LOCUS       40924_25976       13804 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJC24, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13804)
  AUTHORS   Cheng J, Charles TC.
  TITLE     Cosmids for functional metagenomics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13804)
  AUTHORS   Cheng J, Charles TC.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-DEC-2012) Department of Biology, University of 
            Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, 
            Canada
REFERENCE   3  (bases 1 to 13804)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13804)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-DEC-2012) Department of Biology, University of Waterloo, 200 
            University Avenue West, Waterloo, Ontario N2L 3G1, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     252..268
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    282..381
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(405..1823)
                     /codon_start=1
                     /label=SacB
                     /note="secreted levansucrase that renders bacterial growth 
                     sensitive to sucrose"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
     protein_bind    complement(2206..2305)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2324..2340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2348..2364)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2372..2402)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2417..2438)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(2673..3041)
                     /codon_start=1
                     /label=traJ
                     /note="oriT-recognizing protein"
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(3074..3183)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     CDS             complement(3867..4514)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     CDS             4620..5816
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY
                     GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI
                     VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF
                     FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM
                     QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM
                     LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL
                     AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR
                     "
     misc_recomb     6187..6419
                     /label=lambda cos site
                     /note="lambda cos site"
     CDS             complement(8221..9366)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9415..9765)
                     /codon_start=1
                     /gene="ssB"
                     /product="single-stranded DNA binding protein"
                     /label=ssB
                     /protein_id="AGF38333.1"
                     /translation="MSHNQFQFIGNLTRDTEVRHGNSNKPQAIFDIAVNEEWRNDAGDK
                     QERTDFFRIKCFGSQAEAHGKYLGKGSLVFVQGKIRNTKYEKDGQTVYGTDFIADKVDY
                     LDTKAPGGSNQE"
     gene            complement(9415..9765)
                     /gene="ssB"
                     /label=ssB
     CDS             9940..10251
                     /codon_start=1
                     /gene="trbA"
                     /product="TrbA"
                     /label=trbA
                     /note="regulatory protein"
                     /protein_id="AGF38334.1"
                     /translation="MTKHELSERAGVSISFLSDLTNGKANPSLKVMEAIADALETPLPL
                     LLESTDLDREALAEIAGHPFKSSVPPGYERISVVLPSHKAFIVKKWGDDTRKKLRGRL"
     gene            9940..10251
                     /gene="trbA"
                     /label=trbA
     CDS             complement(11289..12383)
                     /codon_start=1
                     /gene="incC"
                     /product="plasmid maintenance protein"
                     /label=incC
                     /protein_id="AGF38336.1"
                     /translation="MGVIHEETAYRKPVPGGDPGAGSGAADHRDSAGRLSRWEATGDVR
                     NVAGTDQGRSVASGASRVGRVRGQELARGVRAGNGGSAGTSGVHRPEVGSGRQEKTGNQ
                     TMKTLVTANQKGGVGKTSTLVHLAFDFFERGLRVAVIDLDPQGNASYTLKDFATGLHAS
                     KLFGAVPAGGWTETAPAAGDGQAARLALIESNPVLANAERLSLDDARELFGANIKALAN
                     QGFDVCLIDTAPTLGVGLAAALFAADYVLSPIELEAYSIQGIKKMVTTIANVRQKNAKL
                     QFLGMVPSKVDARNPRHARHQAELLAAYPKMMIPATVGLRSSIADALASGVPVWKIKKT
                     AARKASKEVRALADYVFTKMEISQ"
     gene            complement(11289..12383)
                     /gene="incC"
                     /label=incC
     CDS             complement(12065..12370)
                     /codon_start=1
                     /gene="korA"
                     /product="KorA"
                     /label=korA
                     /note="regulatory protein"
                     /protein_id="AGF38335.1"
                     /translation="MKKRLTESQFQEAIQGLEVGQQTIEIARGVLVDGKPQATFATSLG
                     LTRGAVSQAVHRVWAAFEDKNLPEGYARVTAVLPEHQAYIVRKWEADAKKKQETKR"
     gene            complement(12065..12370)
                     /gene="korA"
                     /label=korA
     rep_origin      complement(12835..13546)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
       61 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gccattcgcc
      121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      241 gtcacgacgt tgtaaaacga cggccagtga attccagatc tcaaataatg attttatttt
      301 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
      361 ctttgtacaa aaaagcaggc tggatcccac gtgttgcgtt tttatttgtt aactgttaat
      421 tgtccttgtt caaggatgct gtctttgaca acagatgttt tcttgccttt gatgttcagc
      481 aggaagctcg gcgcaaacgt tgattgtttg tctgcgtaga atcctctgtt tgtcatatag
      541 cttgtaatca cgacattgtt tcctttcgct tgaggtacag cgaagtgtga gtaagtaaag
      601 gttacatcgt taggatcaag atccattttt aacacaaggc cagttttgtt cagcggcttg
      661 tatgggccag ttaaagaatt agaaacataa ccaagcatgt aaatatcgtt agacgtaatg
      721 ccgtcaatcg tcatttttga tccgcgggag tcagtgaaca ggtaccattt gccgttcatt
      781 ttaaagacgt tcgcgcgttc aatttcatct gttactgtgt tagatgcaat cagcggtttc
      841 atcacttttt tcagtgtgta atcatcgttt agctcaatca taccgagagc gccgtttgct
      901 aactcagccg tgcgtttttt atcgctttgc agaagttttt gactttcttg acggaagaat
      961 gatgtgcttt tgccatagta tgctttgtta aataaagatt cttcgccttg gtagccatct
     1021 tcagttccag tgtttgcttc aaatactaag tatttgtggc ctttatcttc tacgtagtga
     1081 ggatctctca gcgtatggtt gtcgcctgag ctgtagttgc cttcatcgat gaactgctgt
     1141 acattttgat acgtttttcc gtcaccgtca aagattgatt tataatcctc tacaccgttg
     1201 atgttcaaag agctgtctga tgctgatacg ttaacttgtg cagttgtcag tgtttgtttg
     1261 ccgtaatgtt taccggagaa atcagtgtag aataaacgga tttttccgtc agatgtaaat
     1321 gtggctgaac ctgaccattc ttgtgtttgg tcttttagga tagaatcatt tgcatcgaat
     1381 ttgtcgctgt ctttaaagac gcggccagcg tttttccagc tgtcaataga agtttcgccg
     1441 actttttgat agaacatgta aatcgatgtg tcatccgcat ttttaggatc tccggctaat
     1501 gcaaagacga tgtggtagcc gtgatagttt gcgacagtgc cgtcagcgtt ttgtaatggc
     1561 cagctgtccc aaacgtccag gccttttgca gaagagatat ttttaattgt ggacgaatca
     1621 aattcagaaa cttgatattt ttcatttttt tgctgttcag ggatttgcag catatcatgg
     1681 cgtgtaatat gggaaatgcc gtatgtttcc ttatatggct tttggttcgt ttctttcgca
     1741 aacgcttgag ttgcgcctcc tgccagcagt gcggtagtaa aggttaatac tgttgcttgt
     1801 tttgcaaact ttttgatgtt catcgttcat gtctcctttt ttatgtactg tgttagcggt
     1861 ctgcttcttc cagccctcct gtttgaagat ggcaagttag ttacgcacaa taaaaaaaga
     1921 cctaaaatat gtaaggggtg acgccaaagt atacactttg ccctttacac attttaggtc
     1981 ttgcctgctt tatcagtaac aaacccgcgc gatttacttt tcgacctcat tctattagac
     2041 tctcgtttgg attgcaactg gtctattttc ctcttttgtt tgatagaaaa tcataaaagg
     2101 atttgcagac tacgggccta aagaactaaa aaatctatct gtttcttttc attctctgta
     2161 ttttttatag tttctgttgc atgggcataa agttggatcc acgtgaccca gctttcttgt
     2221 acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta
     2281 tcagtcaaaa taaaatcatt atttgaagct tggcgtaatc atggtcatag ctgtttcctg
     2341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2521 gaggcggttt gcgtattggg cgctcggtct tgccttgctc gtcggtgatg tacttcacca
     2581 gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca atcacgcgca
     2641 ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg gaccacgccc
     2701 atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag ggcgaggatc
     2761 gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt cagcaggccg
     2821 cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc atagtccacg
     2881 acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag gctcatgccg
     2941 gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta caccttgata
     3001 ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc ctcatctgtt
     3061 acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac cgccaggtgc
     3121 gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc acctatcctg
     3181 cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg gcaaaatcct
     3241 gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac cccgaagcag
     3301 ggttatgcag cggaaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3361 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3421 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3481 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3541 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3601 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3661 cgagtcagtg agcgaggaag cggaagagcg ccagaaggcc gccagagagg ccgagcgcgg
     3721 ccgtgaggct tggacgctag ggcagggcat gaaaaagccc gtagcgggct gctacgggcg
     3781 tctgacgcgg tggaaagggg gaggggatgt tgtctacatg gctctgctgt agtgagtggg
     3841 ttgcgctccg gcagcggtcc tgatcaatcg tcaccctttc tcggtccttc aacgttcctg
     3901 acaacgagcc tccttttcgc caatccatcg acaatcaccg cgagtccctg ctcgaacgct
     3961 gcgtccggac cggcttcgtc gaaggcgtct atcgcggccc gcaacagcgg cgagagcgga
     4021 gcctgttcaa cggtgccgcc gcgctcgccg gcatcgctgt cgccggcctg ctcctcaagc
     4081 acggccccaa cagtgaagta gctgattgtc atcagcgcat tgacggcgtc cccggccgaa
     4141 aaacccgcct cgcagaggaa gcgaagctgc gcgtcggccg tttccatctg cggtgcgccc
     4201 ggtcgcgtgc cggcatggat gcgcgcgcca tcgcggtagg cgagcagcgc ctgcctgaag
     4261 ctgcgggcat tcccgatcag aaatgagcgc cagtcgtcgt cggctctcgg caccgaatgc
     4321 gtatgattct ccgccagcat ggcttcggcc agtgcgtcga gcagcgcccg cttgttcctg
     4381 aagtgccagt aaagcgccgg ctgctgaacc cccaaccgtt ccgccagttt gcgtgtcgtc
     4441 agaccgtcta cgccgacctc gttcaacagg tccagggcgg cacggatcac tgtattcggc
     4501 tgcaactttg tcatgcttga cactttatca ctgataaaca taatatgtcc accaacttat
     4561 cagtgataaa gaatccgcgc gttcaatcgg accagcggag gctggtccgg aggccagaca
     4621 tgaaacccaa catacccctg atcgtaattc tgagcactgt cgcgctcgac gctgtcggca
     4681 tcggcctgat tatgccggtg ctgccgggcc tcctgcgcga tctggttcac tcgaacgacg
     4741 tcaccgccca ctatggcatt ctgctggcgc tgtatgcgtt ggtgcaattt gcctgcgcac
     4801 ctgtgctggg cgcgctgtcg gatcgtttcg ggcggcggcc aatcttgctc gtctcgctgg
     4861 ccggcgccac tgtcgactac gccatcatgg cgacagcgcc tttcctttgg gttctctata
     4921 tcgggcggat cgtggccggc atcaccgggg cgactggggc ggtagccggc gcttatattg
     4981 ccgatatcac tgatggcgat gagcgcgcgc ggcacttcgg cttcatgagc gcctgtttcg
     5041 ggttcgggat ggtcgcggga cctgtgctcg gtgggctgat gggcggtttc tccccccacg
     5101 ctccgttctt cgccgcggca gccttgaacg gcctcaattt cctgacgggc tgtttccttt
     5161 tgccggagtc gcacaaaggc gaacgccggc cgttacgccg ggaggctctc aacccgctcg
     5221 cttcgttccg gtgggcccgg ggcatgaccg tcgtcgccgc cctgatggcg gtcttcttca
     5281 tcatgcaact tgtcggacag gtgccggccg cgctttgggt cattttcggc gaggatcgct
     5341 ttcactggga cgcgaccacg atcggcattt cgcttgccgc atttggcatt ctgcattcac
     5401 tcgcccaggc aatgatcacc ggccctgtag ccgcccggct cggcgaaagg cgggcactca
     5461 tgctcggaat gattgccgac ggcacaggct acatcctgct tgccttcgcg acacggggat
     5521 ggatggcgtt cccgatcatg gtcctgcttg cttcgggtgg catcggaatg ccggcgctgc
     5581 aagcaatgtt gtccaggcag gtggatgagg aacgtcaggg gcagctgcaa ggctcactgg
     5641 cggcgctcac cagcctgacc tcgatcgtcg gacccctcct cttcacggcg atctatgcgg
     5701 cttctataac aacgtggaac gggtgggcat ggattgcagg cgctgccctc tacttgctct
     5761 gcctgccggc gctgcgtcgc gggctttgga gcggcgcagg gcaacgagcc gatcgctgat
     5821 cgtggaaacg ataggcctat gccatgcggg tcaaggcgac ttccggcaag ctatacgcgc
     5881 cctaggagtg cggttggaac gttggcccag ccagatactc ccgatcacga gcaggacgcc
     5941 gatgatttga agcgcactca gcgtctgatc tgcctcgctg gcctgccgca gttcttcaac
     6001 ctcccggcgc agcttttcgt tctcaatttc agcatccctt tcggcatacc attttatgac
     6061 ggcggcagag tcataaagca cctcattacc cttgccaccg cctcgcagaa cgggcattcc
     6121 ctgttcctgc cagttctgaa tggtacggat actcgcaccg aaaatgtcag ccagctgctt
     6181 tttgttgact tccattgttc attccacgga caaaaacaga gaaaggaaac gacagaggcc
     6241 aaaaagctcg ctttcagcac ctgtcgtttc ctttcttttc agagggtatt ttaaataaaa
     6301 acattaagtt atgacgaaga agaacggaaa cgccttaaac cggaaaattt tcataaatag
     6361 cgaaaacccg cgaggtcgcc gccccgtaac aaggcggatc gccggaaagg acccgcaaat
     6421 gataataatt atcaattgca tactatcgac ggcactgctg ccagataaca ccaccgggga
     6481 aacattccat catgatggcc gtgcggacat aggaagccag ttcatccatc gctttcttgt
     6541 ctgctgccat ttgctttgtg acatccagcg ccgcacattc agcagcgttt ttcagcgcgt
     6601 tttcgatcaa cgtttcaatg ttggtatcaa caccaggttt aactttgaac ttatcggcac
     6661 tgacggttac cttgttctgc gctggctcat cacgctggat accaaggctg atgttgtaga
     6721 tattggtcac cggctgaggt gtttcgattg ccgctgcgtg gatagcacca tttgcgatag
     6781 cggcgtcctt gatgaatgac actccattgc gaataagttc gaaggagacg gtgtcacgaa
     6841 tgcgctggtc cagctcgtcg attgcctttt gtgcagcaga ggtatcaatc tcaacgccaa
     6901 gcgtcatcga agcgcaatat tgctgctcac caaaacgcgt attgaccagg tgttcaacgg
     6961 caaatttctg cccttctgat gtcagaaagg taaagtgatt ttctttctgg tattcagttg
     7021 ctgtgtgtct ggtttcagca aaaccaagct cgcgcaattc ggctgtgcca gatttagaag
     7081 gcagatcacc agacagcaac gcgccacgga aaaacagcgc atacagaaca tccgtcgccg
     7141 cgccggacaa cgtgataatt ttatgaccca tgatttattt ccttttagac gtgagcctgt
     7201 cgcacagcaa agccgccgaa agttaacggt ttgcccaggc tcacaactga aagactttct
     7261 acggtgtgcg cgtgcgatgc gcgtagaaga ctgatttatc aacctgtctt tatatcagga
     7321 ttcattacct gactatttgt gggtaaagtt cgtagtgcgc tgatcgtgca aaatgatttt
     7381 agttgggaac agttcgcaac tctgtcccat aaaaatcagc atattcccat ctatcccata
     7441 tccagcgcat tgaccatcgg gatactgaag ggagattcca tcatctctta gaaagatcac
     7501 catctctttt gtttcaattt gcatatagct acctggagga tttatgaata caaggatttt
     7561 catggactat taccatgaga ttgattttcc atctttattc gcgagagcag tggaaagcga
     7621 tgacgatgtg ggtactacat tgcgcattca cctactttgt gagcgcatgg tcgaagcatg
     7681 gatatgcgca tgctgtgact gccaagatca tcacgagcaa taaacccgcc ggcaacgccc
     7741 gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca
     7801 gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga
     7861 tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct
     7921 ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca
     7981 gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct
     8041 gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc
     8101 cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg
     8161 aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta
     8221 gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg
     8281 caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga
     8341 tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa
     8401 atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt
     8461 cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg
     8521 ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag
     8581 gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc
     8641 ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta
     8701 gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg
     8761 ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc
     8821 cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg
     8881 cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca
     8941 cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag
     9001 caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg
     9061 cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc
     9121 ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcaggggg
     9181 agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac
     9241 ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc
     9301 ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg
     9361 attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc
     9421 ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc
     9481 ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac
     9541 gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca
     9601 cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccactcttc
     9661 attaaccgct atatcgaaaa ttgcttgcgg cttgttagaa ttgccatgac gtacctcggt
     9721 gtcacgggta agattaccga taaactggaa ctgattatgg ctcatatcga aagtctcctt
     9781 gagaaaggag actctagttt agctaaacat tggttccgct gtcaagaact ttagcggcta
     9841 aaattttgcg ggccgcgacc aaaggtgcga ggggcggctt ccgctgtgta caaccagata
     9901 tttttcacca acatccttcg tctgctcgat gagcggggca tgacgaaaca tgagctgtcg
     9961 gagagggcag gggtttcaat ttcgttttta tcagacttaa ccaacggtaa ggccaacccc
    10021 tcgttgaagg tgatggaggc cattgccgac gccctggaaa ctcccctacc tcttctcctg
    10081 gagtccaccg accttgaccg cgaggcactc gcggagattg cgggtcatcc tttcaagagc
    10141 agcgtgccgc ccggatacga acgcatcagt gtggttttgc cgtcacataa ggcgtttatc
    10201 gtaaagaaat ggggcgacga cacccgaaaa aagctgcgtg gaaggctctg acgccaaggg
    10261 ttagggcttg cacttccttc tttagccgct aaaacggccc cttctctgcg ggccgtcggc
    10321 tcgcgcatca tatcgacatc ctcaacggaa gccgtgccgc gaatggcatc gggcgggtgc
    10381 gctttgacag ttgttttcta tcagaacccc tacgtcgtgc ggttcgatta gctgtttgtc
    10441 ttgcaggcta aacactttcg gtatatcgtt tgcctgtgcg ataatgttgc taatgatttg
    10501 ttgcgtaggg gttactgaaa agtgagcggg aaagaagagt ttcagaccat caaggagcgg
    10561 gccaagcgca agctggaacg cgacatgggt gcggacctgt tggccgcgct caacgacccg
    10621 aaaaccgttg aagtcatgct caacgcggac ggcaaggtgt ggcacgaacg ccttggcgag
    10681 ccgatgcggt acatctgcga catgcggccc agccagtcgc aggcgattat agaaacggtg
    10741 gccggattcc acggcaaaga ggtcacgcgg cattcgccca tcctggaagg cgagttcccc
    10801 ttggatggca gccgctttgc cggccaattg ccgccggtcg tggccgcgcc aacctttgcg
    10861 atccgcaagc gcgcggtcgc catcttcacg ctggaacagt acgtcgaggc gggcatcatg
    10921 acccgcgagc aatacgaggt cattaaaagc gccgtgattg atgatatagc ggcccggctg
    10981 ctcctggttc tcgcgcaccg aaatgggtga cttcaccccg cgctctttga tcgtggcacc
    11041 gatttccgcg atgctctccg gggaaaagcc ggggttgtcg gccgtccgcg gctgatgcgg
    11101 atcttcgtcg atcaggtcca ggtccagctc gatagggccg gaaccgccct gagacgccgc
    11161 aggagcgtcc aggaggctcg acaggtcgcc gatgctatcc aaccccaggc cggacggctg
    11221 cgccgcgcct gcggcttcct gagcggccgc agcggtgttt ttcttggtgg tcttggcttg
    11281 agccgcagtc attgggaaat ctccatcttc gtgaacacgt aatcagccag ggcgcgaacc
    11341 tctttcgatg ccttgcgcgc ggccgttttc ttgatcttcc agaccggcac accggatgcg
    11401 agggcatcgg cgatgctgct gcgcaggcca acggtggccg gaatcatcat cttggggtac
    11461 gcggccagca gctcggcttg gtggcgcgcg tggcgcggat tccgcgcatc gaccttgctg
    11521 ggcaccatgc caaggaattg cagcttggcg ttcttctggc gcacgttcgc aatggtcgtg
    11581 accatcttct tgatgccctg gatgctgtac gcctcaagct cgatggggga cagcacatag
    11641 tcggccgcga agagggcggc cgccaggccg acgccaaggg tcggggccgt gtcgatcagg
    11701 cacacgtcga agccttggtt cgccagggcc ttgatgttcg ccccgaacag ctcgcgggcg
    11761 tcgtccagcg acagccgttc ggcgttcgcc agtaccgggt tggactcgat gagggcgagg
    11821 cgcgcggcct ggccgtcgcc ggctgcgggt gcggtttcgg tccagccgcc ggcagggaca
    11881 gcgccgaaca gcttgcttgc atgcaggccg gtagcaaagt ccttgagcgt gtaggacgca
    11941 ttgccctggg ggtccaggtc gatcacggca acccgcaagc cgcgctcgaa aaagtcgaag
    12001 gcaagatgca caagggtcga agtcttgccg acgccgcctt tctggttggc cgtgaccaaa
    12061 gttttcatcg tttggtttcc tgttttttct tggcgtccgc ttcccacttc cggacgatgt
    12121 acgcctgatg ttccggcaga accgccgtta cccgcgcgta cccctcgggc aagttcttgt
    12181 cctcgaacgc ggcccacacg cgatgcaccg cttgcgacac tgcgcccctg gtcagtccca
    12241 gcgacgttgc gaacgtcgcc tgtggcttcc catcgactaa gacgccccgc gctatctcga
    12301 tggtctgctg ccccacttcc agcccctgga tcgcctcctg gaactggctt tcggtaagcc
    12361 gtttcttcat ggataacacc cataatttgc tccgcgcctt ggttgaacat agcggtgaca
    12421 gccgccagca catgagagaa gtttagctaa acatttctcg cacgtcaaca cctttagccg
    12481 ctaaaactcg tccttggcgt aacaaaacaa aagcccggaa accgggcttt cgtctcttgc
    12541 cgcttatggc tctgcacccg gctccatcac caacaggtcg cgcacgcgct tcactcggtt
    12601 gcggatcgac actgccagcc caacaaagcc ggttgccgcc gccgccagga tcgcgccgat
    12661 gatgccggcc acaccggcca tcgcccacca ggtcgccgcc ctcactgccc ggcacctggt
    12721 cgctgaatgt cgatgccagc acctgcggca cgtcaatgct tccgggcgtc gcgctcgggc
    12781 tgatcgccca tcccgttact gccccgatcc cggcaatggc aaggactgcc agcgctgcca
    12841 tttttggggt gaggccgttc gcggccgagg ggcgcagccc ctggggggat gggaggcccg
    12901 cgttagcggg ccgggagggt tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac
    12961 gcgcacaggg cgcagccctg gttaaaaaca aggtttataa atattggttt aaaagcaggt
    13021 taaaagacag gttagcggtg gccgaaaaac gggcggaaac ccttgcaaat gctggatttt
    13081 ctgcctgtgg acagcccctc aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc
    13141 ccctcaagtg tcaaggatcg cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa
    13201 taccgcaggg cacttatccc caggcttgtc cacatcatct gtgggaaact cgcgtaaaat
    13261 caggcgtttt cgccgatttg cgaggctggc cagctccacg tcgccggccg aaatcgagcc
    13321 tgcccctcat ctgtcaacgc cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc
    13381 cctcatctgt cagtgagggc caagttttcc gcgaggtatc cacaacgccg gcggccgcgg
    13441 tgtctcgcac acggcttcga cggcgtttct ggcgcgtttg cagggccata gacggccgcc
    13501 agcccagcgg cgagggcaac cagcccggtg agcgtcggaa aggcgctctt ccgcttcctc
    13561 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    13621 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    13681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    13741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    13801 agga
//
