LOCUS       V015746                 5486 bp    DNA     circular SYN 10-MAR-2019
DEFINITION  Exported.
ACCESSION   V015746
VERSION     V015746
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Cui J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUN-2018) MCB, Dartmouth College, 14 Engineering
            Drive, Hanover, NH 03755, USA
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (28-JUN-2018) MCB, Dartmouth College, 14 Engineering Drive, Hanover,
            NH 03755, USA"
FEATURES             Location/Qualifiers
     source          1..5486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(100..1047)
                     /label="Rep101"
                     /note="RepA protein needed for replication with the pSC101
                     origin"
     rep_origin      complement(1095..1317)
                     /direction=LEFT
                     /label="pSC101 ori"
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     misc_feature    1844..2328
                     /label="upstream homology recombination flank"
                     /note="upstream homology recombination flank"
     CDS             2329..2499
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /label="ferredoxin"
                     /note="Clo1313_2761"
                     /protein_id="QBI89800.1"
                     /translation="MAYFITDACISCGACESECPVSCISPGDSVYVIDADACIECGACA
                     NVCPVDAPQQK"
     misc_feature    2500..3040
                     /label="downstream homology recombination flank"
                     /note="downstream homology recombination flank"
     regulatory      3045..3575
                     /label="kanamycin promoter"
                     /note="kanamycin promoter"
                     /regulatory_class="promoter"
     gene            3576..4337
                     /gene="htk"
                     /label="htk"
     CDS             3576..4337
                     /codon_start=1
                     /transl_table=11
                     /gene="htk"
                     /product="highly thermostable kanamycin
                     nucleotidyltransferase"
                     /EC_number="2.7.7.46"
                     /label="htk"
                     /protein_id="QBI89801.1"
                     /translation="MKGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGRQTD
                     GPYSDIEMMCVLSTEGVEFSYEWTTGEWKAEVNFYSEEILLDYASRVEPDWPLTHGRFF
                     SILPIYDPGGYFEKVYQTAKSVEAQKFHDAICALIVEELFEYAGKWRNIRVQGPTTFLP
                     SLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQPDLPPGYVQLCQLVMSGQLSDPEK
                     LLESLENFWNGVQEWAERHGYIVDVSKRIPF"
     CDS             4361..4939
                     /gene="tdk"
                     /label="Thymidine kinase"
                     /note="Thymidine kinase from Thermoanaerobacter
                     pseudethanolicus (strain ATCC 33223 / 39E). Accession#:
                     B0K7G9"
     misc_feature    5034..5486
                     /label="gene target internal homology recombination flank"
                     /note="gene target internal homology recombination flank"
ORIGIN
        1 gacagttttc cctttgatat gtaacggtga acagttgttc tacttttgtt tgttagtctt
       61 gatgcttcac tgatagatac aagagccata agaacctcag atccttccgt atttagccag
      121 tatgttctct agtgtggttc gttgtttttg cgtgagccat gagaacgaac cattgagatc
      181 atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa ctggtgagct gaatttttgc
      241 agttaaagca tcgtgtagtg tttttcttag tccgttatgt aggtaggaat ctgatgtaat
      301 ggttgttggt attttgtcac cattcatttt tatctggttg ttctcaagtt cggttacgag
      361 atccatttgt ctatctagtt caacttggaa aatcaacgta tcagtcgggc ggcctcgctt
      421 atcaaccacc aatttcatat tgctgtaagt gtttaaatct ttacttattg gtttcaaaac
      481 ccattggtta agccttttaa actcatggta gttattttca agcattaaca tgaacttaaa
      541 ttcatcaagg ctaatctcta tatttgcctt gtgagttttc ttttgtgtta gttcttttaa
      601 taaccactca taaatcctca tagagtattt gttttcaaaa gacttaacat gttccagatt
      661 atattttatg aattttttta actggaaaag ataaggcaat atctcttcac taaaaactaa
      721 ttctaatttt tcgcttgaga acttggcata gtttgtccac tggaaaatct caaagccttt
      781 aaccaaagga ttcctgattt ccacagttct cgtcatcagc tctctggttg ctttagctaa
      841 tacaccataa gcattttccc tactgatgtt catcatctga gcgtattggt tataagtgaa
      901 cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg gggttgagta gtgccacaca
      961 gcataaaatt agcttggttt catgctccgt taagtcatag cgactaatcg ctagttcatt
     1021 tgctttgaaa acaactaatt cagacataca tctcaattgg tctaggtgat tttaatcact
     1081 ataccaattg agatgggcta gtcaatgata attactagtc cttttccttt gagttgtggg
     1141 tatctgtaaa ttctgctaga cctttgctgg aaaacttgta aattctgcta gaccctctgt
     1201 aaattccgct agacctttgt gtgttttttt tgtttatatt caagtggtta taatttatag
     1261 aataaagaaa gaataaaaaa agataaaaag aatagatccc agccctgtgt ataactcact
     1321 actttagtca gttccgcagt attacaaaag gatgtcgcaa acgctgtttg ctcctctaca
     1381 aaacagacct taaaacccta aaggcttaag tagcaccctc gcaagctcgg gcaaatcgct
     1441 gaatattcct tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc
     1501 agttcgctgc gctcacggct ctggcagtga atgggggtaa atggcactac aggcgccttt
     1561 tatggattca tgcaaggaaa ctacccataa tacaagaaaa gcccgtcacg ggcttctcag
     1621 ggcgttttat ggcgggtctg cggagtggtg aatccgttag cgaggtgccg ccggcttcca
     1681 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt
     1741 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa
     1801 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtctgcagc ttctatacac
     1861 aaatatttaa gcgaaaagtg atttttaact gtaaaacacc atttaattgc gttgtatatg
     1921 gtcaaatgca ataaatggtg tttttgtctc taaaatttga ttaactttaa acgcaaaaag
     1981 caacgctttt aaaagcgttg cttttagaag tagccttaag gggagtcgaa cccctgtctt
     2041 cgccgtgaga gggcgatgtc ctaggccact agacgataag gccaaatatt ggctgccgaa
     2101 ctaggattcg aacctagact agatgatcca gagtcaccgg tgctaccgtt acaccattcg
     2161 gcatcaactg acagaactta ttatagcatg ggtttttatt tttgacaagg gggtgttaaa
     2221 aaatttttat aaaaaactga cttgttttga gtgtttacat attaagttaa atgctatata
     2281 atatcctttg aagttatata ctgtatacat aaaagggggg atatgacaat ggcatatttt
     2341 ataaccgatg cttgcatcag ctgcggtgct tgcgagtctg aatgcccggt atcttgtatt
     2401 tcaccaggag acagcgttta tgtaatagat gctgacgctt gtatagaatg tggagcctgt
     2461 gcaaatgttt gccctgttga tgctcctcag cagaaataag attaacgaaa agtccctttc
     2521 agggggcttt ttgtttaatt gacttaaaaa tttcaaagtg gtataatcta caataatata
     2581 ttttagatag attaggagga gtcataatgc ctatagtttt tcttaatggt gagtttgtag
     2641 acagtgaaaa agcggctgtt tcggtttttg accatggatt tctttatggt gacggtgttt
     2701 ttgaaggtat aagggcgtat gatggtgttg tttttaaatt agatgatcac ttaaagagac
     2761 tttacaatat ggctaaggca cttcttttag atataccata ctctaaagaa gaaatggccg
     2821 ataaagtctt ggaaactgtg aggagaaaca acttaaaaga tgcatacata agattagttg
     2881 tttcaagagg gaaaggagat ttagggctcg atccctacaa atgttcaaag cctacagtag
     2941 ttatcattgc agataagatt acattgtatc ctgatgaaat gtaccaaaat ggattaaaaa
     3001 ttatcacatc tacgtttagg cgcaactcca ttcaaacctt cgtaaaaccc agcgaaccat
     3061 ttgaggtgat aggtaagatt ataccgaggt atgaaaacga gaattggacc tttacagaat
     3121 tactctatga agcgccatat ttaaaaagct accaagacga agaggatgaa gaggatgagg
     3181 aggcagattg ccttgaatat attgacaata ctgataagat aatatatctt ttatatagaa
     3241 gatatcgccg tatgtaagga tttcaggggg caaggcatag gcagcgcgct tatcaatata
     3301 tctatagaat gggcaaagca taaaaacttg catggactaa tgcttgaaac ccaggacaat
     3361 aaccttatag cttgtaaatt ctatcataat tgtggtttca aaatcggctc cgtcgatact
     3421 atgttatacg ccaactttca aaacaacttt gaaaaagctg ttttctggta tttaaggttt
     3481 tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc
     3541 tttaaatact gtagaaaaga ggaaggaaat aataaatgaa aggaccaata ataatgacta
     3601 gagaagaaag aatgaagatt gttcatgaaa ttaaggaacg aatattggat aaatatgggg
     3661 atgatgttaa ggcaattggt gtttatggct ctcttggtcg tcagactgat gggccctatt
     3721 cggatattga gatgatgtgt gttctgtcaa cagagggagt agagttcagc tatgaatgga
     3781 caaccggtga gtggaaggcg gaagtgaatt tttatagcga agagattcta ctagattatg
     3841 catctcgggt ggaaccggat tggccgctta cacatggtcg atttttctct attttgccga
     3901 tttatgatcc aggtggatac tttgagaaag tgtaccaaac tgctaaatcg gtagaagccc
     3961 aaaagttcca cgatgcgatc tgtgccctta tcgtagaaga gctgtttgaa tatgcaggca
     4021 aatggcgtaa tattcgtgtg caaggaccga caacatttct accatccttg actgtacagg
     4081 tggcaatggc aggtgccatg ttgattggtc tgcatcatcg catctgttat acgacgagcg
     4141 cttcggtctt aactgaagca gttaagcaac cagatcttcc tccaggttat gtccaactgt
     4201 gccagctcgt aatgtctggt caactttccg accctgagaa acttctggaa tcgctagaga
     4261 atttctggaa tggggttcag gagtgggcgg aacgacacgg atatatagtg gatgtgtcaa
     4321 aacgcatacc attttgatga cattaagaaa ggtggttttt atgatatacg gttctttgga
     4381 tcatggtttt atagaggcta tagttggtcc tatgtttagc ggtaaaagtg aagaactcat
     4441 aagaaggatt aaaagagccc aaattgccaa acaaaaagta caagtcttta aaccggctat
     4501 tgacgataga tattctattg acaaggtggt atctcacaat ggcactaata taaatgctat
     4561 tagtgtagta aaggcttctg aaataattga gcttttagaa gaagacacag aggttatcgc
     4621 aattgatgaa attcagtttt ttgaccattc gattgtggat gttgtaagag aaattgcaga
     4681 tctaggcaaa agagtcattt gtgctggctt agatatggac tttagggggg aaccttttgg
     4741 ccctactcct gatataatgg caattgcgga gtctgtagat aaactgacgg ctatatgtgt
     4801 gaaatgtggc aatcctgcta ctcgcactca aaggcttatc aatgggaaac ctgcaaaata
     4861 cgatgaccca atcattttag taggggccca tgaaacctat gaagcaaggt gtcgaaaatg
     4921 ccatgaagtt cctcgtacat agtagtacct agatttagat gcctaaaaag ctttaactac
     4981 aagcttttta gacatctaat cttttctgaa gtacatccgc aactgtccat actgctattc
     5041 acgaaggtac aggaaaatac gaagtggatg ctgatacatg tatagattgt ggtgcatgtg
     5101 aaccagtatg cccaacagga gctattaagg ctgaataaga ttaacgaaaa gtccctttca
     5161 gggggctttt tgtttaattg acttaaaaat ttcaaagtgg tataatctac aataatatat
     5221 tttagataga ttaggaggag tcataatgcc tatagttttt cttaatggtg agtttgtaga
     5281 cagtgaaaaa gcggctgttt cggtttttga ccatggattt ctttatggtg acggtgtttt
     5341 tgaaggtata agggcgtatg atggtgttgt ttttaaatta gatgatcact taaagagact
     5401 ttacaatatg gctaaggcac ttcttttaga tataccatac tctaaagaag aaatggccga
     5461 taaagtcttg gaaactgtga ggagaa
//
