LOCUS       40924_25876        6766 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAP8, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Tamura GS, Nittayajarn A, Schoentag DL.
  TITLE     A glutamine transport gene, glnQ, is required for fibronectin 
            adherence and virulence of group B streptococci
  JOURNAL   Infect. Immun. 70 (6), 2877-2885 (2002)
  PUBMED    12010975
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Tamura GS, Schoentag DL, Nittayajarn A.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2001) Pediatrics, Children's Hospital and Regional
            Medical Center and the University of Washington, 4800 Sand Point Way
            NE, Seattle, WA 98105-0371, USA
REFERENCE   3  (bases 1 to 6766)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect. 
            Immun."; date: "2002"; volume: "70"; issue: "6"; pages: "2877-2885"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2001) Pediatrics, Children's Hospital and Regional Medical 
            Center and the University of Washington, 4800 Sand Point Way NE, 
            Seattle, WA 98105-0371, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(32..50)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(69..85)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(93..109)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(117..147)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(162..183)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(471..1059)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1233..2090)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2091..2195)
                     /label=AmpR promoter
     rep_origin      2222..2677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     2819..2835
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2869..4470
                     /label=3' portion of glnP
                     /note="3' portion of glnP"
     CDS             4470..5210
                     /codon_start=1
                     /gene="glnQ"
                     /product="ATP binding protein"
                     /label=glnQ
                     /note="component of glutamine transport system"
                     /protein_id="AAK62567.1"
                     /translation="MAELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGKS
                     TFLRTLNLLESITSGKVVVDGFELSNPKTDIDKARENIGMVFQHFNLFPHMSVLENITF
                     APIELGKESKEAAEKHGMELLEKVGLADKANAKPDSLSGGQKQRVAIARSLAMNPDILL
                     FDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGRFLEDG
                     TPEQIFDTPQHPRLQDFLNKVLNV"
     gene            4470..5210
                     /gene="glnQ"
                     /label=glnQ
     CDS             complement(4952..4969)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     CDS             5360..5710
                     /codon_start=1
                     /gene="ORFX"
                     /product="unknown protein"
                     /label=ORFX
                     /note="near glutamine transport operon"
                     /inference="non-experimental evidence, no additional
                     details recorded"
                     /protein_id="AAK62566.1"
                     /translation="MKDYINRILHFIKEHMTYHVNFIDDFLDIKWEKVSNIHLRFWTTI
                     IAYLVIFILSISTVILNLVLLFQGFLTQNPIIYLLFFITLVCAFYFAYKFITYTPTIVK
                     NALQYIKKLKNV"
     gene            5360..5710
                     /gene="ORFX"
                     /label=ORFX
ORIGIN
        1 ctagagtcga cctgcaggca tgcaagcttt ccctatagtg agtcgtatta gagcttggcg
       61 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
      121 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
      181 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
      241 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      301 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      361 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      421 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      481 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      541 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      601 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      661 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      721 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      781 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      841 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      901 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      961 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1021 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1081 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1141 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1201 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1261 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1321 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1381 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1441 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1501 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1561 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1621 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1681 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1741 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1801 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1861 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1921 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1981 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2041 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2101 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2161 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2221 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2521 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2581 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2641 aacgcgaatt ttaacaaaat attaacgttt acaatttccc attcgccatt caggctgcgc
     2701 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
     2761 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
     2821 aaaacgacgg ccagtgaatt cgagctcggt acccggggat ccgattaagc ttacgccatt
     2881 aacatggaac gcgaagcagt tggtagcttt gcatttgctg tcaaaaaagg tagtggacac
     2941 gataatctaa ttaaagaatt taacacagct tttgcacaaa tgaaatcaga tggcacttat
     3001 aatgacatca tggataaatg gcttggaaaa gacgctacaa aaacaagcgg caaagcaaca
     3061 ggtaatgcca atgaaaaagc aactcctgta aagccaagtt ataaaattgt ttctgattct
     3121 tcattcgcac cattcgaata tcaaaacggt aaagggaaat atactggttt tgatatggaa
     3181 ttaatcacga aaattgctaa acagcaaggt tttaaacttg atatctcaaa tccaggtttt
     3241 gatgccgctt taaatgctgt ccaatctggg caagctgacg gtgttattgc aggagccaca
     3301 atcacagaag cacgccaaaa aatctttgat ttttctgatc cttattacac atctagcgtt
     3361 atcttagcgg ttaaaaaagg aagcaatgtc aaatcatacc aagatttaaa aggaaaaaca
     3421 gttggtgcta aaaatggtac tgcctcatat acttggttat cagaccacgc agataagtac
     3481 aactatcatg ttaaagcatt tgatgaagca tctacaatgt atgatagtat gaactcaggt
     3541 tcaattgatg ctctaatgga tgacgaagcc gttcttgctt acgctattaa tcaaggtcgt
     3601 aaatttgaaa cacctatcaa aggtgaaaaa tcaggcgata tcggatttgc agtgaaaaaa
     3661 ggggcaaatc cagaattaat taaaatgttt aacaacggtc ttgcttcact caaaaaatcg
     3721 ggtgagtacg ataaacttgt taaaaaatac ctttccacag ccagcacttc ttcaaacgat
     3781 aaagctgcta aacctgtaga tgaatcaact attttagggt taatttctaa taactacaaa
     3841 caattgctat ctggtattgg aactacttta agtttaactc ttatctcgtt tgcgattgct
     3901 atggttattg gtattatctt tggtatgatg agcgtatcac caagtaatac tctccgcaca
     3961 atttcaatga tttttgttga tattgtccgt ggtattccac tcatgattgt ggccgctttt
     4021 attttctggg gtattcctaa tttaatcgaa agcatcacag gtcaccaaag tccaattaat
     4081 gacttcgttg ctgctactat cgctctttct ttaaatggtg gtgcgtacat tgctgaaatt
     4141 gtacgtggtg gtattgaagc tgttccttct ggtcaaatgg aagcaagtcg cagcttaggt
     4201 atttcttacg gcaaaactat gcaaaaggtt atcttacctc aagcagtacg ccttatgtta
     4261 ccaaacttta tcaaccaatt tgtcatctca ttaaaggata caacaattgt atcagcaatc
     4321 ggacttgtgg aactcttcca aactggtaaa atcataattg cacgtaacta tcaatcattc
     4381 cgtatgtatg ctatccttgc aattatgtat cttgtaatca ttacccttct cacccgtcta
     4441 gcaaaacgtt tagaaaagag gcttaaataa tggcagaatt aaaaattgat gtccaagatt
     4501 tacacaagtc gtatgggcaa aatgaagttt taaaaggtat tgatgctaaa ttttatgaag
     4561 gtgatgttgt ttgtattatt ggaccttctg gttcaggaaa atcaactttc cttcgcacat
     4621 tgaacttact tgaatcaatc acaagtggaa aagttgttgt ggacggcttt gagttatcaa
     4681 atccaaaaac agacatagac aaagcacgtg aaaatatcgg aatggtattt caacacttca
     4741 atctttttcc acatatgtca gttcttgaaa acatcacttt tgctcctata gaacttggta
     4801 aagaatctaa agaagcagct gaaaagcatg ggatggaatt acttgaaaaa gttggtttag
     4861 ccgataaagc taatgccaaa ccagatagtt tatctggagg acaaaaacaa cgtgtagcta
     4921 tcgcaagaag cttagcgatg aatccagata tcttactttt tgacgaacca acttctgcac
     4981 ttgatccaga aatggttgga gatgttctca atgttatgaa agatttagct gaacaaggta
     5041 tgactatgtt aattgtgaca cacgaaatgg gatttgctcg tcaagttgcc aatcgtgtta
     5101 tctttacaga tggtggtcgt ttcctcgaag atgggacacc tgagcaaatc tttgatacac
     5161 ctcaacaccc acgtctccaa gacttcctta ataaagtttt aaacgtttaa taaaaaaaga
     5221 tgacttcgtg tcatcttttt ttatatcctg atcacaaaca aattataata ggataagttt
     5281 tcttctaaaa ttagcaaaac atcagctaga atgtttgaat aatgttgcac aagggctttc
     5341 agtatgataa tatagcagta tgaaagacta tattaacaga atattgcact ttattaaaga
     5401 acatatgact tatcatgtca actttataga tgacttttta gatattaaat gggaaaaggt
     5461 gagtaacatc catttaaggt tttggacaac tattattgct tatctagtta tttttatctt
     5521 aagtatctct accgtaattc ttaaccttgt acttcttttt caaggattct taacgcaaaa
     5581 ccctattatt tacttactat tttttataac tttagtttgt gctttttatt ttgcttataa
     5641 atttattact tatactccaa ccattgtaaa aaatgctctt cagtatatca aaaagctcaa
     5701 aaacgtttaa aataataaag taatctgtac cttaacactc tatcaattat gttttagagt
     5761 cttttttcat accccaaaat aactaaaaag gatagttact taactatcct ttttcttttg
     5821 ctaatttttc gcgttctaaa cgaagtttac gattaggatt taatgtgtta aagagttctt
     5881 ctaatttttc agcattccag acatcggccg ctgaaacaaa gttcccatct ttatcacgaa
     5941 atgatatcgg tttatctccc ataaaaaacc tccaatcaag tactgcatta atctacaaat
     6001 tcaaattcga agttacctat acgaacaata tctccatcct tagctccgcg ctctcgaagt
     6061 gcttcatcaa cgcccatacc acgtaactga cgagcaaatt tcattattga ttcatcgcgt
     6121 tccatatttg tcataacaaa gagtttttct agcttatcac catacaatac ccacgttgca
     6181 tcatcatcac gtgtaatctc aaatggacgt tcatcttcgt taaatccata gtatgcttca
     6241 tcttcttgca tgtcagtttc gtcataaaga agaaactctt ctgtattggc taataattta
     6301 gccgtcgcat ccataaggtt ctctagtcct tgatgtgcta aacttgaaat tggaaaaatc
     6361 ataggcatgt catcaaactc atcatagttt gcagctaatt tttctttgaa tgctgccaaa
     6421 ttctcttctg aatcaggcat atccatttta ttggcaacaa taatttgcgg acgttccatc
     6481 aatcgaagat tgtaagtttc caactcatta ttaattgaaa tatagtcatc gtaagggtct
     6541 cgtccttcac ttgccgacat atcaataaca tgtagaataa cacgtgtacg ctcaatatgt
     6601 ctaaggaact gagttcctaa tccgacacct tgactagccc cttcaatcaa accagggaga
     6661 tcagccattg caaagctatc acctgatttt gtacgaacca tccctaaatt agggacaatt
     6721 gtcgtaaaat gataggcacc aataatccat atgactagta gatcct
//
