LOCUS       V005377                12577 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005377
VERSION     V005377
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 12577)
  AUTHORS   Kornacki JA.
  TITLE     A family of broad-host-range vectors for expression of cloned genes
            in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12577)
  AUTHORS   Xu K, Figurski DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2012) Microbiology and Immunology, Columbia
            University, 701W, 168th Street, Room 1514A, NYC, NY 10027, USA
REFERENCE   3  (bases 1 to 12577)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12577)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (12-JUL-2012) Microbiology and Immunology, Columbia University,
            701W, 168th Street, Room 1514A, NYC, NY 10027, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..12577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      121..207
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      299..326
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        345..436
                     /label="AmpR promoter"
     CDS             2218..2856
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     rep_origin      complement(5153..5547)
                     /direction=LEFT
                     /label="RSF1010 oriV"
                     /note="replication origin of the broad-host-range plasmid
                     RSF1010; requires the RSF1010 RepA/B/C proteins for
                     replication (Scholz et al., 1989)"
     oriT            5889..5976
                     /label="RSF1010 oriT"
                     /note="origin of transfer of the broad-host-range plasmid
                     RSF1010 (Scholz et al., 1989)"
     CDS             7215..8183
                     /label="RSF1010 RepB"
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8697..9533
                     /label="RSF1010 RepA"
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             9523..10371
                     /label="RSF1010 RepC"
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      10535..10565
                     /label="P5 promoter"
                     /note="P5 promoter"
                     /regulatory_class="promoter"
     CDS             complement(10794..11609)
                     /codon_start=1
                     /product="LacI"
                     /label="LacI"
                     /note="represses the expression of downstream lacZ-alpha
                     gene and relives the repression when IPTG is added"
                     /protein_id="AFS32163.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"
     promoter        complement(11733..11810)
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        12040..12068
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    12076..12092
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        12148..12166
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(12179..12286)
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(12295..12313)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12323..12339)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    12528..12545
                     /note="pBAD_rev_primer; pTrcHis_rev_primer"
ORIGIN
        1 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
       61 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
      121 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      181 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
      241 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
      301 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
      361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      601 tgagagtttt cgccccgaat aacgttttcc aatgatgagc acttttagct ttatgcttgt
      661 aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga cctctagggt ccccaattaa
      721 ttagtaatat aatctattaa aggtcattca aaaggtcatc caccggatca attcccctgc
      781 tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt ggagcccttg
      841 ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa
      901 ccctcactga tccgcacatc gggatcactt gttcggtgag gtagcagtcg aagtcgcatt
      961 gcagcgcacg cttgaggaac ggggtgaggc tggtgccttc ggtggagcgc caccgcgcca
     1021 tgacctggat gcaacgcctc aagcgagtct tcaatattga tattgaagtc tgcgaacact
     1081 gcggcggtca cgtcaaagtg attgccagca tcgaagatcc gaaggtcatt gagcagattc
     1141 tcaagcatct gaaacagaaa acagccaagg cgaatgccgc caagcagcgt gagctgccac
     1201 cagaacgagc gccaccgcgc catgacctgg atgcaacgcc tcaagcgagt cttcaatatt
     1261 gatattgaag tctgcgaaca ctgcggcggt cacgtcaaag tgattgccag catcgaagat
     1321 ccgaaggtca ttgagcagat tctcaagcat ctgaaacaga aaacagccaa ggcgaatgcc
     1381 gccaagcagc gtgagctgcc accagaacga gcgccgccac tgactcccag cctgttcgat
     1441 ccatcacaga gtcgtctctt tgactgacga ccccaaatcc aacactgctc aacactgcca
     1501 acttttaaac ggggcggtgg ggcagtttgt atctctcgag ctatcaggct agagatttta
     1561 ccgccaaatc gaaccttatt agagcggttt aggctggacc ggcagttaaa attggggctt
     1621 gagcggtaaa cgagtgaggg aatttcaggt aagatacttc ggatgaggag caaaaaggtg
     1681 gtttatactt cctataccct ttatacttcc tataccccgg ttatccggtg gttttctgtt
     1741 tgcggaataa aacagattag tgacagtttt ctcagaatgg cgtgttccgg tgacttctgt
     1801 tttcgtgtgc tgcatccgga agtgtcaggc ccgccggata tgaaaaaggc ctccggagag
     1861 taccgtgact ttattttcgg cacaaataca ggggtcgatg gataaatacg gcgatagttt
     1921 cctgacggat gatccgtatg taccggcgga agacaagctg caaacctgtc agatggagat
     1981 tgatttaatg gcggatgtgc tgagagcacc gccccgtgaa tccgcagaac tgatccgcta
     2041 tgtgtttgcg gatgattggc cggaataaat aaagccgggc ttaatacaga ttaagcccgt
     2101 atagggtatt attactgaat accaaacagc ttacggagga cggaatgtta cccattgaga
     2161 caaccagact gccttctgat tattaatatt tttcactatt aatcagaagg aataaccatg
     2221 aattttaccc ggattgacct gaatacctgg aatcgcaggg aacactttgc cctttatcgt
     2281 cagcagatta aatgcggatt cagcctgacc accaaactcg atattaccgc tttgcgtacc
     2341 gcactggcgg agacaggtta taagttttat ccgctgatga tttacctgat ctcccgggct
     2401 gttaatcagt ttccggagtt ccggatggca ctgaaagaca atgaacttat ttactgggac
     2461 cagtcagacc cggtctttac tgtctttcat aaagaaaccg aaacattctc tgcactgtcc
     2521 tgccgttatt ttccggatct cagtgagttt atggcaggtt ataatgcggt aacggcagaa
     2581 tatcagcatg ataccagatt gtttccgcag ggaaatttac cggagaatca cctgaatata
     2641 tcatcattac cgtgggtgag ttttgacgga tttaacctga acatcaccgg aaatgatgat
     2701 tattttgccc cggtttttac gatggcaaag tttcagcagg aaggtgaccg cgtattatta
     2761 cctgtttctg tacaggttca tcatgcagtc tgtgatggct ttcatgcagc acggtttatt
     2821 aatacacttc agctgatgtg tgataacata ctgaaataaa ttaattaatt ctgtatttaa
     2881 gccaccgtat ccggcagaat ggtggctttt ttttatattt tacccgtaat ctgtaatttc
     2941 gtttcagaat ggttcaggat cactgtacga taatgccccc gcagtttggt aataccctta
     3001 ataaaaaaga aacagcaaag actgacagca ataataataa agtaagcagt aacaataata
     3061 ttaacaacac cagatgcagt tataataata gtatttaaga caccagaaag actgctgcga
     3121 cagtcatttt gaacaacacc aaaatgccgt aaaggcagta gtaacaacac cagtgaaaac
     3181 atcacgatag catagtgata tgcctgagtg tgtgtaatta aacaataaat aaaccgccat
     3241 atataacaga agatagtatt ctgaatggca tgcttttctg ttcagtataa acatatcatc
     3301 ccggttggta taaggatgat atataataag ttaagctgaa cacatattta ttttggtttt
     3361 attttacaaa taaagtaaga cgatccgtta agtcaaagcg gggtatattt attataccct
     3421 gcttttttat ttgtccgccg ggcgcggata atggatcaga ttatgcagtg tcacaatggc
     3481 cttaccggga ttggcgtaag cgtgcgggat atccgcatgg aagcgcaggg attccccggc
     3541 agaaacggtg tgccactcat cccccagccg cagttgtaat gcgccttcca gtacaatgac
     3601 atgttctctg gttctgaaat ccatccctgt cggtgtggct tatgcagtct ggtcgggact
     3661 cggcgtcgtc ataattacag ccattgcctg gttgtttcat gggcaaaagc tgttggaaga
     3721 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat
     3781 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     3841 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     3901 taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg
     3961 caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc
     4021 tgatccggtg gatgaccttt tgaatgacct ttaatagatt atattactaa ttaattgggg
     4081 accctagagg tccccttttt tattttaaaa attttttcac aaaacggttt acaagcataa
     4141 agctaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     4201 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     4261 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     4321 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4381 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4441 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     4501 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4561 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4621 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4681 agatggtaag ccctcccgct gattaagcat tggtaactgt cagaccaagt ttactcatat
     4741 atactttaga ttgatttctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa
     4801 cccgtagaaa gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca
     4861 catagcccgg caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact
     4921 cgtcaacgat ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat
     4981 cccgatcccg gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac
     5041 tgtgtttaca tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct
     5101 gccgttgcta gacattgcca gccagtgccc gtcactcccg tactaactgt cacgaacccc
     5161 tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa cccctgcaat aactgtcacg
     5221 cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg ggtgttggaa aaatccatcc
     5281 atgattatct aagaataatc cactaggcgc ggttatcagc gcccttgtgg ggcgctgctg
     5341 cccttgccca atatgcccgg ccagaggccg gatagctggt ctattcgctg cgctaggcta
     5401 cacaccgccc caccgctgcg cggcaggggg aaaggcgggc aaagcccgct aaaccccaca
     5461 ccaaaccccg cagaaatacg ctggagcgct tttagccgct ttagcggcct ttccccctac
     5521 ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt ctcggtcgat cattcagccc
     5581 ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc ggtcgtggtc gcgttcaagg
     5641 tacgcatcca ttgccgccat gagccgatcc tccggccact cgctgctgtt caccttggcc
     5701 aaaatcatgg cccccaccag caccttgcgc cttgtttcgt tcttgcgctc ttgctgctgt
     5761 tcccttgccc gctcccgctg aatttcggca ttgattcgcg ctcgttgttc ttcgagcttg
     5821 gccagccgat ccgccgcctt gttgctcccc ttaaccatct tgacacccca ttgttaatgt
     5881 gctgtctcgt aggctatcat ggaggcacag cggcggcaat cccgacccta ctttgtaggg
     5941 gagggcgcac ttaccggttt ctcttcgaga aactggccta acggccaccc ttcgggcggt
     6001 gcgctctccg agggccattg catggagccg aaaagcaaaa gcaacagcga ggcagcatgg
     6061 cgatttatca ccttacggcg aaaaccggca gcaggtcggg cggccaatcg gccagggcca
     6121 aggccgacta catccagcgc gaaggcaagt atgcccgcga catggatgaa gtcttgcacg
     6181 ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc cgactactgg gatgctgccg
     6241 acctgtatga acgcgccaat gggcggctgt tcaaggaggt cgaatttgcc ctgccggtcg
     6301 agctgaccct cgaccagcag aaggcgctgg cgtccgagtt cgcccagcac ctgaccggtg
     6361 ccgagcgcct gccgtatacg ctggccatcc atgccggtgg cggcgagaac ccgcactgcc
     6421 acctgatgat ctccgagcgg atcaatgacg gcatcgagcg gcccgccgct cagtggttca
     6481 agcggtacaa cggcaagacc ccggagaagg gcggggcaca gaagaccgaa gcgctcaagc
     6541 ccaaggcatg gcttgagcag acccgcgagg catgggccga ccatgccaac cgggcattag
     6601 agcgggctgg ccacgacgcc cgcattgacc acagaacact tgaggcgcag ggcatcgagc
     6661 gcctgcccgg tgttcacctg gggccgaacg tggtggagat ggaaggccgg ggcatccgca
     6721 ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa cgcccagatc atcgacttac
     6781 aggaataccg ggaggcaata gaccatgaac gcaatcgaca gagtgaagaa atccagaggc
     6841 atcaacgagt tagcggagca gatcgaaccg ctggcccaga gcatggcgac actggccgac
     6901 gaagcccggc aggtcatgag ccagaccaag caggccagcg aggcgcaggc ggcggagtgg
     6961 ctgaaagccc agcgccagac aggggcggca tgggtggagc tggccaaaga gttgcgggag
     7021 gtagccgccg aggtgagcag cgccgcgcag agcgcccgga gcgcgtcgcg ggggtggcac
     7081 tggaagctat ggctaaccgt gatgctggct tccatgatgc ctacggtggt gctgctgatc
     7141 gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg aggacggctc gatctggctg
     7201 cgcttggtgg cccgatgaag aacgacagga ctttgcaggc cataggccga cagctcaagg
     7261 ccatgggctg tgagcgcttc gatatcggcg tcagggacgc acccaccggc cagatgatga
     7321 accgggaatg gtcagccgcc gaagtgctcc agaacacgcc atggctcaag cggatgaatg
     7381 cccagggcaa tgacgtgtat atcaggcccg ccgagcagga gcggcatggt ctggtgctgg
     7441 tggacgacct cagcgagttt gacctggatg acatgaaagc cgagggccgg gagcctgccc
     7501 tggtagtgga aaccagcccg aagaactatc aggcatgggt caaggtggcc gacgccgcag
     7561 gcggtgaact tcgggggcag attgcccgga cgctggccag cgagtacgac gccgacccgg
     7621 ccagcgccga cagccgccac tatggccgct tggcgggctt caccaaccgc aaggacaagc
     7681 acaccacccg cgccggttat cagccgtggg tgctgctgcg tgaatccaag ggcaagaccg
     7741 ccaccgctgg cccggcgctg gtgcagcagg ctggccagca gatcgagcag gcccagcggc
     7801 agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc cgagcggcag cttagccgcc
     7861 accggcgcac ggcgctggac gagtaccgca gcgagatggc cgggctggtc aagcgcttcg
     7921 gtcatgacct cagcaagtgc gactttatcg ccgcgcagaa gctggccagc cggggccgca
     7981 gtgccgagga aatcggcaag gccatggccg aggccagccc agcgctggca gagcgcaagc
     8041 ccggccacga agcggattac atcgagcgca ccgtcagcaa ggtcatgggt ctgcccagcg
     8101 tccagcttgc gcgggccgag ctggcacggg caccggcacc ccgccagcga ggcatggaca
     8161 ggggcgggcc agatttcagc atgtagtgct tgcgttggta ctcacgcctg ttatactatg
     8221 agtactcacg cacagaaggg ggttttatgg aatacgaaaa aagcgcttca gggtcggtct
     8281 acctgatcaa aagtgacaag ggctattggt tgcccggtgg ctttggttat acgtcaaaca
     8341 aggccgaggc tggccgcttt tcagtcgctg atatggccag ccttaacctt gacggctgca
     8401 ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg caagtttctc ggtgactgat
     8461 atgaaagacc aaaaggacaa gcagaccggc gacctgctgg ccagccctga cgctgtacgc
     8521 caagcgcgat atgccgagcg catgaaggcc aaagggatgc gtcagcgcaa gttctggctg
     8581 accgacgacg aatacgaggc gctgcgcgag tgcctggaag aactcagagc ggcgcagggc
     8641 gggggtagtg accccgccag cgcctaacca ccaactgcct gcaaaggagg caatcaatgg
     8701 ctacccataa gcctatcaat attctggagg cgttcgcagc agcgccgcca ccgctggact
     8761 acgttttgcc caacatggtg gccggtacgg tcggggcgct ggtgtcgccc ggtggtgccg
     8821 gtaaatccat gctggccctg caactggccg cacagattgc aggcgggccg gatctgctgg
     8881 aggtgggcga actgcccacc ggcccggtga tctacctgcc cgccgaagac ccgcccaccg
     8941 ccattcatca ccgcctgcac gcccttgggg cgcacctcag cgccgaggaa cggcaagccg
     9001 tggctgacgg cctgctgatc cagccgctga tcggcagcct gcccaacatc atggccccgg
     9061 agtggttcga cggcctcaag cgcgccgccg agggccgccg cctgatggtg ctggacacgc
     9121 tgcgccggtt ccacatcgag gaagaaaacg ccagcggccc catggcccag gtcatcggtc
     9181 gcatggaggc catcgccgcc gataccgggt gctctatcgt gttcctgcac catgccagca
     9241 agggcgcggc catgatgggc gcaggcgacc agcagcaggc cagccggggc agctcggtac
     9301 tggtcgataa catccgctgg cagtcctacc tgtcgagcat gaccagcgcc gaggccgagg
     9361 aatggggtgt ggacgacgac cagcgccggt tcttcgtccg cttcggtgtg agcaaggcca
     9421 actatggcgc accgttcgct gatcggtggt tcaggcggca tgacggcggg gtgctcaagc
     9481 ccgccgtgct ggagaggcag cgcaagagca agggggtgcc ccgtggtgaa gcctaagaac
     9541 aagcacagcc tcagccacgt ccggcacgac ccggcgcact gtctggcccc cggcctgttc
     9601 cgtgccctca agcggggcga gcgcaagcgc agcaagctgg acgtgacgta tgactacggc
     9661 gacggcaagc ggatcgagtt cagcggcccg gagccgctgg gcgctgatga tctgcgcatc
     9721 ctgcaagggc tggtggccat ggctgggcct aatggcctag tgcttggccc ggaacccaag
     9781 accgaaggcg gacggcagct ccggctgttc ctggaaccca agtgggaggc cgtcaccgct
     9841 gaatgccatg tggtcaaagg tagctatcgg gcgctggcaa aggaaatcgg ggcagaggtc
     9901 gatagtggtg gggcgctcaa gcacatacag gactgcatcg agcgcctttg gaaggtatcc
     9961 atcatcgccc agaatggccg caagcggcag gggtttcggc tgctgtcgga gtacgccagc
    10021 gacgaggcgg acgggcgcct gtacgtggcc ctgaacccct tgatcgcgca ggccgtcatg
    10081 ggtggcggcc agcatgtgcg catcagcatg gacgaggtgc gggcgctgga cagcgaaacc
    10141 gcccgcctgc tgcaccagcg gctgtgtggc tggatcgacc ccggcaaaac cggcaaggct
    10201 tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg ccagtggttc gaccatgcgc
    10261 aagcgccgca agcgggtgcg cgaggcgttg ccggagctgg tcgcgctggg ctggacggta
    10321 accgagttcg cggcgggcaa gtacgacatc acccggccca aggcggcagg ctgacccccc
    10381 ccactctatt gtaaacaaga catttttatc ttttatattc aatggcttat tttcctgcta
    10441 attggtaata ccatgaaaaa taccatgctc agaaaaggct taacaatatt ttgaaaaatt
    10501 gcctactgag cgctgccgca cagctccata ggccgctttc ctggctttgc ttccagatgt
    10561 atgctcttct gctcccgaac gccagcaaga cgtagcccag cgcgtcggcc agcttgcaat
    10621 tcgcgctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10681 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10741 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
    10801 ctggccctga gagagttgca gcaagcggtc cacgtggttt gccccagcag gcgaaaatcc
    10861 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
    10921 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
    10981 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
    11041 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
    11101 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
    11161 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
    11221 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
    11281 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
    11341 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
    11401 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
    11461 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc
    11521 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg
    11581 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca
    11641 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac
    11701 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc
    11761 gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg
    11821 ccgcttcgcc ttcgcgcgcg aattgcaagc tgatccgggc ttatcgactg cacggtgcac
    11881 caatgcttct ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat
    11941 cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg
    12001 acatcataac ggttctggca aatattctga aatgagctgt tgacaattaa tcatcggctc
    12061 gtataatgtg tggaattgtg agcggataac aatttcacac aggaattata aaggaaacag
    12121 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctggg
    12181 taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc
    12241 cgggggatcc actagttcta gagcggccgc caccgcggtg gagctccaat tcgccctata
    12301 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
    12361 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
    12421 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga
    12481 aattgtaagt gcacaaagtg cggtcgcgga tgagagaaga ttttcagcct gatacagatt
    12541 aaatcagaac gcagaagcgg tctgataaaa cagaatt
//
