LOCUS       40924_25866       11285 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAK14-Blue, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Kornacki JA.
  TITLE     A family of broad-host-range vectors for expression of cloned genes 
            in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11285)
  AUTHORS   Xu K, Figurski DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2012) Microbiology and Immunology, Columbia 
            University, 701W, 168th Street, Room 1514A, NYC, NY 10027, USA
REFERENCE   3  (bases 1 to 11285)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11285)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JUL-2012) Microbiology and Immunology, Columbia University, 
            701W, 168th Street, Room 1514A, NYC, NY 10027, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      121..207
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      299..326
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        345..436
                     /label=AmpR promoter
     CDS             1446..2237
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(3861..4255)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4597..4684
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5923..6891
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7405..8241
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8231..9079
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      9244..9274
                     /label=P5 promoter
                     /note="P5 promoter"
                     /regulatory_class="promoter"
     CDS             complement(9502..10317)
                     /codon_start=1
                     /product="LacI"
                     /label=LacI
                     /note="represses the expression of downstream lacZ-alpha
                     gene and relives the repression when IPTG is added"
                     /protein_id="AFS32161.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"
     promoter        complement(10441..10518)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        10748..10776
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10784..10800
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10856..10874
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(10887..10994)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(11003..11021)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11031..11047)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11237..11254
                     /note="pBAD_rev_primer; pTrcHis_rev_primer"
ORIGIN
        1 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
       61 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
      121 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      181 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
      241 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
      301 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
      361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      601 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttagct ttatgcttgt
      661 aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga cctctagggt ccccaattaa
      721 ttagtaatat aatctattaa aggtcattca aaaggtcatc caccggatca gcttagtaaa
      781 gccctcgcta gattttaatg cggatgttgc gattacttcg ccaactattg cgataacaag
      841 aaaaagccag cctttcatga tatatctccc aatttgtgta gggcttatta tgcacgctta
      901 aaaataataa aagcagactt gacctgatag tttggctgtg agcaattatg tgcttagtgc
      961 atctaacgct tgagttaagc cgcgccgcga agcggcgtcg gcttgaacga attgttagac
     1021 attatttgcc gactaccttg gtgatctcgc ctttcacgca ccggacaaaa tcttcccact
     1081 gatctgcgcg agcttcacac tggngcaagc nctcagggcg caagggctgc taaaggaagc
     1141 ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
     1201 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
     1261 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
     1321 ctggggcgcc ctctggtaag gntgggaagc cctgcaaagt aaactggatg gctttcttgc
     1381 cgccaaggat ctgatggcgc angggatcaa gatctgatca agagacagga tgangatcgt
     1441 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc
     1501 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc
     1561 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg
     1621 aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag
     1681 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg
     1741 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg
     1801 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac
     1861 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg
     1921 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc
     1981 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg
     2041 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc
     2101 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc
     2161 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc
     2221 ttcttgacga gttcttctga gcgggactct ggggttcgan atgaccgacc aagcgacgcc
     2281 caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggn tgggcttcgg
     2341 aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggaatctc angctggagn
     2401 tcttcgncca cnccgggntc gatcccctcg cgagttggtt cagctgctgc ctgaggctgg
     2461 acgacctcgc ggagttctac cggcagtgca aatccgtcgg catccaggaa accagcagcg
     2521 gctatccgcg catccatgcc cccgaactgc aggagtgggg aggcacgatg gccgctttgg
     2581 tcgacggatc agtgagggtt tgcaactgcg ggtcaaggat ctggatttcg atcacggcac
     2641 gatcatcgtg cgggagggca agggctccaa ggatcgggcc ttgatgttac ccgagagctt
     2701 ggcacccagc ctgcgcgagc aggggaattg atccggtgga tgaccttttg aatgaccttt
     2761 aatagattat attactaatt aattggggac cctagaggtc ccctttttta ttttaaaaat
     2821 tttttcacaa aacggtttac aagcataaag ctaaagttct gctatgtggc gcggtattat
     2881 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     2941 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcangata gtaagagaat
     3001 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     3061 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     3121 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     3181 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     3241 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     3301 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     3361 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgctga ttaagcattg
     3421 gtaactgtca gaccaagttt actcatatat actttagatt gatttctgaa agcgaccagg
     3481 tgctcggcgt ggcaagactc gcagcgaacc cgtagaaagc catgctccag ccgcccgcat
     3541 tggagaaatt cttcaaattc ccgttgcaca tagcccggca attcctttcc ctgctctgcc
     3601 ataagcgcag cgaatgccgg gtaatactcg tcaacgatct gatagagaag ggtttgctcg
     3661 ggtcggtggc tctggtaacg accagtatcc cgatcccggc tggccgtcct ggccgccaca
     3721 tgaggcatgt tccgcgtcct tgcaatactg tgtttacata cagtctatcg cttagcggaa
     3781 agttctttta ccctcagccg aaatgcctgc cgttgctaga cattgccagc cagtgcccgt
     3841 cactcccgta ctaactgtca cgaacccctg caataactgt cacgcccccc tgcaataact
     3901 gtcacgaacc cctgcaataa ctgtcacgcc cccaaacctg caaacccagc aggggcgggg
     3961 gctggcgggg tgttggaaaa atccatccat gattatctaa gaataatcca ctaggcgcgg
     4021 ttatcagcgc ccttgtgggg cgctgctgcc cttgcccaat atgcccggcc agaggccgga
     4081 tagctggtct attcgctgcg ctaggctaca caccgcccca ccgctgcgcg gcagggggaa
     4141 aggcgggcaa agcccgctaa accccacacc aaaccccgca gaaatacgct ggagcgcttt
     4201 tagccgcttt agcggccttt ccccctaccc gaagggtggg ggcgcgtgtg cagccccgca
     4261 gggcctgtct cggtcgatca ttcagcccgg ctcatccttc tggcgtggcg gcagaccgaa
     4321 caaggcgcgg tcgtggtcgc gttcaaggta cgcatccatt gccgccatga gccgatcctc
     4381 cggccactcg ctgctgttca ccttggccaa aatcatggcc cccaccagca ccttgcgcct
     4441 tgtttcgttc ttgcgctctt gctgctgttc ccttgcccgc tcccgctgaa tttcggcatt
     4501 gattcgcgct cgttgttctt cgagcttggc cagccgatcc gccgccttgt tgctcccctt
     4561 aaccatcttg acaccccatt gttaatgtgc tgtctcgtag gctatcatgg aggcacagcg
     4621 gcggcaatcc cgaccctact ttgtagggga gggcgcactt accggtttct cttcgagaaa
     4681 ctggcctaac ggccaccctt cgggcggtgc gctctccgag ggccattgca tggagccgaa
     4741 aagcaaaagc aacagcgagg cagcatggcg atttatcacc ttacggcgaa aaccggcagc
     4801 aggtcgggcg gccaatcggc cagggccaag gccgactaca tccagcgcga aggcaagtat
     4861 gcccgcgaca tggatgaagt cttgcacgcc gaatccgggc acatgccgga gttcgtcgag
     4921 cggcccgccg actactggga tgctgccgac ctgtatgaac gcgccaatgg gcggctgttc
     4981 aaggaggtcg aatttgccct gccggtcgag ctgaccctcg accagcagaa ggcgctggcg
     5041 tccgagttcg cccagcacct gaccggtgcc gagcgcctgc cgtatacgct ggccatccat
     5101 gccggtggcg gcgagaaccc gcactgccac ctgatgatct ccgagcggat caatgacggc
     5161 atcgagcggc ccgccgctca gtggttcaag cggtacaacg gcaagacccc ggagaagggc
     5221 ggggcacaga agaccgaagc gctcaagccc aaggcatggc ttgagcagac ccgcgaggca
     5281 tgggccgacc atgccaaccg ggcattagag cgggctggcc acgacgcccg cattgaccac
     5341 agaacacttg aggcgcaggg catcgagcgc ctgcccggtg ttcacctggg gccgaacgtg
     5401 gtggagatgg aaggccgggg catccgcacc gaccgggcag acgtggccct gaacatcgac
     5461 accgccaacg cccagatcat cgacttacag gaataccggg aggcaataga ccatgaacgc
     5521 aatcgacaga gtgaagaaat ccagaggcat caacgagtta gcggagcaga tcgaaccgct
     5581 ggcccagagc atggcgacac tggccgacga agcccggcag gtcatgagcc agaccaagca
     5641 ggccagcgag gcgcaggcgg cggagtggct gaaagcccag cgccagacag gggcggcatg
     5701 ggtggagctg gccaaagagt tgcgggaggt agccgccgag gtgagcagcg ccgcgcagag
     5761 cgcccggagc gcgtcgcggg ggtggcactg gaagctatgg ctaaccgtga tgctggcttc
     5821 catgatgcct acggtggtgc tgctgatcgc atcgttgctc ttgctcgacc tgacgccact
     5881 gacaaccgag gacggctcga tctggctgcg cttggtggcc cgatgaagaa cgacaggact
     5941 ttgcaggcca taggccgaca gctcaaggcc atgggctgtg agcgcttcga tatcggcgtc
     6001 agggacgcac ccaccggcca gatgatgaac cgggaatggt cagccgccga agtgctccag
     6061 aacacgccat ggctcaagcg gatgaatgcc cagggcaatg acgtgtatat caggcccgcc
     6121 gagcaggagc ggcatggtct ggtgctggtg gacgacctca gcgagtttga cctggatgac
     6181 atgaaagccg agggccggga gcctgccctg gtagtggaaa ccagcccgaa gaactatcag
     6241 gcatgggtca aggtggccga cgccgcaggc ggtgaacttc gggggcagat tgcccggacg
     6301 ctggccagcg agtacgacgc cgacccggcc agcgccgaca gccgccacta tggccgcttg
     6361 gcgggcttca ccaaccgcaa ggacaagcac accacccgcg ccggttatca gccgtgggtg
     6421 ctgctgcgtg aatccaaggg caagaccgcc accgctggcc cggcgctggt gcagcaggct
     6481 ggccagcaga tcgagcaggc ccagcggcag caggagaagg cccgcaggct ggccagcctc
     6541 gaactgcccg agcggcagct tagccgccac cggcgcacgg cgctggacga gtaccgcagc
     6601 gagatggccg ggctggtcaa gcgcttcggt catgacctca gcaagtgcga ctttatcgcc
     6661 gcgcagaagc tggccagccg gggccgcagt gccgaggaaa tcggcaaggc catggccgag
     6721 gccagcccag cgctggcaga gcgcaagccc ggccacgaag cggattacat cgagcgcacc
     6781 gtcagcaagg tcatgggtct gcccagcgtc cagcttgcgc gggccgagct ggcacgggca
     6841 ccggcacccc gccagcgagg catggacagg ggcgggccag atttcagcat gtagtgcttg
     6901 cgttggtact cacgcctgtt atactatgag tactcacgca cagaaggggg ttttatggaa
     6961 tacgaaaaaa gcgcttcagg gtcggtctac ctgatcaaaa gtgacaaggg ctattggttg
     7021 cccggtggct ttggttatac gtcaaacaag gccgaggctg gccgcttttc agtcgctgat
     7081 atggccagcc ttaaccttga cggctgcacc ttgtccttgt tccgcgaaga caagcctttc
     7141 ggccccggca agtttctcgg tgactgatat gaaagaccaa aaggacaagc agaccggcga
     7201 cctgctggcc agccctgacg ctgtacgcca agcgcgatat gccgagcgca tgaaggccaa
     7261 agggatgcgt cagcgcaagt tctggctgac cgacgacgaa tacgaggcgc tgcgcgagtg
     7321 cctggaagaa ctcagagcgg cgcagggcgg gggtagtgac cccgccagcg cctaaccacc
     7381 aactgcctgc aaaggaggca atcaatggct acccataagc ctatcaatat tctggaggcg
     7441 ttcgcagcag cgccgccacc gctggactac gttttgccca acatggtggc cggtacggtc
     7501 ggggcgctgg tgtcgcccgg tggtgccggt aaatccatgc tggccctgca actggccgca
     7561 cagattgcag gcgggccgga tctgctggag gtgggcgaac tgcccaccgg cccggtgatc
     7621 tacctgcccg ccgaagaccc gcccaccgcc attcatcacc gcctgcacgc ccttggggcg
     7681 cacctcagcg ccgaggaacg gcaagccgtg gctgacggcc tgctgatcca gccgctgatc
     7741 ggcagcctgc ccaacatcat ggccccggag tggttcgacg gcctcaagcg cgccgccgag
     7801 ggccgccgcc tgatggtgct ggacacgctg cgccggttcc acatcgagga agaaaacgcc
     7861 agcggcccca tggcccaggt catcggtcgc atggaggcca tcgccgccga taccgggtgc
     7921 tctatcgtgt tcctgcacca tgccagcaag ggcgcggcca tgatgggcgc aggcgaccag
     7981 cagcaggcca gccggggcag ctcggtactg gtcgataaca tccgctggca gtcctacctg
     8041 tcgagcatga ccagcgccga ggccgaggaa tggggtgtgg acgacgacca gcgccggttc
     8101 ttcgtccgct tcggtgtgag caaggccaac tatggcgcac cgttcgctga tcggtggttc
     8161 aggcggcatg acggcggggt gctcaagccc gccgtgctgg agaggcagcg caagagcaag
     8221 ggggtgcccc gtggtgaagc ctaagaacaa gcacagcctc agccacgtcc ggcacgaccc
     8281 ggcgcactgt ctggcccccg gcctgttccg tgccctcaag cggggcgagc gcaagcgcag
     8341 caagctggac gtgacgtatg actacggcga cggcaagcgg atcgagttca gcggcccgga
     8401 gccgctgggc gctgatgatc tgcgcatcct gcaagggctg gtggccatgg ctgggcctaa
     8461 tggcctagtg cttggcccgg aacccaagac cgaaggcgga cggcagctcc ggctgttcct
     8521 ggaacccaag tgggaggccg tcaccgctga atgccatgtg gtcaaaggta gctatcgggc
     8581 gctggcaaag gaaatcgggg cagaggtcga tagtggtggg gcgctcaagc acatacagga
     8641 ctgcatcgag cgcctttgga aggtatccat catcgcccag aatggccgca agcggcaggg
     8701 gtttcggctg ctgtcggagt acgccagcga cgaggcggac gggcgcctgt acgtggccct
     8761 gaaccccttg atcgcgcagg ccgtcatggg tggcggccag catgtgcgca tcagcatgga
     8821 cgaggtgcgg gcgctggaca gcgaaaccgc ccgcctgctg caccagcggc tgtgtggctg
     8881 gatcgacccc ggcaaaaccg gcaaggcttc catagatacc ttgtgcggct atgtctggcc
     8941 gtcagaggcc agtggttcga ccatgcgcaa gcgccgcaag cgggtgcgcg aggcgttgcc
     9001 ggagctggtc gcgctgggct ggacggtaac cgagttcgcg gcgggcaagt acgacatcac
     9061 ccggcccaag gcggcaggct gacccccccc actctattgt aaacaagaca tttttatctt
     9121 ttatattcaa tggcttattt tcctgctaat tggtaatacc atgaaaaata ccatgctcag
     9181 aaaaggctta acaatatttt gaaaaattgc ctactgagcg ctgccgcaca gctccatagg
     9241 ccgctttcct ggctttgctt ccagatgtat gctcttctgc tcccgaacgc cagcaagacg
     9301 tagcccagcg cgtcggccag cttgcaattc gcgctaactt acattaattg cgttgcgctc
     9361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9421 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga
     9481 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     9541 cgtggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
     9601 tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
     9661 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
     9721 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
     9781 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
     9841 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
     9901 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt catgggagaa
     9961 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
    10021 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
    10081 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
    10141 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
    10201 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
    10261 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
    10321 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
    10381 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
    10441 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
    10501 gcaccattcg atggtgtcaa cgtaaatgcc gcttcgcctt cgcgcgcgaa ttgcaagctg
    10561 atccgggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag
    10621 ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact
    10681 cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa
    10741 tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa
    10801 tttcacacag gaattataaa ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    10861 accctcacta aagggaacaa aagctgggta ccgggccccc cctcgaggtc gacggtatcg
    10921 ataagcttga tatcgaattc ctgcagcccg ggggatccac tagttctaga gcggccgcca
    10981 ccgcggtgga gctccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    11041 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    11101 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    11161 cagttgcgca gcctgaatgg cgaatggaaa ttgtaagtgc acaaagtgcg gtcgcggatg
    11221 agagaagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
    11281 gaatt
//
