LOCUS       V005372                 7067 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005372
VERSION     V005372
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Adeniran A, Stainbrook SC, Bostick JW, Tyo KEJ.
  TITLE     Detection of Peptide Biomarkers by Engineered Yeast Receptors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Pertusi D, Yu J, Adeniran A, Tyo KEJ.
  TITLE     CellSort: A support vector machine algorithm for optimizing and
            simulating FACS
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7067)
  AUTHORS   Tyo KEJ., Adeniran A, Stainbrook SC, Bostick JW.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2016) Chemical and Biological Engineering,
            Northwestern University, 2145 Sheridan Rd, Evanston, IL 60208, USA
REFERENCE   4  (bases 1 to 7067)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7067)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (07-MAR-2016) Chemical and Biological Engineering, Northwestern
            University, 2145 Sheridan Rd, Evanston, IL 60208, USA"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..36)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      39..286
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        complement(305..323)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(333..349)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      494..949
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1083..1883)
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(1884..2104)
                     /label="URA3 promoter"
     misc_feature    complement(2369..2872)
                     /label="CEN/ARS"
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        2909..3013
                     /label="AmpR promoter"
     CDS             3014..3871
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4045..4633
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    4921..4942
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4957..4987
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4995..5011
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5019..5035
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        5056..5074
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5099..5742
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     5748..5764
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    5766..5771
                     /label="Kozak"
                     /note="Kozak"
     CDS             5772..7064
                     /gene="STE2"
                     /label="Pheromone alpha factor receptor"
                     /note="Pheromone alpha factor receptor from Saccharomyces
                     cerevisiae. Accession#: P0CI39"
ORIGIN
        1 aattcgatat caagcttatc gataccgtcg acctcgagtc atgtaattag ttatgtcacg
       61 cttacattca cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc
      121 tgaagtctag gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat
      181 atttcaaatt tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa
      241 aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcggcc ggtacccaat
      301 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
      361 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      421 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      481 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      541 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      601 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      661 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
      721 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
      781 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
      841 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
      901 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ctgatgcggt
      961 attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat
     1021 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt
     1081 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca
     1141 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca
     1201 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg
     1261 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc
     1321 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca
     1381 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc
     1441 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata
     1501 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca
     1561 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag
     1621 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa
     1681 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca
     1741 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc
     1801 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt
     1861 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc
     1921 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat
     1981 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca
     2041 aaaaaatttc aaagaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat
     2101 tgaaaagctg tggtatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     2161 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     2221 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     2281 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     2341 gtcatgataa taatggtttc ttaggacgga tcgcttgcct gtaacttaca cgcgcctcgt
     2401 atcttttaat gatggaataa tttgggaatt tactctgtgt ttatttattt ttatgttttg
     2461 tatttggatt ttagaaagta aataaagaag gtagaagagt tacggaatga agaaaaaaaa
     2521 ataaacaaag gtttaaaaaa tttcaacaaa aagcgtactt tacatatata tttattagac
     2581 aagaaaagca gattaaatag atatacattc gattaacgat aagtaaaatg taaaatcaca
     2641 ggattttcgt gtgtggtctt ctacacagac aagatgaaac aattcggcat taatacctga
     2701 gagcaggaag agcaagataa aaggtagtat ttgttggcga tccccctaga gtcttttaca
     2761 tcttcggaaa acaaaaacta ttttttcttt aatttctttt tttactttct atttttaatt
     2821 tatatattta tattaaaaaa tttaaattat aattattttt atagcacgtg atgaaaagga
     2881 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2941 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3001 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3061 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3121 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3181 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3241 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3301 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3361 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3421 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3481 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3541 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3601 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3661 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3721 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3781 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3841 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3901 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3961 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4021 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4081 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4141 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4201 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4261 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4321 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4381 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4441 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4501 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4561 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4621 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4681 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4741 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4801 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4861 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4921 taatgtgagt tacctcactc attaggcacc ccaggcttta cactttatgc ttccggctcc
     4981 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5041 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tcagtttatc
     5101 attatcaata ctcgccattt caaagaatac gtaaataatt aatagtagtg attttcctaa
     5161 ctttatttag tcaaaaaatt agccttttaa ttctgctgta acccgtacat gcccaaaata
     5221 gggggcgggt tacacagaat atataacatc gtaggtgtct gggtgaacag tttattcctg
     5281 gcatccacta aatataatgg agcccgcttt ttaagctggc atccagaaaa aaaaagaatc
     5341 ccagcaccaa aatattgttt tcttcaccaa ccatcagttc ataggtccat tctcttagcg
     5401 caactacaga gaacaggggc acaaacaggc aaaaaacggg cacaacctca atggagtgat
     5461 gcaacctgcc tggagtaaat gatgacacaa ggcaattgac ccacgcatgt atctatctca
     5521 ttttcttaca ccttctatta ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag
     5581 gttgaaacca gttccctgaa attattcccc tacttgacta ataagtatat aaagacggta
     5641 ggtattgatt gtaattctgt aaatctattt cttaaacttc ttaaattcta cttttatagt
     5701 tagtcttttt tttagtttta aaacaccaga acttagtttc gacggattct agaactagtg
     5761 gatccaaaaa aatgtctgat gcggctcctt cattgagcaa tctattttat gatccaacgt
     5821 ataatcctgg tcaaagcacc attaactaca cttccatata tgggaatgga tctaccatca
     5881 ctttcgatga gttgcaaggt ttagttaaca gtactgttac tcaggccatt atgtttggtg
     5941 tcagatgtgg tgcagctgct ttgactttga ttgtcatgtg gatgacatcg agaagcagaa
     6001 aaacgccgat tttcattatc aaccaagttt cattgttttt aatcattttg cattctgcac
     6061 tctattttaa atatttactg tctaattact cttcagtgac ttacgctctc accggatttc
     6121 ctcagttcat cagtagaggt gacgttcatg tttatggtgc tacaaatata attcaagtcc
     6181 ttcttgtggc ttctattgag acttcactgg tgtttcagat aaaagttatt ttcacaggcg
     6241 acaacttcaa aaggataggt ttgatgctga cgtcgatatc tttcacttta gggattgcta
     6301 cagttaccat gtattttgta agcgctgtta aaggtatgat tgtgacttat aatgatgtta
     6361 gtgccaccca agataaatac ttcaatgcat ccacaatttt acttgcatcc tcaataaact
     6421 ttatgtcatt tgtcctggta gttaaattga ttttagctat tagatcaaga agattccttg
     6481 gtctcaagca gttcgatagt ttccatattt tactcataat gtcatgtcaa tctttgttgg
     6541 ttccatcgat aatattcatc ctcgcataca gtttgaaacc aaaccaggga acagatgtct
     6601 tgactactgt tgcaacatta cttgctgtat tgtctttacc attatcatca atgtgggcca
     6661 cggctgctaa taatgcatcc aaaacaaaca caattacttc agactttaca acatccacag
     6721 ataggtttta tccaggcacg ctgtctagct ttcaaactga tagtatcaac aacgatgcta
     6781 aaagcagtct cagaagtaga ttatatgacc tatatcctag aaggaaggaa acaacatcgg
     6841 ataaacattc ggaaagaact tttgtttctg agactgcaga tgatatagag aaaaatcagt
     6901 tttatcagtt gcccacacct acgagttcaa aaaatactag gataggaccg tttgctgatg
     6961 caagttacaa agagggagaa gttgaacccg tcgacatgta cactcccgat acggcagctg
     7021 atgaggaagc cagaaagttc tggactgaag ataataataa tttatga
//
