LOCUS       V005365                12196 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005365
VERSION     V005365
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 12196)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12196)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    236..257
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        272..302
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    310..326
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     334..350
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(672..3320)
                     /note="T7 RNA polymerase from Escherichia phage T7.
                     Accession#: P00573"
                     /label="T7 RNA polymerase"
     regulatory      complement(3349..3533)
                     /label="LLT"
                     /note="LLT"
                     /regulatory_class="terminator"
     regulatory      complement(3644..3725)
                     /label="fdT"
                     /note="fdT"
                     /regulatory_class="terminator"
     terminator      3674..3722
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     terminator      complement(3901..3949)
                     /label="fd terminator"
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             complement(4599..4664)
                     /label="lambda N peptide"
                     /note="N-terminal RNA-binding domain from the lambda
                     bacteriophage antiterminator protein N (Baron-Benhamou et
                     al., 2004)"
     misc_feature    complement(4816..4886)
                     /label="nutL"
                     /note="nutL"
     protein_bind    4945..4961
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4969..5013)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     promoter        5355..5432
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             5433..6512
                     /label="lacI"
                     /note="lac repressor"
     CDS             6688..7500
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(7828..7844)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(8455..9600)
                     /label="trfA"
                     /note="trans-acting replication protein that binds to and
                     activates oriV"
     regulatory      complement(9709..9758)
                     /label="neo"
                     /note="neo"
                     /regulatory_class="promoter"
     rep_origin      complement(9992..10522)
                     /direction=LEFT
                     /label="oriV"
                     /note="incP origin of replication"
     CDS             complement(10772..11629)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(11630..11734)
                     /label="AmpR promoter"
     oriT            11911..12019
                     /label="oriT"
                     /note="incP origin of transfer"
     primer_bind     complement(12106..12122)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tctgcatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
       61 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      121 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
      181 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
      241 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
      301 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
      361 ccaagctttc ctgacggaat gttaattctc gttgaccctg agcaggctgt tgagccaggt
      421 gatttctgca tagccagact tgggggtgat gagtttacct tcaagaaact gatcagggat
      481 agcggtcagg tgtttttaca accactaaac ccacagtacc caatgatccc atgcaatgag
      541 agttgttccg ttgtggggaa agttatcgct agtcagtggc ctgaagagac gtttggctga
      601 tcggcaaggt gttctggtcg gcgggggggg gggggggggg gggatcccgg agtcgtattg
      661 atttggcgtt acgcgaacgc gaagtccgac tctaagatgt cacggaggtt caagttacct
      721 ttagccggaa gtgctggcat tttgtccaat tgagactcgt gcaactggtc agcgaactgg
      781 tcgtagaaat cagccagtac atcacaagac tcatatgtgt caaccatagt ttcgcgcact
      841 gctttgaaca ggttcgcagc gtcagccgga atggtaccga aggagtcgtg aatcagtgca
      901 aaagattcga ttccgtactt ctcgtgtgcc cacactacag tcttacgaag gtggctaccg
      961 tcttggctgt gtacaaagtt aggagcgata ccagactcct gtttgtgtgc atcaatctcg
     1021 ctatctttgt tggtgttaat ggtaggctgt aagcggaact gaccgaggaa catcaggttc
     1081 aagcgcgtct gaataggctt cttgtattcc tgccacacag ggaaaccatc aggagttacc
     1141 caatgcacag cgcaacgctt gcgaagaatc tctccagtct tcttatcttt gacctcagca
     1201 gccagcagct tagcagcaga cttaagccag ttcattgctt caaccgcagc taccaccgtc
     1261 acgctcacag attcccaaat cagcttagcc atgtatccag cagcctgatt cggctgagtg
     1321 aacatcagac ccttgccgga atcaatagct ggctgaatgg tatcttccag cacttgttga
     1381 cggaagccga actctttgga cccgtaagcc agcgtcatga ctgaacgctt agtcacactg
     1441 cgagtaacac cgtaagccag ccattgacca gccagtgcct tagtgcccag cttgactttc
     1501 tcagagattt caccagtgtt ctcatcggtc acggtaacta cttcgttatc ggtcccattg
     1561 attgcgtctg cttgtagaat ctcgttgact ttcttagcaa caatcccgta gatgtcctga
     1621 acggtttcac taggaagcaa gttaaccgcg cgaccaccta cctcatctcg gagcatcgcg
     1681 gagaagtgct ggatgccaga gcaagacccg tcaaacgcca gcggaaggga gcagttatag
     1741 ctcaggccgt ggtgctgtac cccagcgtac tcaaagcaga acgcaaggaa gcagaacgga
     1801 gaatcttgct cagcccacca agtgttctcc agtggagact tagcgcaagc catgatgttc
     1861 tcgtggtttt cctcaatgaa cttgatgcgc tcagggaacg gaaccttatc gacacccgca
     1921 cagtttgcac cgtggatttt cagccagtag taaccttcct taccgattgg tttacctttc
     1981 gccagcgtaa gcagtccttt ggtcatatcg ttaccttgcg ggttgaacat tgacacagcg
     2041 taaacacgac cgcgccagtc catgttgtaa gggaaccaga tggccttatg gttagcaaac
     2101 ttattggctt gctcaagcat gaactcaagg ctgatacggc gagacttgcg agccttgtcc
     2161 ttgcggtaca cagcagcggc agcacgtttc cacgcggtga gagcctcagg attcatgtcg
     2221 atgtcttccg gtttcatcgg gagttcttca cgctcaatcg cagggatgtc ctcgaccgga
     2281 caatgcttcc acttggtgat tacgttggcg accgctagga ctttcttgtt gattttccat
     2341 gcggtgtttt gcgcaatgtt aatcgctttg tacacctcag gcatgtaaac gtcttcgtag
     2401 cgcatcagtg ctttcttact gtgagtacgc accagcgcca gaggacgacg accgttagcc
     2461 caatagccac caccagtaat gccagtccac ggcttaggag gaactacgca aggttggaac
     2521 atcggagaga tgccagccag cgcacctgca cgggttgcga tagcctcagc gtattcaggt
     2581 gcgagttcga tagtctcaga gtcttgacct actacgccag cattttggcg gtgtaagcta
     2641 accattccgg ttgactcaat gagcatctcg atgcagcgta ctcctacatg aatagagtct
     2701 tccttatgcc acgaagacca cgcctcgcca ccgagtagac ccttagagag catgtcagcc
     2761 tcgacaactt gcataaatgc tttcttgtag acgtgcccta cgcgcttgtt gagttgttcc
     2821 tcaacgtttt tcttgaagtg cttagcttca aggtcacgga tacgaccgaa gcgagcctcg
     2881 tcctcaatgg cccgaccgat tgcgcttgct acagcctgaa cggttgtatt gtcagcactg
     2941 gttaggcaag ccagagtggt cttaatggtg atgtacgcta cggcttccgg cttgatttct
     3001 tgcaggaact ggaaggctgt cgggcgcttg ccgcgcttag ctttcacttc ctcaaaccag
     3061 tcgttgatgc gtgcaatcat cttagggagt agggtagtga tgagaggctt ggcggcagcg
     3121 ttatccgcaa cctcaccagc tttaagttga cgctcaaaca tcttgcggaa gcgtgcttca
     3181 cccatctcgt aagactcatg ctcaagggcc aactgttcgc gagctaaacg ctcaccgtaa
     3241 tggtcagcca gagtgttgaa cgggatagca gccagttcga tgtcagagaa gtcgttctta
     3301 gcgatgttaa tcgtgttcat ttagtgcctc ttccagttag taaatccggg atccactcgt
     3361 tattctcgga cgagtgttca gtaatgaacc tctggagaga accatgtata tgatcgttat
     3421 ctgggttgga cttctgcttt taagcccaga taactggcct gaatatgtta atgagagaat
     3481 cggtattcct catgtgtggc atgttttcgt ctttgctctt gcattttcgc tagaggtcga
     3541 aattctgtgt gaaattgtta tccgctcaga attttctgta tgaggttttg ctaaacaact
     3601 ttcaacagtt tcagcggagt gagaatagaa aggaacaact aaaggaattg cgaataataa
     3661 ttttttcacg ttgaaaatct ccaaaaaaaa aggctccaaa aggagccttt aattgtatcg
     3721 gtttatcagc ttgctttcga ggtgaatttc gaccctctag aggtcgaaat tctgtgtgaa
     3781 attgttatcc gctcagaatt ttctgtatga ggttttgcta aacaactttc aacagtttca
     3841 gcggagtgag aatagaaagg aacaactaaa ggaattgcga ataataattt tttcacgttg
     3901 aaaatctcca aaaaaaaagg ctccaaaagg agcctttaat tgtatcggtt tatcagcttg
     3961 ctttcgaggt gaatttcgac cctctagcaa ttaatgtgca tcgattatca gctattgcca
     4021 gcgccagata taagcgattt aagctaagaa aacgcattaa gatgcaaaac gataaagtgc
     4081 gatcagtaat tcaaaacctt acagaagagc aatctatggt tttgtgcgca gcccttaatg
     4141 aaggcaggaa gtatgtggtt acatcaaaac aattcccata cattagtgag ttgattgagc
     4201 ttggtgtgtt gaacaaaact ttttcccgat ggaatggaaa gcatatatta ttccctattg
     4261 aggatattta ctggactgaa ttagttgcca gctatgatcc atataatatt gagataaagc
     4321 caaggccaat atctaagtaa ctagataaga ggaatcgatt ttcccttaat tttctggcgt
     4381 ccactgcatg ttatgccgcg ttcgccaggc ttgctgtacc atgtgcgctg attcttgcgc
     4441 tcaatacgtt gcaggttgct ttcaatctgt ttgtggtatt cagccagcac tgtaaggtct
     4501 atcggattta gtgcgctttc tactcgtgat ttcggtttgc gattcagcga gagaataggg
     4561 cggttaactg gttttgcgct taccccaacc aacaggggat ttgctgcttt ccattgagcc
     4621 tgtttctctg cgcgacgttc gcggcggcgt gtttgtgcat ccatctggat tctcctgtca
     4681 gttagctttg gtggtgtgtg gcagttgtag tcctgaacga aaaccccccg cgattggcac
     4741 attggcagct aatccggaat cgcacttacg gccaatgctt cgtttcgtat cacacacccc
     4801 aaagccttct gctttgaatg ctgcccttct tcagggctta atttttaaga gcgtcacctt
     4861 catggtggtc agtgcgtcct gctgatgggg ttataattac ctcaggtcga cgtcccatgt
     4921 gcaggtgctg aattctgtgt gaaattgtta tccgctcaca attgaatcta ttataattgt
     4981 tatccgctca caaagcaaat aaatttttta tgatttctcg accgatgccc ttgagagcct
     5041 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     5101 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     5161 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     5221 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     5281 agcaggccat tatcgccggc atggcggccg gtcgacaatt cgcgcgcgaa ggcgaagcgg
     5341 catgcattta cgttgacacc atcgaatggt gcaaaacctt tcgcggtatg gcatgatagc
     5401 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
     5461 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
     5521 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
     5581 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
     5641 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
     5701 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
     5761 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
     5821 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
     5881 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
     5941 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
     6001 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
     6061 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
     6121 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
     6181 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     6241 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     6301 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     6361 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     6421 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6481 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
     6541 cgcgaattgt cgactctaga ggatcggggg gggggggaaa gccacgttgt gtctcaaaat
     6601 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     6661 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
     6721 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     6781 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
     6841 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     6901 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     6961 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa
     7021 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     7081 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     7141 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     7201 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
     7261 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     7321 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     7381 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     7441 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     7501 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     7561 ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc
     7621 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct
     7681 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc
     7741 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc cccccccccc
     7801 ctgcaggtcg acggatccgg ggaattcact ggccgtcgtt ttacaacgtc gtgactggga
     7861 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7921 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7981 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata
     8041 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
     8101 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     8161 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     8221 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     8281 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     8341 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     8401 caataatcat gcaggtgctg aacccccagc cggaactgac cccacaaggc cctagcgttt
     8461 gcaatgcacc aggtcatcat tgacccaggc gtgttccacc aggccgctgc ctcgcaactc
     8521 ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg cgggtggaat ccgatccgca
     8581 catgaggcgg aaggtttcca gcttgagcgg gtacggctcc cggtgcgagc tgaaatagtc
     8641 gaacatccgt cgggccgtcg gcgacagctt gcggtacttc tcccatatga atttcgtgta
     8701 gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc aggacctggc aacgggacgt
     8761 tttcttgcca cggtccagga cgcggaagcg gtgcagcagc gacaccgatt ccaggtgccc
     8821 aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg cgcgacaggc attcctcggc
     8881 cttcgtgtaa taccggccat tgatcgacca gcccaggtcc tggcaaagct cgtagaacgt
     8941 gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac tccaacacct gctgccacac
     9001 cagttcgtca tcgtcggccc gcagctcgac gccggtgtag gtgatcttca cgtccttgtt
     9061 gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg attttcttgt tgcgcgtggt
     9121 gaacagggca gagcgggccg tgtcgtttgg catcgctcgc atcgtgtccg gccacggcgc
     9181 aatatcgaac aaggaaagct gcatttcctt gatctgctgc ttcgtgtgtt tcagcaacgc
     9241 ggcctgcttg gcctcgctga cctgttttgc caggtcctcg ccggcggttt ttcgcttctt
     9301 ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc gccaaacctg ccgcctcctg
     9361 ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg ggcagggcag ggggagccag
     9421 ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg accatcgagc cgacggactg
     9481 gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg atggtttcgg catcctcggc
     9541 ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc cggtcaaacg tccgattcat
     9601 tcaccctcct tgcgggattg ccccggaatt aattccccgg atcgatccgt cgatcttgat
     9661 cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc
     9721 ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc
     9781 gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt
     9841 gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc
     9901 tgcggactgg ctttctacgt ggctgccatt tttggggtga ggtcgttcgc ggccgagggg
     9961 cgcagcccct ggggggatgg ggtgccgcgt tagcgggccg ggagggttcg agaagggggg
    10021 gcacccccct tcggcgtgcg cggtcacgcg ccagggcgca gccctggtta aaaacaaggt
    10081 ttataaatat tggtttaaaa gcaggttaaa agacaggtta gcggtggccg aaaaacgggc
    10141 ggaaaccctt gcaaatgctg gattttctgc ctgtggacag cccctcaaat gtcaataggt
    10201 gcgcccctca tctgtcatca ctctgcccct caagtgtcaa ggatcgcgcc cctcatctgt
    10261 cagtagtcgc gcccctcaag tgtcaatacc gcagggcact tatccccagg cttgtccaca
    10321 tcatctgtgg gaaactcgcg taaaatcagg cgttttcgcc gatttgcgag gctggccagc
    10381 tccacgtcgc cggccgaaat cgagcctgcc cctcatctgt caacgccgcg ccgggtgagt
    10441 cggcccctca agtgtcaacg tccgcccctc atctgtcagt gagggccaag ttttccgcgt
    10501 ggtatccaca acgccggcgg ccctacatgg ctctgctgta gtgagtgggt tgcgctccgg
    10561 cagcggtcct gatcccccgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    10621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    11101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    11161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    11221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    11281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    11341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
    11401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    11461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    11521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    11581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    11641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11761 ggtcgatcga cggatctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt
    11821 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga
    11881 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc
    11941 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc
    12001 tgctctgcga ggctggccgt aagctctaag aaaccattat tatcatgaca ttaacctata
    12061 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattcactgg ccgtcgtttt
    12121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
    12181 ccctttcgcc agcaga
//
