LOCUS       40924_25986        3775 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Allelic replacement vector pJC84, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Child R, Edwards JA,
            Brouwer D, Nair V, Fischer ER, Wicke L, Curda AJ, Kupko JJ III, 
            Martens C, Crane DD, Bosio CM, Porcella SF, Celli J.
  TITLE     Intracellular biology and virulence determinants of Francisella 
            tularensis revealed by transcriptional profiling inside macrophages
  JOURNAL   Cell. Microbiol. 11 (7), 1128-1150 (2009)
  PUBMED    19388904
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Wehrly TD, Chong A, Virtaneva K, Sturdevant DE, Edwards JA, Nair V, 
            Fischer ER, Wicke L, Curda AJ, Kupko JJ III, Martens C, Crane DD, 
            Bosio CM, Porcella SF, Celli J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2008) Laboratory of Intracellular Parasites, 
            RML/NIAID/DIR/NIH, 903 South 4th street, Hamilton, MT 59840, USA
REFERENCE   3  (bases 1 to 3775)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3775)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 
            Microbiol."; date: "2009"; volume: "11"; issue: "7"; pages: 
            "1128-1150"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2008) Laboratory of Intracellular Parasites, 
            RML/NIAID/DIR/NIH, 903 South 4th street, Hamilton, MT 59840, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3775
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(80..1498)
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
     CDS             complement(1522..2313)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     regulatory      complement(2389..2658)
                     /label=Francisella tularensis groEL promoter
                     /note="Francisella tularensis groEL promoter"
                     /regulatory_class="promoter"
     misc_feature    2659..2715
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        complement(2725..2743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(3001..3589)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacgtcggat tcacctttat gttgataaga aataaaagaa aatgccaata ggatatcggc
       61 attttctttt gcgtttttat ttgttaactg ttaattgtcc ttgttcaagg atgctgtctt
      121 tgacaacaga tgttttcttg cctttgatgt tcagcaggaa gcttggcgca aacgttgatt
      181 gtttgtctgc gtagaatcct ctgtttgtca tatagcttgt aatcacgaca ttgtttcctt
      241 tcgcttgagg tacagcgaag tgtgagtaag taaaggttac atcgttagga tcaagatcca
      301 tttttaacac aaggccagtt ttgttcagcg gcttgtatgg gccagttaaa gaattagaaa
      361 cataaccaag catgtaaata tcgttagacg taatgccgtc aatcgtcatt tttgatccgc
      421 gggagtcagt gaacaggtac catttgccgt tcattttaaa gacgttcgcg cgttcaattt
      481 catctgttac tgtgttagat gcgatcagcg gtttcatcac ttttttcagt gtgtaatcat
      541 cgtttagctc aatcataccg agagcgccgt ttgctaactc agccgtgcgt tttttatcgc
      601 tttgcagaag tttttgactt tcttgacgga agaatgatgt gcttttgcca tagtatgctt
      661 tgttaaataa agattcttcg ccttggtagc catcttcagt tccagtgttt gcttcaaata
      721 ctaagtattt gtggccttta tcttctacgt agtgaggatc tctcagcgta tggttgtcgc
      781 ctgagctgta gttgccttca tcgatgaact gctgtacatt ttgatacgtt tttccgtcac
      841 cgtcaaagat tgatttataa tcctctacac cgttgatgtt caaagagctg tctgatgctg
      901 atacgttaac ttgtgcagtt gtcagtgttt gtttgccgta atgtttaccg gagaaatcag
      961 tgtagaataa acggattttt ccgtcagatg taaatgtggc tgaacctgac cattcttgtg
     1021 tttggtcttt taggatagaa tcatttgcat cgaatttgtc gctgtcttta aagacgcggc
     1081 cagcgttttt ccagctgtca atagaagttt cgccgacttt ttgatagaac atgtaaatcg
     1141 atgtgtcatc cgcattttta ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat
     1201 agtttgcgac agtgccgtca gcgttttgta atggccagct gtcccaaacg tccaggcctt
     1261 ttgcagaaga gatattttta attgtggacg aatcgaattc aggaacttga tatttttcat
     1321 ttttttgctg ttcagggatt tgcagcatat catggcgtgt aatatgggaa atgccgtatg
     1381 tttccttata tggcttttgg ttcgtttctt tcgcaaacgc ttgagttgcg cctcctgcca
     1441 gcagtgcggt agtaaaggtt agtactgttg cttgttttgc aaactttttg atgttcatcg
     1501 ttcatgtctc ctgctagctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     1561 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     1621 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     1681 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     1741 ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc
     1801 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     1861 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     1921 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     1981 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     2041 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     2101 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     2161 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     2221 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     2281 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gacctccttg cacgacgaac
     2341 taatactcta tcttgtaatg gacgaatgtt cataacaatc ttactccttt gttaaattat
     2401 ttttgtttaa gcattttgaa tttgtatgca tcttagaaca atctcaaagt tccaagctct
     2461 tgcaagtcta gatattgagt caaaaaaaaa aatttcaaga aatttttttg tttttttgtc
     2521 aaacaagctc tcgattttaa ctttaattaa aaaaactagc taaataacat atataataat
     2581 taagtttatt tttcaaagct tgaagggtat atagtttgaa tataaaaaat atgagctttt
     2641 agctcgacta tccatacagt cgactctaga ggatccccgg gtaccgagct cgaattcatc
     2701 gatgatatca gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt
     2761 taacctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2821 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2881 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2941 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3001 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaagtg
     3061 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3121 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3181 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3241 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3301 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3361 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3421 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     3481 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3541 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3601 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3661 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat ggagttttaa
     3721 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatc
//
