LOCUS       40924_25981       13053 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJC8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Lam KN, Hall MW, Engel K, Vey G, Cheng J, Neufeld JD, Charles TC.
  TITLE     Evaluation of a pooled strategy for high-throughput sequencing of 
            cosmid clones from metagenomic libraries
  JOURNAL   PLoS ONE 9 (6), E98968 (2014)
  PUBMED    24911009
REFERENCE   2  (bases 1 to 13053)
  AUTHORS   Cheng J, Charles TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-NOV-2012) Biology, University of Waterloo, 200 
            University Avenue West, Waterloo, Ontario N2L 3G1, Canada
REFERENCE   3  (bases 1 to 13053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2014"; volume: "9"; issue: "6"; pages: "E98968"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-NOV-2012) Biology, University of Waterloo, 200 University Avenue
            West, Waterloo, Ontario N2L 3G1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    282..381
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(498..1262)
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     protein_bind    complement(1455..1554)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1573..1589)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1597..1613)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1621..1651)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1666..1687)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(1922..2290)
                     /label=traJ
                     /note="oriT-recognizing protein"
     oriT            complement(2323..2432)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3116..3763)
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
     CDS             3869..5065
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_recomb     5436..5668
                     /note="cos; lambda cos site"
     CDS             complement(7470..8615)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             complement(8664..9014)
                     /codon_start=1
                     /gene="ssB"
                     /product="single-stranded DNA binding protein"
                     /label=ssB
                     /protein_id="AGA63627.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(8664..9014)
                     /gene="ssB"
                     /label=ssB
     CDS             9189..9500
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulation of tra gene expression"
                     /protein_id="AGA63628.1"
                     /translation="MTKHELSERAGVSISFLSDLTNGKANPSLKVMEAIADALETPLPL
                     LLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAFIVKKWGDDTRKKLRGRL"
     gene            9189..9500
                     /gene="trbA"
                     /label=trbA
     CDS             complement(10538..11632)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AGA63629.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(10538..11632)
                     /gene="incC"
                     /label=incC
     CDS             complement(11314..11619)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulation of gene expression"
                     /protein_id="AGA63624.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(11314..11619)
                     /gene="korA"
                     /label=korA
     rep_origin      complement(12084..12795)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
       61 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gccattcgcc
      121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      241 gtcacgacgt tgtaaaacga cggccagtga attccagatc tcaaataatg attttatttt
      301 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
      361 ctttgtacaa aaaagcaggc tggatccacg tgcgtgttgc cccagcaatc agcgcgacct
      421 tgcccctcca acgtcatctc gttctccgct catgagctca gccaatcgac tggcgagcgg
      481 catcgcattc ttcgcatccc gccctctggc ggatgcagga agatcaacgg atctcggccc
      541 agttgaccca gggctgtcgc cacaatgtcg cgggagcgga tcaaccgagc aaaggcatga
      601 ccgactggac cttccttctg aaggctcttc tccttgagcc acctgtccgc caaggcaaag
      661 cgctcacagc agtggtcatt ctcgagataa tcgacgcgta ccaacttgcc atcctgaaga
      721 atggtgcagt gtctcggcac cccataggga acctttgcca tcaactcggc aagatgcagc
      781 gtcgtgttgg catcgtgtcc cacgccgagg agaagtacct gcccatcgag ttcatggaca
      841 cgggcgaccg ggcttgcagg cgagtgaggt ggcaggggca atggatcaga gatgatctgc
      901 tctgcctgtg gccccgctgc cgcaaaggca aatggatggg cgctgcgctt tacatttggc
      961 aggcgccaga atgtgtcaga gacaactcca aggtccggtg taacgggcga cgtggcagga
     1021 tcgaacggct cgtcgtccag acctgaccac gagggcatga cgagcgtccc tcccggaccc
     1081 agcgcagcac gcagggcctc gatcagtcca agtggcccat cttcgagggg ccggacgcta
     1141 cggaaggagc tgtggaccag cagcacaccg ccgggggtaa ccccaaggtt gagaagctga
     1201 ccgatgagct cggcttttcg ccattcgtat tgcacgacat tgcactccac cgctgatgac
     1261 atcagtcgat catagcacga tcaacggcac tgttgcaaat agtcggtggt gataaactta
     1321 tcatcccctt ttgctgatgg agctgcacat gaacccattc aaaggccggc attttcagcg
     1381 tgacatcatt ctgtgggccg tacgctggta ctgcaaatac ggcatcagtt accgtgagcc
     1441 ggaggatcca cgtgacccag ctttcttgta caaagttggc attataagaa agcattgctt
     1501 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgaagctt
     1561 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     1621 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     1681 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     1741 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcggtctt
     1801 gccttgctcg tcggtgatgt acttcaccag ctccgcgaag tcgctcttct tgatggagcg
     1861 catggggacg tgcttggcaa tcacgcgcac cccccggccg ttttagcggc taaaaaagtc
     1921 atggctctgc cctcgggcgg accacgccca tcatgacctt gccaagctcg tcctgcttct
     1981 cttcgatctt cgccagcagg gcgaggatcg tggcatcacc gaaccgcgcc gtgcgcgggt
     2041 cgtcggtgag ccagagtttc agcaggccgc ccaggcggcc caggtcgcca ttgatgcggg
     2101 ccagctcgcg gacgtgctca tagtccacga cgcccgtgat tttgtagccc tggccgacgg
     2161 ccagcaggta ggccgacagg ctcatgccgg ccgccgccgc cttttcctca atcgctcttc
     2221 gttcgtctgg aaggcagtac accttgatag gtgggctgcc cttcctggtt ggcttggttt
     2281 catcagccat ccgcttgccc tcatctgtta cgccggcggt agccggccag cctcgcagag
     2341 caggattccc gttgagcacc gccaggtgcg aataagggac agtgaagaag gaacacccgc
     2401 tcgcgggtgg gcctacttca cctatcctgc ccggctgacg ccgttggata caccaaggaa
     2461 agtctacacg aaccctttgg caaaatcctg tatatcgtgc gaaaaaggat ggatataccg
     2521 aaaaaatcgc tataatgacc ccgaagcagg gttatgcagc ggaaaagcgc cacgcttccc
     2581 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2641 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2701 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2761 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2821 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2881 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2941 cagaaggccg ccagagaggc cgagcgcggc cgtgaggctt ggacgctagg gcagggcatg
     3001 aaaaagcccg tagcgggctg ctacgggcgt ctgacgcggt ggaaaggggg aggggatgtt
     3061 gtctacatgg ctctgctgta gtgagtgggt tgcgctccgg cagcggtcct gatcaatcgt
     3121 caccctttct cggtccttca acgttcctga caacgagcct ccttttcgcc aatccatcga
     3181 caatcaccgc gagtccctgc tcgaacgctg cgtccggacc ggcttcgtcg aaggcgtcta
     3241 tcgcggcccg caacagcggc gagagcggag cctgttcaac ggtgccgccg cgctcgccgg
     3301 catcgctgtc gccggcctgc tcctcaagca cggccccaac agtgaagtag ctgattgtca
     3361 tcagcgcatt gacggcgtcc ccggccgaaa aacccgcctc gcagaggaag cgaagctgcg
     3421 cgtcggccgt ttccatctgc ggtgcgcccg gtcgcgtgcc ggcatggatg cgcgcgccat
     3481 cgcggtaggc gagcagcgcc tgcctgaagc tgcgggcatt cccgatcaga aatgagcgcc
     3541 agtcgtcgtc ggctctcggc accgaatgcg tatgattctc cgccagcatg gcttcggcca
     3601 gtgcgtcgag cagcgcccgc ttgttcctga agtgccagta aagcgccggc tgctgaaccc
     3661 ccaaccgttc cgccagtttg cgtgtcgtca gaccgtctac gccgacctcg ttcaacaggt
     3721 ccagggcggc acggatcact gtattcggct gcaactttgt catgcttgac actttatcac
     3781 tgataaacat aatatgtcca ccaacttatc agtgataaag aatccgcgcg ttcaatcgga
     3841 ccagcggagg ctggtccgga ggccagacat gaaacccaac atacccctga tcgtaattct
     3901 gagcactgtc gcgctcgacg ctgtcggcat cggcctgatt atgccggtgc tgccgggcct
     3961 cctgcgcgat ctggttcact cgaacgacgt caccgcccac tatggcattc tgctggcgct
     4021 gtatgcgttg gtgcaatttg cctgcgcacc tgtgctgggc gcgctgtcgg atcgtttcgg
     4081 gcggcggcca atcttgctcg tctcgctggc cggcgccact gtcgactacg ccatcatggc
     4141 gacagcgcct ttcctttggg ttctctatat cgggcggatc gtggccggca tcaccggggc
     4201 gactggggcg gtagccggcg cttatattgc cgatatcact gatggcgatg agcgcgcgcg
     4261 gcacttcggc ttcatgagcg cctgtttcgg gttcgggatg gtcgcgggac ctgtgctcgg
     4321 tgggctgatg ggcggtttct ccccccacgc tccgttcttc gccgcggcag ccttgaacgg
     4381 cctcaatttc ctgacgggct gtttcctttt gccggagtcg cacaaaggcg aacgccggcc
     4441 gttacgccgg gaggctctca acccgctcgc ttcgttccgg tgggcccggg gcatgaccgt
     4501 cgtcgccgcc ctgatggcgg tcttcttcat catgcaactt gtcggacagg tgccggccgc
     4561 gctttgggtc attttcggcg aggatcgctt tcactgggac gcgaccacga tcggcatttc
     4621 gcttgccgca tttggcattc tgcattcact cgcccaggca atgatcaccg gccctgtagc
     4681 cgcccggctc ggcgaaaggc gggcactcat gctcggaatg attgccgacg gcacaggcta
     4741 catcctgctt gccttcgcga cacggggatg gatggcgttc ccgatcatgg tcctgcttgc
     4801 ttcgggtggc atcggaatgc cggcgctgca agcaatgttg tccaggcagg tggatgagga
     4861 acgtcagggg cagctgcaag gctcactggc ggcgctcacc agcctgacct cgatcgtcgg
     4921 acccctcctc ttcacggcga tctatgcggc ttctataaca acgtggaacg ggtgggcatg
     4981 gattgcaggc gctgccctct acttgctctg cctgccggcg ctgcgtcgcg ggctttggag
     5041 cggcgcaggg caacgagccg atcgctgatc gtggaaacga taggcctatg ccatgcgggt
     5101 caaggcgact tccggcaagc tatacgcgcc ctaggagtgc ggttggaacg ttggcccagc
     5161 cagatactcc cgatcacgag caggacgccg atgatttgaa gcgcactcag cgtctgatct
     5221 gcctcgctgg cctgccgcag ttcttcaacc tcccggcgca gcttttcgtt ctcaatttca
     5281 gcatcccttt cggcatacca ttttatgacg gcggcagagt cataaagcac ctcattaccc
     5341 ttgccaccgc ctcgcagaac gggcattccc tgttcctgcc agttctgaat ggtacggata
     5401 ctcgcaccga aaatgtcagc cagctgcttt ttgttgactt ccattgttca ttccacggac
     5461 aaaaacagag aaaggaaacg acagaggcca aaaagctcgc tttcagcacc tgtcgtttcc
     5521 tttcttttca gagggtattt taaataaaaa cattaagtta tgacgaagaa gaacggaaac
     5581 gccttaaacc ggaaaatttt cataaatagc gaaaacccgc gaggtcgccg ccccgtaaca
     5641 aggcggatcg ccggaaagga cccgcaaatg ataataatta tcaattgcat actatcgacg
     5701 gcactgctgc cagataacac caccggggaa acattccatc atgatggccg tgcggacata
     5761 ggaagccagt tcatccatcg ctttcttgtc tgctgccatt tgctttgtga catccagcgc
     5821 cgcacattca gcagcgtttt tcagcgcgtt ttcgatcaac gtttcaatgt tggtatcaac
     5881 accaggttta actttgaact tatcggcact gacggttacc ttgttctgcg ctggctcatc
     5941 acgctggata ccaaggctga tgttgtagat attggtcacc ggctgaggtg tttcgattgc
     6001 cgctgcgtgg atagcaccat ttgcgatagc ggcgtccttg atgaatgaca ctccattgcg
     6061 aataagttcg aaggagacgg tgtcacgaat gcgctggtcc agctcgtcga ttgccttttg
     6121 tgcagcagag gtatcaatct caacgccaag cgtcatcgaa gcgcaatatt gctgctcacc
     6181 aaaacgcgta ttgaccaggt gttcaacggc aaatttctgc ccttctgatg tcagaaaggt
     6241 aaagtgattt tctttctggt attcagttgc tgtgtgtctg gtttcagcaa aaccaagctc
     6301 gcgcaattcg gctgtgccag atttagaagg cagatcacca gacagcaacg cgccacggaa
     6361 aaacagcgca tacagaacat ccgtcgccgc gccggacaac gtgataattt tatgacccat
     6421 gatttatttc cttttagacg tgagcctgtc gcacagcaaa gccgccgaaa gttaacggtt
     6481 tgcccaggct cacaactgaa agactttcta cggtgtgcgc gtgcgatgcg cgtagaagac
     6541 tgatttatca acctgtcttt atatcaggat tcattacctg actatttgtg ggtaaagttc
     6601 gtagtgcgct gatcgtgcaa aatgatttta gttgggaaca gttcgcaact ctgtcccata
     6661 aaaatcagca tattcccatc tatcccatat ccagcgcatt gaccatcggg atactgaagg
     6721 gagattccat catctcttag aaagatcacc atctcttttg tttcaatttg catatagcta
     6781 cctggaggat ttatgaatac aaggattttc atggactatt accatgagat tgattttcca
     6841 tctttattcg cgagagcagt ggaaagcgat gacgatgtgg gtactacatt gcgcattcac
     6901 ctactttgtg agcgcatggt cgaagcatgg atatgcgcat gctgtgactg ccaagatcat
     6961 cacgagcaat aaacccgccg gcaacgcccg cagcagcata ccggcgaccc ctcggcctcg
     7021 ctgttcgggc tccacgaaaa cgccggacag atgcgccttg tgagcgtcct tggggccgtc
     7081 ctcctgtttg aagaccgaca gcccaatgat ctcgccgtcg atgtaggcgc cgaatgccac
     7141 ggcatctcgc aaccgttcag cgaacgcctc catgggcttt ttctcctcgt gctcgtaaac
     7201 ggacccgaac atctctggag ctttcttcag ggccgacaat cggatctcgc ggaaatcctg
     7261 cacgtcggcc gctccaagcc gtcgaatctg agccttaatc acaattgtca attttaatcc
     7321 tctgtttatc ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa
     7381 gtgcgtcgag cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc
     7441 ccagccggaa ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc
     7501 caggcgtgtt ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg
     7561 cgccacttct tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg
     7621 agcgggtacg gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac
     7681 agcttgcggt acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg
     7741 atttcctcgt cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg
     7801 aagcggtgca gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc
     7861 gccgtcgcct gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc
     7921 gaccagccca ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg
     7981 gtgcgcttcg cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc
     8041 tcgacgccgg tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc
     8101 agcgcctcgc gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg
     8161 tttggcatcg ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt
     8221 tccttgatct gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt
     8281 tttgccaggt cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg
     8341 atggtcatcg acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg
     8401 gcggccgatg gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg
     8461 gccgtagctt gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg
     8521 acggtgcggc ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct
     8581 tgcctgtatg ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg
     8641 actcacgccg gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc
     8701 agataatcca ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg
     8761 tacttggtat tccgaatctt gccctgcacg aataccagcg accccttgcc caaatacttg
     8821 ccgtgggcct cggcctgaga gccaaaacac ttgatgcgga agaagtcggt gcgctcctgc
     8881 ttgtcgccgg catcgttgcg ccactcttca ttaaccgcta tatcgaaaat tgcttgcggc
     8941 ttgttagaat tgccatgacg tacctcggtg tcacgggtaa gattaccgat aaactggaac
     9001 tgattatggc tcatatcgaa agtctccttg agaaaggaga ctctagttta gctaaacatt
     9061 ggttccgctg tcaagaactt tagcggctaa aattttgcgg gccgcgacca aaggtgcgag
     9121 gggcggcttc cgctgtgtac aaccagatat ttttcaccaa catccttcgt ctgctcgatg
     9181 agcggggcat gacgaaacat gagctgtcgg agagggcagg ggtttcaatt tcgtttttat
     9241 cagacttaac caacggtaag gccaacccct cgttgaaggt gatggaggcc attgccgacg
     9301 ccctggaaac tcccctacct cttctcctgg agtccaccga ccttgaccgc gaggcactcg
     9361 cggagattgc gggtcatcct ttcaagagca gcgtgccgcc cggatacgaa cgcatcagtg
     9421 tggttttgcc gtcacataag gcgtttatcg taaagaaatg gggcgacgac acccgaaaaa
     9481 agctgcgtgg aaggctctga cgccaagggt tagggcttgc acttccttct ttagccgcta
     9541 aaacggcccc ttctctgcgg gccgtcggct cgcgcatcat atcgacatcc tcaacggaag
     9601 ccgtgccgcg aatggcatcg ggcgggtgcg ctttgacagt tgttttctat cagaacccct
     9661 acgtcgtgcg gttcgattag ctgtttgtct tgcaggctaa acactttcgg tatatcgttt
     9721 gcctgtgcga taatgttgct aatgatttgt tgcgtagggg ttactgaaaa gtgagcggga
     9781 aagaagagtt tcagaccatc aaggagcggg ccaagcgcaa gctggaacgc gacatgggtg
     9841 cggacctgtt ggccgcgctc aacgacccga aaaccgttga agtcatgctc aacgcggacg
     9901 gcaaggtgtg gcacgaacgc cttggcgagc cgatgcggta catctgcgac atgcggccca
     9961 gccagtcgca ggcgattata gaaacggtgg ccggattcca cggcaaagag gtcacgcggc
    10021 attcgcccat cctggaaggc gagttcccct tggatggcag ccgctttgcc ggccaattgc
    10081 cgccggtcgt ggccgcgcca acctttgcga tccgcaagcg cgcggtcgcc atcttcacgc
    10141 tggaacagta cgtcgaggcg ggcatcatga cccgcgagca atacgaggtc attaaaagcg
    10201 ccgtgattga tgatatagcg gcccggctgc tcctggttct cgcgcaccga aatgggtgac
    10261 ttcaccccgc gctctttgat cgtggcaccg atttccgcga tgctctccgg ggaaaagccg
    10321 gggttgtcgg ccgtccgcgg ctgatgcgga tcttcgtcga tcaggtccag gtccagctcg
    10381 atagggccgg aaccgccctg agacgccgca ggagcgtcca ggaggctcga caggtcgccg
    10441 atgctatcca accccaggcc ggacggctgc gccgcgcctg cggcttcctg agcggccgca
    10501 gcggtgtttt tcttggtggt cttggcttga gccgcagtca ttgggaaatc tccatcttcg
    10561 tgaacacgta atcagccagg gcgcgaacct ctttcgatgc cttgcgcgcg gccgttttct
    10621 tgatcttcca gaccggcaca ccggatgcga gggcatcggc gatgctgctg cgcaggccaa
    10681 cggtggccgg aatcatcatc ttggggtacg cggccagcag ctcggcttgg tggcgcgcgt
    10741 ggcgcggatt ccgcgcatcg accttgctgg gcaccatgcc aaggaattgc agcttggcgt
    10801 tcttctggcg cacgttcgca atggtcgtga ccatcttctt gatgccctgg atgctgtacg
    10861 cctcaagctc gatgggggac agcacatagt cggccgcgaa gagggcggcc gccaggccga
    10921 cgccaagggt cggggccgtg tcgatcaggc acacgtcgaa gccttggttc gccagggcct
    10981 tgatgttcgc cccgaacagc tcgcgggcgt cgtccagcga cagccgttcg gcgttcgcca
    11041 gtaccgggtt ggactcgatg agggcgaggc gcgcggcctg gccgtcgccg gctgcgggtg
    11101 cggtttcggt ccagccgccg gcagggacag cgccgaacag cttgcttgca tgcaggccgg
    11161 tagcaaagtc cttgagcgtg taggacgcat tgccctgggg gtccaggtcg atcacggcaa
    11221 cccgcaagcc gcgctcgaaa aagtcgaagg caagatgcac aagggtcgaa gtcttgccga
    11281 cgccgccttt ctggttggcc gtgaccaaag ttttcatcgt ttggtttcct gttttttctt
    11341 ggcgtccgct tcccacttcc ggacgatgta cgcctgatgt tccggcagaa ccgccgttac
    11401 ccgcgcgtac ccctcgggca agttcttgtc ctcgaacgcg gcccacacgc gatgcaccgc
    11461 ttgcgacact gcgcccctgg tcagtcccag cgacgttgcg aacgtcgcct gtggcttccc
    11521 atcgactaag acgccccgcg ctatctcgat ggtctgctgc cccacttcca gcccctggat
    11581 cgcctcctgg aactggcttt cggtaagccg tttcttcatg gataacaccc ataatttgct
    11641 ccgcgccttg gttgaacata gcggtgacag ccgccagcac atgagagaag tttagctaaa
    11701 catttctcgc acgtcaacac ctttagccgc taaaactcgt ccttggcgta acaaaacaaa
    11761 agcccggaaa ccgggctttc gtctcttgcc gcttatggct ctgcacccgg ctccatcacc
    11821 aacaggtcgc gcacgcgctt cactcggttg cggatcgaca ctgccagccc aacaaagccg
    11881 gttgccgccg ccgccaggat cgcgccgatg atgccggcca caccggccat cgcccaccag
    11941 gtcgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca cctgcggcac
    12001 gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg ccccgatccc
    12061 ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg
    12121 gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg
    12181 gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa
    12241 ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg
    12301 ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata
    12361 ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc
    12421 tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc
    12481 acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc
    12541 agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg
    12601 agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg
    12661 cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg
    12721 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccggtga
    12781 gcgtcggaaa ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12841 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12901 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12961 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    13021 cgctcaagtc agaggtggcg aaacccgaca gga
//
