LOCUS       40924_25916        6431 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Promoter probe vector pJB785TT, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Santos PM, Di Bartolo I, Blatny JM, Zennaro E, Valla S.
  TITLE     New broad-host-range promoter probe vectors based on the plasmid RK2
            replicon
  JOURNAL   FEMS Microbiol. Lett. 195 (1), 91-96 (2001)
  PUBMED    11167001
REFERENCE   2  (bases 1 to 6431)
  AUTHORS   Blatny JM, Santos PM, Di Bartolo I, Valla S, Zennaro E.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-SEP-1999) Department of Biology, Third University of 
            Rome, Viale Marconi 446, Rome 00146, Italy
REFERENCE   3  (bases 1 to 6431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbiol. Lett."; date: "2001"; volume: "195"; issue: "1"; pages: 
            "91-96"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-SEP-1999) Department of Biology, Third University of Rome, Viale
            Marconi 446, Rome 00146, Italy"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            complement(67..175)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        352..456
                     /label=AmpR promoter
     CDS             457..1314
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1564..2094
                     /label=oriV
                     /note="incP origin of replication"
     regulatory      2316..2496
                     /label=Pneo
                     /note="Pneo"
                     /regulatory_class="promoter"
     CDS             2486..3631
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     regulatory      3702..4276
                     /label=bidirectional
                     /note="bidirectional"
                     /regulatory_class="terminator"
     CDS             complement(4340..5989)
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      complement(6007..6034)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(6126..6212)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
ORIGIN
        1 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
       61 agcttacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
      121 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
      181 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
      241 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
      301 agcggaaaag atccgtcgat cgacccaggt ggcacttttc ggggaaatgt gcgcggaacc
      361 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
      421 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
      481 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
      541 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      601 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      661 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa
      721 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      781 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      841 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      901 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      961 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     1021 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     1081 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     1141 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     1201 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     1261 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     1321 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     1381 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     1441 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     1501 tttctgcggg ggatcaggac cgctgccgga gcgcaaccca ctcactacag cagagccatg
     1561 tagggccgcc ggcgttgtgg ataccacgcg gaaaacttgg ccctcactga cagatgaggg
     1621 gcggacgttg acacttgagg ggccgactca cccggcgcgg cgttgacaga tgaggggcag
     1681 gctcgatttc ggccggcgac gtggagctgg ccagcctcgc aaatcggcga aaacgcctga
     1741 ttttacgcga gtttcccaca gatgatgtgg acaagcctgg ggataagtgc cctgcggtat
     1801 tgacacttga ggggcgcgac tactgacaga tgaggggcgc gatccttgac acttgagggg
     1861 cagagtgatg acagatgagg ggcgcaccta ttgacatttg aggggctgtc cacaggcaga
     1921 aaatccagca tttgcaaggg tttccgcccg tttttcggcc accgctaacc tgtcttttaa
     1981 cctgctttta aaccaatatt tataaacctt gtttttaacc agggctgcgc cctggcgcgt
     2041 gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg
     2101 gcaccccatc cccccagggg ctgcgcccct cggccgcgaa cgacctcacc ccaaaaatgg
     2161 cagccacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     2221 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     2281 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     2341 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
     2401 cgccaaggat ctgatggcgc aggggatcaa gatcgacgga tcgatccggg gaattaattc
     2461 cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt gaccggaagg catacaggca
     2521 agaactgatc gacgcggggt tttccgccga ggatgccgaa accatcgcaa gccgcaccgt
     2581 catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg atggtccagc aagctacggc
     2641 caagatcgag cgcgacagcg tgcaactggc tccccctgcc ctgcccgcgc catcggccgc
     2701 cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt ttggcgaagt cgatgaccat
     2761 cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc gccggcgagg acctggcaaa
     2821 acaggtcagc gaggccaagc aggccgcgtt gctgaaacac acgaagcagc agatcaagga
     2881 aatgcagctt tccttgttcg atattgcgcc gtggccggac acgatgcgag cgatgccaaa
     2941 cgacacggcc cgctctgccc tgttcaccac gcgcaacaag aaaatcccgc gcgaggcgct
     3001 gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag atcacctaca ccggcgtcga
     3061 gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg ttggagtacg cgaagcgcac
     3121 ccctatcggc gagccgatca ccttcacgtt ctacgagctt tgccaggacc tgggctggtc
     3181 gatcaatggc cggtattaca cgaaggccga ggaatgcctg tcgcgcctac aggcgacggc
     3241 gatgggcttc acgtccgacc gcgttgggca cctggaatcg gtgtcgctgc tgcaccgctt
     3301 ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag gtcctgatcg acgaggaaat
     3361 cgtcgtgctg tttgctggcg accactacac gaaattcata tgggagaagt accgcaagct
     3421 gtcgccgacg gcccgacgga tgttcgacta tttcagctcg caccgggagc cgtacccgct
     3481 caagctggaa accttccgcc tcatgtgcgg atcggattcc acccgcgtga agaagtggcg
     3541 cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc ggcctggtgg aacacgcctg
     3601 ggtcaatgat gacctggtgc attgcaaacg ctagggcctt gtggggtcag ttccggctgg
     3661 gggttcagca gccacctgca tcgcaagctt gctagagggt cagctttatg cttgtaaacc
     3721 gttttgtgaa aaaattttta aaataaaaaa ggggacctct agggtcccca attaattagt
     3781 aatataatct attaaaggtc attcaaaagg tcatccaccg gatcaattcc cctgctcgcg
     3841 caggctgggt gccaagctct cgggtaacat caaggcccga tccttggtag tcggcaaata
     3901 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     3961 atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt
     4021 tttaagcgtg cataataagc cctacacaaa ttgggagata tatcatgaaa ggctggcttt
     4081 ttcttgttat cgcaatagtt ggcgaagtaa tcgcaacatc cgcattaaaa tctagcgagg
     4141 gctttactaa gctgatccgg tggatgacct tttgaatgac ctttaataga ttatattact
     4201 aattaattgg ggaccctaga ggtccccttt tttattttaa aaattttttc acaaaacggt
     4261 ttacaagcat aaagctgact ctagctagag gatcccaata gctaagaatt tcgtcatcgc
     4321 tgaatacagt tacattttac aatttggact ttccgccctt cttggccttt atgaggatct
     4381 ctctgatttt tcttgcgtcg agttttccgg taagaccttt cggtacttcg tccacaaaca
     4441 caactcctcc gcgcaacttt ttcgcggttg ttacttgact ggccacgtaa tccacgatct
     4501 ctttttccgt catcgtcttt ccgtgctcca aaacaacaac ggcggcggga agttcaccgg
     4561 cgtcatcgtc gggaagacct gccacgcccg cgtcgaagat gttggggtgt tgtaacaata
     4621 tcgattccaa ttcagcgggg gccacctgat atcctttgta tttaattaaa gacttcaagc
     4681 ggtcaactat gaagaagtgt tcgtcttcgt cccagtaagc tatgtctcca gaatgtagcc
     4741 atccatcctt gtcaatcaag gcgttggtcg cttccggatt gtttacataa ccggacataa
     4801 tcataggtcc tctgacacat aattcgcctc tctgattaac gcccagcgtt ttcccggtat
     4861 ccagatccac aaccttcgct tcaaaaaatg gaacaacttt accgaccgcg cccggtttat
     4921 catccccctc gggtgtaatc agaatagctg atgtagtctc agtgagccca tatccttgtc
     4981 gtatccctgg aagatggaag cgttttgcaa ccgcttcccc gacttctttc gaaagaggtg
     5041 cgcccccaga agcaatttcg tgtaaattag ataaatcgta tttgtcaatc agagtgcttt
     5101 tggcgaagaa tgaaaatagg gttggtacta gcaacgcact ttgaattttg taatcctgaa
     5161 gggatcgtaa aaacagctct tcttcaaatc tatacattaa gacgactcga aatccacata
     5221 tcaaatatcc gagtgtagta aacattccaa aaccgtgatg gaatggaaca acacttaaaa
     5281 tcgcagtatc cggaatgatt tgattgccaa aaataggatc tctggcatgc gagaatctga
     5341 cgcaggcagt tctatgcgga agggccacac ccttaggtaa cccagtagat ccagaggaat
     5401 tcattatcag tgcaattgtt ttgtcacgat caaaggactc tggtacaaaa tcgtattcat
     5461 taaaaccggg aggtagatga gatgtgacga acgtgtacat cgactgaaat ccctggtaat
     5521 ccgttttaga atccatgata ataattttct ggattattgg taattttttt tgcacgttca
     5581 aaattttttg caaccccttt ttggaaacaa acactacggt aggctgcgaa atgttcatac
     5641 tgttgagcaa ttcacgttca ttataaatgt cgttcgcggg cgcaactgca actccgataa
     5701 ataacgcgcc caacaccggc ataaagaatt gaagagagtt ttcactgcat acgacgattc
     5761 tgtgatttgt attcagccca tatcgtttca tagcttctgc caaccgaacg gacatttcga
     5821 agtattccgc gtacgtgatg ttcacctcga tatgtgcatc tgtaaaagca attgttccag
     5881 gaaccagggc gtatctcttc atagccttat gcagttgctc tccagcggtt ccatcctcta
     5941 gaggatagaa tggcgccggg cctttcttta tgtttttggc gtcttccatg ggcgcagatc
     6001 tacgcaaaaa ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagtttat
     6061 ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttcg caacgttcaa atccgctccc
     6121 ggcggatttg tcctactcag gagagcgttc accgacaaac aacagataaa acgaaaggcc
     6181 cagtctttcg actgagcctt tcgttttatt tgatgcctgg cagttcccta ctctcgcatg
     6241 gggagacccc acactaccat cggcgctacg gcgtttcact tctgagttcg gcatggggtc
     6301 aggtgggacc accgcgctac tgccgccagg caaattctgt tttatcagac cgcttctgcg
     6361 ttctgattta atctgtatca ggctgaaaat cttctctcat ccgccaggat ctgcgaattc
     6421 ctcgaccaat t
//
