LOCUS       62056_13080        6762 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             262..1224
                     /codon_start=1
                     /label=XylS
                     /note="XylS regulator encoded by the Pseudomonas putida TOL
                     plasmid pWWO"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGPPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     primer_bind     1664..1680
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     oriT            complement(1767..1875)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     promoter        2052..2156
                     /label=AmpR promoter
     CDS             2157..3014
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3264..3794
                     /label=oriV
                     /note="incP origin of replication"
     CDS             4186..5331
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFTWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     promoter        complement(6048..6128)
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
ORIGIN
        1 cagatcccct ttatccgcca attcgtcgcc gcatgccccg acagcacgaa cttctggtgt
       61 tctcgcttct taaaaagaac gtcttcgttc tgcttggcgt tatttttgct tggaaaagtg
      121 gtcactgatt gcaaaaagga tggcgcaacg tggcaatggg ggtaacccgt atacgcatca
      181 cgtcgagatg cattttcatc gacttggcgc ctttctacat cacaccaagc agcccacatt
      241 aaaataagag aaccgtgaac tatggatttt tgcttattga acgagaaaag tcagatcttc
      301 gtccacgccg agccctatgc agtctccgat tatgttaacc agtatgtcgg tacgcactct
      361 attcgcctgc ccaagggcgg gcccccggca ggcaggctgc accacagaat cttcggatgc
      421 ctcgacctgt gtcgaatcag ctacggcggt agcgtgaggg taatctcgcc tggattagag
      481 acctgttatc atctgcaaat aatactcaaa ggccattgcc tgtggcgtgg ccatggccag
      541 gagcactatt ttgcgccggg cgaactattg ctgctcaatc cggatgacca agccgacctg
      601 acctattcag aagattgcga gaaatttatc gttaaattgc cctcagtggt ccttgatcgg
      661 gcatgcagtg acaacaattg gcacaagccg agggagggta tccgtttcgc cgcgcgacac
      721 aatctccagc aactcgatgg ctttatcaat ctactcgggt tagtttgtga cgaagcggaa
      781 catacaaagt cgatgcctcg ggtccaagag cactatgcgg ggatcatcgc ttccaagctg
      841 ctcgaaatgc tgggcagcaa tgtcagccgt gaaattttca gcaaaggtaa cccgtctttc
      901 gagcgagtcg ttcaattcat tgaggagaat ctcaaacgga atatcagcct tgagcggtta
      961 gcggagctgg cgatgatgag tccacgctcg ctctacaatt tgttcgagaa gcatgccggc
     1021 accacgccga agaactacat ccgcaaccgc aagctcgaaa gcatccgcgc ctgcttgaac
     1081 gatcccagtg ccaatgtgcg tagtataact gagatagccc tagactacgg cttcttacat
     1141 ttgggacgct tcgctgaaaa ctataggagc gcgttcggcg agttgccttc cgacaccctg
     1201 cgtcaatgca aaaaggaagt ggcttgatta cgaacgtagc cgaagaaggg atgggttggc
     1261 atcgcccggc tttcttagac actctccaag ctctgaaata gcgttttaca aactccactg
     1321 gctatagtgc cggcgtttgc ccgcgctctc cgtggccgcg tcccaatgca ggtcgggttc
     1381 ctcgacccga agtgcccttg ggcatctcca cttcagcgtc tgaccttgct ggccagttcg
     1441 ccgccgatgc cgaagtggcc cttggccatc tccgtgataa tcactcgcac gctggtcagc
     1501 ggcgcatcca gggagcgcga gatggcctcg ctgacttccc gaatgagggt ttccttctgc
     1561 tcgtcgctgc ggccttcaag gatgtggatc tgctggccga aagggggatg tgctgcaagg
     1621 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     1681 gaattaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     1741 tgataataat ggtttcttag agcttacggc cagcctcgca gagcaggatt cccgttgagc
     1801 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact
     1861 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     1921 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     1981 accccgaagc agggttatgc agcggaaaag atccgtcgat cgacccaggt ggcacttttc
     2041 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2101 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2161 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2221 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2281 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     2341 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     2401 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2461 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2521 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2581 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2641 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2701 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     2761 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     2821 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2881 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2941 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3001 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3061 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3121 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3181 gatcttcttg agatcctttt tttctgcggg ggatcaggac cgctgccgga gcgcaaccca
     3241 ctcactacag cagagccatg tagggccgcc ggcgttgtgg ataccacgcg gaaaacttgg
     3301 ccctcactga cagatgaggg gcggacgttg acacttgagg ggccgactca cccggcgcgg
     3361 cgttgacaga tgaggggcag gctcgatttc ggccggcgac gtggagctgg ccagcctcgc
     3421 aaatcggcga aaacgcctga ttttacgcga gtttcccaca gatgatgtgg acaagcctgg
     3481 ggataagtgc cctgcggtat tgacacttga ggggcgcgac tactgacaga tgaggggcgc
     3541 gatccttgac acttgagggg cagagtgatg acagatgagg ggcgcaccta ttgacatttg
     3601 aggggctgtc cacaggcaga aaatccagca tttgcaaggg tttccgcccg tttttcggcc
     3661 accgctaacc tgtcttttaa cctgctttta aaccaatatt tataaacctt gtttttaacc
     3721 agggctgcgc cctggcgcgt gaccgcgcac gccgaagggg ggtgcccccc cttctcgaac
     3781 cctcccggcc cgctaacgcg gcaccccatc cccccagggg ctgcgcccct cggccgcgaa
     3841 cgacctcacc ccaaaaatgg cagccacgta gaaagccagt ccgcagaaac ggtgctgacc
     3901 ccggatgaat gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagagaaa
     3961 gcaggtagct tgcagtgggc ttacatggcg atagctagac tgggcggttt tatggacagc
     4021 aagcgaaccg gaattgccag ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt
     4081 aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa gatcgacgga
     4141 tcgatccggg gaattaattc cggggcaatc ccgcaaggag ggtgaatgaa tcggacgttt
     4201 gaccggaagg catacaggca agaactgatc gacgcggggt tttccgccga ggatgccgaa
     4261 accatcgcaa gccgcaccgt catgcgtgcg ccccgcgaaa ccttccagtc cgtcggctcg
     4321 atggtccagc aagctacggc caagatcgag cgcgacagcg tgcaactggc tccccctgcc
     4381 ctgcccgcgc catcggccgc cgtggagcgt tcgcgtcgtc tcgaacagga ggcggcaggt
     4441 ttggcgaagt cgatgaccat cgacacgcga ggaactatga cgaccaagaa gcgaaaaacc
     4501 gccggcgagg acctggcaaa acaggtcagc gaggccaagc aggccgcgtt gctgaaacac
     4561 acgaagcagc agatcaagga aatgcagctt tccttgttcg atattgcgcc gtggccggac
     4621 acgatgcgag cgatgccaaa cgacacggcc cgctctgccc tgttcaccac gcgcaacaag
     4681 aaaatcccgc gcgaggcgct gcaaaacaag gtcattttcc acgtcaacaa ggacgtgaag
     4741 atcacctaca ccggcgtcga gctgcgggcc gacgatgacg aactggtgtg gcagcaggtg
     4801 ttggagtacg cgaagcgcac ccctatcggc gagccgatca ccttcacgtt ctacgagctt
     4861 tgccaggacc tgggctggtc gatcaatggc cggtattaca cgaaggccga ggaatgcctg
     4921 tcgcgcctac aggcgacggc gatgggcttc acgtccgacc gcgttgggca cctggaatcg
     4981 gtgtcgctgc tgcaccgctt ccgcgtcctg gaccgtggca agaaaacgtc ccgttgccag
     5041 gtcctgatcg acgaggaaat cgtcgtgctg tttgctggcg accactacac gaaattcaca
     5101 tgggagaagt accgcaagct gtcgccgacg gcccgacgga tgttcgacta tttcagctcg
     5161 caccgggagc cgtacccgct caagctggaa accttccgcc tcatgtgcgg atcggattcc
     5221 acccgcgtga agaagtggcg cgagcaggtc ggcgaagcct gcgaagagtt gcgaggcagc
     5281 ggcctggtgg aacacgcctg ggtcaatgat gacctggtgc attgcaaacg ctagggcctt
     5341 gtggggtcag ttccggctgg gggttcagca gccaccggca tgcaagcttg ctagagggtc
     5401 agctttatgc ttgtaaaccg ttttgtgaaa aaatttttaa aataaaaaag gggacctcta
     5461 gggtccccaa ttaattagta atataatcta ttaaaggtca ttcaaaaggt catccaccgg
     5521 atcaattccc ctgctcgcgc aggctgggtg ccaagctctc gggtaacatc aaggcccgat
     5581 ccttggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc
     5641 ggcttaactc aagcgttaga tgcactaagc acataattgc tcacagccaa actatcaggt
     5701 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat
     5761 atcatgaaag gctggctttt tcttgttatc gcaatagttg gcgaagtaat cgcaacatcc
     5821 gcattaaaat ctagcgaggg ctttactaag ctgatccggt ggatgacctt ttgaatgacc
     5881 tttaatagat tatattacta attaattggg gaccctagag gtcccctttt ttattttaaa
     5941 aattttttca caaaacggtt tacaagcata aagctgactc tagctagagg atccccgggt
     6001 accgagctcg aattcatatg ttcatgactc cattattatt gtttctgttg cataaagcct
     6061 aaggggtagg cctttctaga gatagccatt ttttgcactc ctgtatccgc ttcttgcaag
     6121 gctggactta tccctatcaa accggacacg tttatgctgg ttatgctggc tgcatggggc
     6181 attagttggg gagccagatt tgtcatggag caggccgttc tgctttatgg atcaggaaaa
     6241 aactatttgt tcttcagtca tggtactgtt ctgatgtacc tgctgtgtgt tttcctggta
     6301 taccgccgtt ggatagctcc gctaccggtc gttggtcaga tcaagctaat tcccatgcat
     6361 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     6421 actcacccag ggattggctg agacgaaaaa catattctca ataaaccctt tagggaaata
     6481 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     6541 atcgtcgtgg tattcactcc agagcgatga aaacgtttca gtttgctcat ggaaaacggt
     6601 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgtaa
     6661 ttacggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     6721 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc ag
//
