LOCUS       40924_25991        6551 bp DNA     circular SYN 18-DEC-2018
DEFINITION  MTL KO vector pJD1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6551)
  AUTHORS   Lin CH, Kabrawala S, Fox EP, Nobile CJ, Johnson AD, Bennett RJ.
  TITLE     Genetic Control of Conventional and Pheromone-Stimulated Biofilm 
            Formation in Candida albicans
  JOURNAL   PLoS Pathog. 9 (4), E1003305 (2013)
  PUBMED    23637598
REFERENCE   2  (bases 1 to 6551)
  AUTHORS   Lohse MB.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-AUG-2012) Biochemistry and Biophysics, UCSF, Genentech
            Hall N374, 600 16th Street, San Francisco, CA 94143, USA
REFERENCE   3  (bases 1 to 6551)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6551)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013"; volume: "9"; issue: "4"; pages: "E1003305"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-AUG-2012) Biochemistry and Biophysics, UCSF, Genentech Hall 
            N374, 600 16th Street, San Francisco, CA 94143, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    7..530
                     /label=3'flanking region of MTL
                     /note="3'flanking region of MTL"
     primer_bind     531..550
                     /label=UP5 linker region
                     /note="UP5 linker region"
     CDS             1627..3021
                     /codon_start=1
                     /product="Arg4"
                     /EC_number="1.2.3.4"
                     /label=Arg4
                     /note="argininosuccinate lyase"
                     /protein_id="AGG09091.1"
                     /translation="MSQQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDADLTGTK
                     VYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRLGEIIGK
                     NISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDVLMPGYTH
                     LQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGIDREFLAKG
                     LGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFGFIKLADAYS
                     TGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQEDKEPLFDALT
                     TVEHSILIATGVISTLLIDKQNMEKALTMDMLATDLADYLVRKGVPFRETHHISGECVR
                     KAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDAIGGTAKSAVLKQLEN
                     LKSILN"
     primer_bind     3838..3857
                     /label=UP2 linker region
                     /note="UP2 linker region"
     misc_feature    3858..4340
                     /label=5'flanking region of MTL
                     /note="5'flanking region of MTL"
     primer_bind     complement(4394..4410)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4418..4434)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4442..4472)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4487..4508)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4796..5384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5558..6415)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6416..6520)
                     /label=AmpR promoter
ORIGIN
        1 cccgggcatt ggaattattg tctaacctgc ccgtggtaat acaaaagaga aataaaccaa
       61 ttaatttgac aaaattcatt ccggcaatta tatcaggaat aattgataat aaacaattca
      121 attcaataga agaaaaattt gaatttttaa ttaaattctt actggatttg aaattaaaca
      181 atttcaataa taagcttaca atgaatgcaa gtgttaaatt aataaatgaa attattaaat
      241 ctagcaaagg taataaaatc aagaatacac atgacaatac tactaataat ataaattcac
      301 taaataccgc aactaagatc aacaataaaa gtcaattatt aaatttgatt caatcaaatc
      361 ccaaaatgaa acaagcaatt aaattaattg ttcaacatca taaaaataaa tcccattcat
      421 agtttataat gtatatacat ctatatacca ttcttctttc tttcacattt tcaataaata
      481 aataaagata ccactactat tactactact accaccactt atttccagtg gcagggatgc
      541 ggccgctgac agctcggatc cactagtaac ggccgccagt gtgctggaat tcgcccttaa
      601 gaacatttct gtaccgcaaa tgtatcgact ttttcgatgg tgtgttattg ttgttttgtt
      661 gcgaaatata atagcttctg ctttatatcc tttgtgttga tccttcccct cctttcatac
      721 ttgtaaaatt acaattggta ttttcaacca atattaccac acgtcatgca ctaactcaaa
      781 tgggcaaaaa aaagagagat aggaattacg tcattttatt atgtttttac gtggactaga
      841 aggttaagat aatttctggt tattttgttg ataattgagt tttgttcgac ctccaccaat
      901 ctttaaactc tcgttgagta ttcattttaa tgattttttg aaatcagaag gggtgtttct
      961 aacccaggat tgttttattg agaggttgaa acatcaatgt gttagtttat tgatttgggt
     1021 tggtttaata gaaaaatatc agtaggtgtt caataattgt agtttatttt cttggctaaa
     1081 attgttgcga aaaattgaca aaataataac aaaaagtatt ttagatgcta aaacataata
     1141 gtaggaatat ctacctgaac catttacatt caacaactgg caagaacctt cctaataatt
     1201 tgcaagagta atcacaatta aaccaaaatc aaagtttacc gtgtatatag ttggacagaa
     1261 agctgtcaca ttggtttaat acatagttga atatgtatgc atgaaacaac aacaatacaa
     1321 ttacagctca tgatccttca attaatttca acattcaaac aacataacac cttgtgtaaa
     1381 tgaggttatg agtacatgtg aacaacaaat cttatgacta aactcgggtc gtgtactttt
     1441 gacgattgag tcatttttac caattaggca attaattttt gctaaatttt cattaaccgc
     1501 gctggccatc ccaacaaacg atataaaagc tgacaacgaa aaaaaaaaaa aaaaaaactc
     1561 caattctgaa aaatttttct tcttcttcat actaaacaaa acaactataa agataatcaa
     1621 ttaataatgt cacaacaacc tagtgaaaat aaattatggg gtggtcgatt cactggcgcc
     1681 actgatccat taatggattt atacaatgct tcattacctt atgataaagt tatgtatgat
     1741 gcagatttaa ctggtactaa agtatatact caaggattga acaaattagg attaattacc
     1801 actgaagaat tgaatctgat tcatcaaggt ttagaacaaa ttagacaaga atggcatgat
     1861 aataaattta ttattaaagc tggtgatgaa gatattcata ctgctaatga acgtagatta
     1921 ggagaaatca ttgggaaaaa catttctggt aaagttcata ctggtagatc cagaaatgat
     1981 caagttgcta ctgatatgag aatttttgtt cgtgaatcat tacttaattt aagtaagatt
     2041 ttacatcaat tcattacagc aattcttgaa cgagctcata aagaaattga tgtattaatg
     2101 cctggttata ctcatttaca aaaagctcaa cctattagat gggctcattg gttaagttca
     2161 tatgcaactt atttcactga agattataaa cgtttacaag aaatcattac tcgtgttaat
     2221 caatctccat taggatctgg tgctttagct ggtcatccct acggtattga tcgtgaattt
     2281 ttagctaaag gattaggatt tgatggtgtt attgggaatt ctttaactgc agtttctgat
     2341 cgtgattttg ttgttgaaag tttattttgg agtacattat ttatgaatca tatttctcga
     2401 ttttcagaag atttaattat ttattctagt ggtgaatttg gatttataaa gttagctgat
     2461 gcttactcta ctggttcatc attaatgcca caaaagaaaa atcctgattc attagaatta
     2521 ttacgtggga aaagtggcag agtgtttggt cagttatctg ggtttttaat gtcaattaaa
     2581 tccattccat caacctataa taaagatatg caagaagata aagaaccatt atttgatgca
     2641 ttgactaccg tagaacattc aattttaatt gctactggtg tgatttctac tttactgatt
     2701 gataaacaaa atatggaaaa agctttaact atggatatgt tagctactga tttagcagat
     2761 tatttagtta gaaaaggtgt tccatttaga gaaactcatc atatttctgg tgaatgtgtt
     2821 agaaaagctg aagaagaaaa attgtcggga attgaccaat tatcatttga acaatttcaa
     2881 caaattgatt cacgttttga aaaagatgtt atggaaactt ttgattttga agccagtgtg
     2941 gaaagaagag atgctattgg tggtactgct aagagcgctg ttttaaaaca gttagaaaat
     3001 ttgaaatcaa ttttaaatta atttataaat aatcatatag tattcaatgt tttgttttgt
     3061 tttttttttt ttttttatgg aaatgtacaa acagtatata atcaattaat agaaagatac
     3121 cctgtattcc attgtatcgc ctttgggatg tctataatac caactttcaa gaatgagaaa
     3181 ttaaacaaca atttctttca ccaatcaact cgtctatatt caataaactc attctggtct
     3241 tttcaaatat ttaatgttat cctgatcaaa cttacaaatc aaaaatgtat caaaatcatt
     3301 cattagttct tcttcttttg ttatctttgt cactatattt ctctccatcc agaccaaaaa
     3361 tacttccatt gtgaagatcg tctgaatcat aatcagcaac tacctcttcc atttcagaga
     3421 attcagattt ttcaaaatag gtatcacggg ccaagtacac acaagacaaa cgatacgagt
     3481 gctgggatat ttctttcaat gtatcactaa attcgttgag atttaaagag agccctttga
     3541 ctgctttcat tactttcctc acatcatgag catccacatt caaatttcct gtagcccttt
     3601 attcttagtt ttcttttctt attcttttct tcgtacattg aaatcacaaa tggtgatatc
     3661 aaacgaaggt cacactgact tatgtctaat acctccaatg atgatactgg taacttaacc
     3721 aggtctactc ttttcaaacg atttctcatt agtgtcaatt tacgtaggtt cttcaacctt
     3781 tcaaacgatg caaatagctt ttgtaagggc gaattctgca gatatccatc acactggcac
     3841 ggcgcgccta gcagcgggta catacaaaac tggttattat aacaggacaa tgaatgagaa
     3901 acaaaacttc atatttaaat ttgactgtaa gacattgtaa ttctgtcatt atagtatttc
     3961 tattatataa tatgaaataa cacttcataa gaaaaagaaa aaaaaaagct agtataagat
     4021 acgaataaat aagcatataa tagtagtagt agaactttca tcaattttca taatatggtt
     4081 tgtttaacac caataattcc ataaccgttt gtacaatggt ttagctttat tagcggcttt
     4141 ttcttcttca atttcagctc ttataagatc caaatcaaag gatcttctgc cactatctaa
     4201 atcgatatct tccaatttga cacataattt ccaatttcga gtgtataatt tatggccaac
     4261 ataaaagatt aaaatgacaa ctgctcccaa ataattttgg aaaaagtttt cggcattggc
     4321 tttaccttta ctacccaagg cccggggatc ctctagagtc gacctgcagg catgcaagct
     4381 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     4441 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     4501 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     4561 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     4621 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     4681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     4741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     4801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     4861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     4921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     4981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     5041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     5101 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     5161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     5221 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     5281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     5341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     5401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     5461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     5521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     5581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     5641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     5701 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     5761 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     5821 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     5881 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     5941 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     6001 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     6061 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     6121 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     6181 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     6241 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     6301 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     6361 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     6421 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     6481 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     6541 cacctgacgt c
//
