LOCUS       40924_25921        7682 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pJB785TTKm1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Santos PM, Di Bartolo I, Blatny JM, Zennaro E, Valla S.
  TITLE     New broad-host-range promoter probe vectors based on the plasmid RK2
            replicon
  JOURNAL   FEMS Microbiol. Lett. 195 (1), 91-96 (2001)
  PUBMED    11167001
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Santos PM, Di Bartolo I, Blatny JM, Zennaro E, Valla S.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-NOV-2000) Biology, University of Rome 'Roma Tre', 
            Viale Marconi 446, Rome, RM 00146, Italy
REFERENCE   3  (bases 1 to 7682)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7682)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2001"; volume: "195"; issue: "1"; pages: 
            "91-96"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-NOV-2000) Biology, University of Rome 'Roma Tre', Viale Marconi 
            446, Rome, RM 00146, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(67..175)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        352..456
                     /label=AmpR promoter
     CDS             457..1314
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1564..2094
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      2316..2496
                     /label=Pneo
                     /note="Pneo"
                     /regulatory_class="promoter"
     CDS             2486..3631
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     CDS             4248..4946
                     /codon_start=1
                     /gene="kan"
                     /product="aminoglycoside-3'-phosphotransferase"
                     /label=kan
                     /note="confers kanamycin resistance"
                     /protein_id="AAK13425.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAAC"
     gene            4248..4946
                     /gene="kan"
                     /label=kan
     regulatory      5010..5476
                     /label=bidirectional
                     /note="bidirectional"
                     /regulatory_class="terminator"
     CDS             complement(5591..7240)
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      complement(7258..7285)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(7377..7463)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
       61 agcttacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
      121 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
      181 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
      241 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
      301 agcggaaaag atccgtcgat cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
      361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      661 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
      721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1501 tttctgcggg ggatcaggac cgctgccgga gcgcaaccca ctcactacag cagagccatg
     1561 tagggccgcc ggcgttgtgg ataccacgcg gaaaacttgg ccctcactga cagatgaggg
     1621 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     1681 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     1741 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     1801 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     1861 cagagtgatg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     1921 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     1981 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt
     2041 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
     2101 gcaccccatc cccccagggg ctgcgcccct cggccgcgaa cgacctcacc ccaaaaatgg
     2161 cagccacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     2221 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     2281 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     2341 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     2401 cgccaaggat ctgatggcgc aggggatcaa gatcgacgga tcgatccggg gaattaattc
     2461 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     2521 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     2581 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     2641 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     2701 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     2761 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     2821 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     2881 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     2941 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     3001 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     3061 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     3121 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     3181 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     3241 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     3301 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     3361 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     3421 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     3481 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     3541 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     3601 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     3661 gggttcagca gccacctgca tcgccatcct atggaactgc ctcggtgagt tttctccttc
     3721 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
     3781 gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg
     3841 gcagagcatt acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct
     3901 gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa
     3961 aagttcaaaa tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc
     4021 actttctggc tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca
     4081 cgaggcagac ctcagcgccc ccccccccct gcaggtcgac ctgcaggggg gggggggaaa
     4141 gccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     4201 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc
     4261 aacgggaaac gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg
     4321 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg
     4381 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg
     4441 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca
     4501 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa
     4561 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg
     4621 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc
     4681 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg
     4741 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc
     4801 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta
     4861 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc
     4921 gataccagga tcttgcagct tgctagaggg tcagctttat gcttgtaaac cgttttgtga
     4981 aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc
     5041 tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg
     5101 tgccaagctc tcgggtaaca tcaaggcccg atccttggta gtcggcaaat aatgtctaac
     5161 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     5221 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     5281 gcataataag ccctacacaa attgggagat atatcatgaa aggctggctt tttcttgtta
     5341 tcgcaatagt tggcgaagta atcgcaacat ccgcattaaa atctagcgag ggctttacta
     5401 agctgatccg gtggatgacc ttttgaatga cctttaatag attatattac taattaattg
     5461 gggaccctag aggtcccctt ttttatttta aaaatttttt cacaaaacgg tttacaagca
     5521 taaagctgac tctagctaga ggatcccaat agctaagaat ttcgtcatcg ctgaatacag
     5581 ttacatttta caatttggac tttccgccct tcttggcctt tatgaggatc tctctgattt
     5641 ttcttgcgtc gagttttccg gtaagacctt tcggtacttc gtccacaaac acaactcctc
     5701 cgcgcaactt tttcgcggtt gttacttgac tggccacgta atccacgatc tctttttccg
     5761 tcatcgtctt tccgtgctcc aaaacaacaa cggcggcggg aagttcaccg gcgtcatcgt
     5821 cgggaagacc tgccacgccc gcgtcgaaga tgttggggtg ttgtaacaat atcgattcca
     5881 attcagcggg ggccacctga tatcctttgt atttaattaa agacttcaag cggtcaacta
     5941 tgaagaagtg ttcgtcttcg tcccagtaag ctatgtctcc agaatgtagc catccatcct
     6001 tgtcaatcaa ggcgttggtc gcttccggat tgtttacata accggacata atcataggtc
     6061 ctctgacaca taattcgcct ctctgattaa cgcccagcgt tttcccggta tccagatcca
     6121 caaccttcgc ttcaaaaaat ggaacaactt taccgaccgc gcccggttta tcatccccct
     6181 cgggtgtaat cagaatagct gatgtagtct cagtgagccc atatccttgt cgtatccctg
     6241 gaagatggaa gcgttttgca accgcttccc cgacttcttt cgaaagaggt gcgcccccag
     6301 aagcaatttc gtgtaaatta gataaatcgt atttgtcaat cagagtgctt ttggcgaaga
     6361 atgaaaatag ggttggtact agcaacgcac tttgaatttt gtaatcctga agggatcgta
     6421 aaaacagctc ttcttcaaat ctatacatta agacgactcg aaatccacat atcaaatatc
     6481 cgagtgtagt aaacattcca aaaccgtgat ggaatggaac aacacttaaa atcgcagtat
     6541 ccggaatgat ttgattgcca aaaataggat ctctggcatg cgagaatctg acgcaggcag
     6601 ttctatgcgg aagggccaca cccttaggta acccagtaga tccagaggaa ttcattatca
     6661 gtgcaattgt tttgtcacga tcaaaggact ctggtacaaa atcgtattca ttaaaaccgg
     6721 gaggtagatg agatgtgacg aacgtgtaca tcgactgaaa tccctggtaa tccgttttag
     6781 aatccatgat aataattttc tggattattg gtaatttttt ttgcacgttc aaaatttttt
     6841 gcaacccctt tttggaaaca aacactacgg taggctgcga aatgttcata ctgttgagca
     6901 attcacgttc attataaatg tcgttcgcgg gcgcaactgc aactccgata aataacgcgc
     6961 ccaacaccgg cataaagaat tgaagagagt tttcactgca tacgacgatt ctgtgatttg
     7021 tattcagccc atatcgtttc atagcttctg ccaaccgaac ggacatttcg aagtattccg
     7081 cgtacgtgat gttcacctcg atatgtgcat ctgtaaaagc aattgttcca ggaaccaggg
     7141 cgtatctctt catagcctta tgcagttgct ctccagcggt tccatcctct agaggataga
     7201 atggcgccgg gcctttcttt atgtttttgg cgtcttccat gggcgcagat ctacgcaaaa
     7261 aggccatccg tcaggatggc cttctgctta atttgatgcc tggcagttta tggcgggcgt
     7321 cctgcccgcc accctccggg ccgttgcttc gcaacgttca aatccgctcc cggcggattt
     7381 gtcctactca ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc
     7441 gactgagcct ttcgttttat ttgatgcctg gcagttccct actctcgcat ggggagaccc
     7501 cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac
     7561 caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt
     7621 aatctgtatc aggctgaaaa tcttctctca tccgccagga tctgcgaatt cctcgaccaa
     7681 tt
//
