LOCUS       40924_25961        8463 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pJBtrc10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8463)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8463)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8463)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8463)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(796..1983)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2031..2059)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(2072..2088)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2699..3844)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      complement(3953..4002)
                     /label=Tn5 neomycin resistance promoter
                     /note="Tn5 neomycin resistance promoter"
                     /regulatory_class="promoter"
     rep_origin      complement(4236..4766)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5249..5326
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5327..6406
                     /label=lacI
                     /note="lac repressor"
     promoter        6641..6670
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    6678..6694
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6713..6735
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     promoter        complement(6797..6814)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      complement(6799..6815)
                     /label=T3 promoter
                     /note="T3 promoter"
                     /regulatory_class="promoter"
     terminator      6833..6880
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     regulatory      6954..7001
                     /label=T7 terminator
                     /note="T7 terminator"
                     /regulatory_class="terminator"
     terminator      6954..7001
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      7073..7121
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     regulatory      7180..7261
                     /label=fd terminator
                     /note="fd terminator"
                     /regulatory_class="terminator"
     terminator      7183..7231
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     promoter        complement(7897..8001)
                     /label=AmpR promoter
     oriT            8178..8286
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     complement(8373..8389)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat
       61 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg
      121 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc
      181 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg
      241 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc
      301 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag
      361 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg
      421 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg
      481 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg
      541 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga
      601 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc
      661 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag
      721 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc
      781 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag
      841 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga
      901 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc
      961 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc
     1021 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat
     1081 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc
     1141 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     1201 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     1261 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     1321 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     1381 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg
     1441 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg
     1501 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac
     1561 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc
     1621 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc
     1681 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc
     1741 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac
     1801 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc
     1861 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc
     1921 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt
     1981 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt
     2041 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact
     2101 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     2161 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     2221 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     2281 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     2341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     2401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     2461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     2641 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc
     2701 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca
     2761 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc
     2821 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat
     2881 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg
     2941 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg
     3001 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt
     3061 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct
     3121 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga
     3181 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc
     3241 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct
     3301 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg
     3361 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg
     3421 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca
     3481 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct
     3541 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct
     3601 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag
     3661 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg
     3721 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct
     3781 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat
     3841 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct
     3901 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     3961 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     4021 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     4081 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4141 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga
     4201 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg
     4261 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca
     4321 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac
     4381 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat
     4441 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat
     4501 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc
     4561 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc
     4621 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt
     4681 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc
     4741 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct
     4801 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactcttaag ctgcaatgat
     5101 accgttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5161 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
     5221 cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg
     5281 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac
     5341 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa
     5401 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct
     5461 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg
     5521 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc
     5581 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga
     5641 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa
     5701 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc
     5761 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga
     5821 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt
     5881 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct
     5941 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg
     6001 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc
     6061 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg
     6121 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc
     6181 gtcaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct
     6241 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa
     6301 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6361 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6421 ttaatgtgag ttagcgcgaa ttgatctggt ttgacagctt atcatcgact gcacggtgca
     6481 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa
     6541 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc
     6601 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc
     6661 tcgtataatg tgtggaattg tgagcggata acaatttcac tctagaaata attttgttta
     6721 actttaagaa ggagatatac atatgggccc ggatccctcg aggtcgacga attcgagctc
     6781 ggccgacttg gccttccctt tagtgagggt taataaactt ggtgagcaat aactagcata
     6841 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg
     6901 cgctcatacg atatgaacgt tgagactgcc gctgagttat cagtgagcaa taactagcat
     6961 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat
     7021 ccggatagct tatcgctaga ggtcgaaatt cacctcgaaa gcaagctgat aaaccgatac
     7081 aattaaaggc tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta
     7141 ttcgcaattc caagctaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc
     7201 tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc
     7261 caagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     7321 ggagacggtc acagcttgtc tgtaagcgga tgcagatcac gcgccctgta gcggcgcatt
     7381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7561 caaaaaactt gattagggtg atggttcaca ctcaaccaag tcattctgag aatagtgtat
     7621 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     7681 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     7741 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     7801 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     7861 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     7921 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     7981 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgatcgacgg
     8041 atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca
     8101 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt
     8161 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc
     8221 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc
     8281 tggccgtaag ctctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8341 cacgaggccc tttcgtcttc aagaattaat tcactggccg tcgttttaca acgtcgtgac
     8401 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8461 aga
//
