769)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2777..2807)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2822..2843)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3131..3719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3893..4750)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4751..4855)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
      241 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      301 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      361 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aaagaagcat gacggcaagt
      421 ggacgattgg catcgtagtg caagagcatg acggcagtgg acgattacta gatctgctga
      481 ggcttgacat gattggtgcg tatgtttgta tgaagctaca ggactgattt ggcgggctat
      541 gagggcgggg gaagctctgg aagggccgcg atggggcgcg cggcgtccag aaggcgccat
      601 acggcccgct ggcggcaccc atccggtata aaagcccgcg accccgaacg gtgacctcca
      661 ctttcagcga caaacgagca cttatacata cgcgactatt ctgccgctat acataaccac
      721 tcagctagct taagatccca tccctagggc atgccgggcg cgccagaagg agcgcagcca
      781 aaccaggatg atgtttgatg gggtatttga gcacttgcaa cccttatccg gaagccccct
      841 ggcccacaaa ggctaggcgc caatgcaagc agttcgcatg cagcccctgg agcggtgccc
      901 tcctgataaa ccggccaggg ggcctatgtt ctttactttt ttacaagaga agtcactcaa
      961 catcttaaaa tggccaggtg agtcgacgag caagcccggc ggatcaggca gcgtgcttgc
     1021 agatttgact tgcaacgccc gcattgtgtc gacgaaggct tttggctcct ctgtcgctgt
     1081 ctcaagcagc atctaaccct gcgtcgccgt ttccatttgc aggatgaagc ttgctagcca
     1141 tatgcacgtg gaattcctgc agggtacccc gacgtcgacc cactctagag gatcgatccc
     1201 cgctccgtgt aaatggaggc gctcgttgat ctgagccttg ccccctgacg aacggcggtg
     1261 gatggaagat actgctctca agtgctgaag cggtagctta gctccccgtt tcgtgctgat
     1321 cagtcttttt caacacgtaa aaagcggagg agttttgcaa ttttgttggt tgtaacgatc
     1381 ctccgttgat tttggcctct ttctccatgg gcgggctggg cgtatttggt aggacatcag
     1441 cgactaagca ctggatccat aggcctcttt aaatgccaga aggagcgcag ccaaaccagg
     1501 atgatgtttg atggggtatt tgagcacttg caacccttat ccggaagccc cctggcccac
     1561 aaaggctagg cgccaatgca agcagttcgc atgcagcccc tggagcggtg ccctcctgat
     1621 aaaccggcca gggggcctat gttctttact tttttacaag agaagtcact caacatctta
     1681 aaatggccag gtgagtcgac gagcaagccc ggcggatcag gcagcgtgct tgcagatttg
     1741 acttgcaacg cccgcattgt gtcgacgaag gcttttggct cctctgtcgc tgtctcaagc
     1801 agcatctaac cctgcgtcgc cgtttccatt tgcaggatgg ccaagctgac cagcgccgtt
     1861 ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg
     1921 ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg
     1981 ttcatcagcg cggtccagga ccaggtgagt cgacgagcaa gcccggcgga tcaggcagcg
     2041 tgcttgcaga tttgacttgc aacgcccgca ttgtgtcgac gaaggctttt ggctcctctg
     2101 tcgctgtctc aagcagcatc taaccctgcg tcgccgtttc catttgcagg accaggtggt
     2161 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     2221 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     2281 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     2341 cgtggccgag gagcaggact aaccgacgtc gacccactct agaggatcga tccccgctcc
     2401 gtgtaaatgg aggcgctcgt tgatctgagc cttgccccct gacgaacggc ggtggatgga
     2461 agatactgct ctcaagtgct gaagcggtag cttagctccc cgtttcgtgc tgatcagtct
     2521 ttttcaacac gtaaaaagcg gaggagtttt gcaattttgt tggttgtaac gatcctccgt
     2581 tgattttggc ctctttctcc atgggcgggc tgggcgtatt tggcggccgc aatctccaga
     2641 ggatcgccgg gaaccgagga cgagtcgtaa tcagtcaggt cccatctcca gaggatcgcc
     2701 gggaaccgag gacgagttcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     2761 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     2821 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     2881 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2941 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3001 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3061 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3541 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     3601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3661 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4021 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4081 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4141 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4201 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4261 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4321 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4381 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4441 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4501 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4561 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4621 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4681 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4741 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4801 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4861 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4921 aaaataggcg tatcacgagg ccctttcgtc
//
