LOCUS       40924_25861       11097 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAK12-Blue, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Kornacki JA.
  TITLE     A family of broad-host-range vectors for expression of cloned genes 
            in Gram-negative bacteria
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11097)
  AUTHORS   Xu K, Figurski DH.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JUL-2012) Microbiology and Immunology, Columbia 
            University, 701W, 168th Street, Room 1514A, NYC, NY 10027, USA
REFERENCE   3  (bases 1 to 11097)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11097)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JUL-2012) Microbiology and Immunology, Columbia University, 
            701W, 168th Street, Room 1514A, NYC, NY 10027, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11097
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      121..207
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      299..326
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        345..436
                     /label=AmpR promoter
     CDS             1498..2286
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      complement(3673..4067)
                     /direction=LEFT
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            4409..4496
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             5735..6703
                     /label=RSF1010 RepB
                     /note="replication protein B of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             7217..8053
                     /label=RSF1010 RepA
                     /note="replication protein A of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     CDS             8043..8891
                     /label=RSF1010 RepC
                     /note="replication protein C of the broad-host-range
                     plasmid RSF1010 (Scholz et al., 1989)"
     regulatory      9055..9085
                     /label=P5 promoter
                     /note="P5 promoter"
                     /regulatory_class="promoter"
     CDS             complement(9314..10129)
                     /codon_start=1
                     /gene="lacI"
                     /product="LacI"
                     /label=lacI
                     /note="represses the expression of downstream lacZ-alpha
                     gene and relives the repression when IPTG is added"
                     /protein_id="AFS32168.1"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR
                     ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA
                     LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY
                     LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR
                     VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTTWTACCNSLRARR"
     gene            complement(9314..10129)
                     /gene="lacI"
                     /label=lacI
     promoter        complement(10253..10330)
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        10560..10588
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    10596..10612
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        10668..10686
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    complement(10699..10806)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(10815..10833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10843..10859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    11048..11065
                     /note="pBAD_rev_primer; pTrcHis_rev_primer"
ORIGIN
        1 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
       61 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
      121 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      181 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
      241 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
      301 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
      361 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
      421 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
      481 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
      541 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
      601 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttagct ttatgcttgt
      661 aaaccgtttt gtgaaaaaat ttttaaaata aaaaagggga cctctagggt ccccaattaa
      721 ttagtaatat aatctattaa aggtcattca aaaggtcatc caccggatca attcccctgc
      781 tcgcgcaggc tgggtgccaa gctctcgggt aacatcaagg cccgatcctt ggagcccttg
      841 ccctcccgca cgatgatcgt gccgtgatcg aaatccagat ccttgacccg cagttgcaaa
      901 ccctcactga tccgcatgcc cgttccatac agaagctggg cgaacaaacg atgctcgcct
      961 tccagaaaac cgaggatgcg aaccacttca tccggggtca gcaccaccgg caagcgccgc
     1021 gacggccgag gtcttccgat ctcctgaagc cagggcagat ccgtgcacag caccttgccg
     1081 tagaagaaca gcaaggccgc caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg
     1141 ttcgccagcc aggacagaaa tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc
     1201 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     1261 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     1321 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     1381 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     1441 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     1501 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     1561 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     1621 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     1681 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     1741 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     1801 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     1861 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     1921 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     1981 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     2041 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     2101 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     2161 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     2221 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     2281 ggcaaataat gtctaacaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
     2341 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
     2401 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg
     2461 ctggcttttt cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc
     2521 tagcgagggc tttactaagc tgatccggtg gatgaccttt tgaatgacct ttaatagatt
     2581 atattactaa ttaattgggg accctagagg tccccttttt tattttaaaa attttttcac
     2641 aaaacggttt acaagcataa agctaaagtt ctgctatgtg gcgcggtatt atcccgtgtt
     2701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     3001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     3061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3181 atcattgcag cactggggcc agatggtaag ccctcccgct gattaagcat tggtaactgt
     3241 cagaccaagt ttactcatat atactttaga ttgatttctg aaagcgacca ggtgctcggc
     3301 gtggcaagac tcgcagcgaa cccgtagaaa gccatgctcc agccgcccgc attggagaaa
     3361 ttcttcaaat tcccgttgca catagcccgg caattccttt ccctgctctg ccataagcgc
     3421 agcgaatgcc gggtaatact cgtcaacgat ctgatagaga agggtttgct cgggtcggtg
     3481 gctctggtaa cgaccagtat cccgatcccg gctggccgtc ctggccgcca catgaggcat
     3541 gttccgcgtc cttgcaatac tgtgtttaca tacagtctat cgcttagcgg aaagttcttt
     3601 taccctcagc cgaaatgcct gccgttgcta gacattgcca gccagtgccc gtcactcccg
     3661 tactaactgt cacgaacccc tgcaataact gtcacgcccc cctgcaataa ctgtcacgaa
     3721 cccctgcaat aactgtcacg cccccaaacc tgcaaaccca gcaggggcgg gggctggcgg
     3781 ggtgttggaa aaatccatcc atgattatct aagaataatc cactaggcgc ggttatcagc
     3841 gcccttgtgg ggcgctgctg cccttgccca atatgcccgg ccagaggccg gatagctggt
     3901 ctattcgctg cgctaggcta cacaccgccc caccgctgcg cggcaggggg aaaggcgggc
     3961 aaagcccgct aaaccccaca ccaaaccccg cagaaatacg ctggagcgct tttagccgct
     4021 ttagcggcct ttccccctac ccgaagggtg ggggcgcgtg tgcagccccg cagggcctgt
     4081 ctcggtcgat cattcagccc ggctcatcct tctggcgtgg cggcagaccg aacaaggcgc
     4141 ggtcgtggtc gcgttcaagg tacgcatcca ttgccgccat gagccgatcc tccggccact
     4201 cgctgctgtt caccttggcc aaaatcatgg cccccaccag caccttgcgc cttgtttcgt
     4261 tcttgcgctc ttgctgctgt tcccttgccc gctcccgctg aatttcggca ttgattcgcg
     4321 ctcgttgttc ttcgagcttg gccagccgat ccgccgcctt gttgctcccc ttaaccatct
     4381 tgacacccca ttgttaatgt gctgtctcgt aggctatcat ggaggcacag cggcggcaat
     4441 cccgacccta ctttgtaggg gagggcgcac ttaccggttt ctcttcgaga aactggccta
     4501 acggccaccc ttcgggcggt gcgctctccg agggccattg catggagccg aaaagcaaaa
     4561 gcaacagcga ggcagcatgg cgatttatca ccttacggcg aaaaccggca gcaggtcggg
     4621 cggccaatcg gccagggcca aggccgacta catccagcgc gaaggcaagt atgcccgcga
     4681 catggatgaa gtcttgcacg ccgaatccgg gcacatgccg gagttcgtcg agcggcccgc
     4741 cgactactgg gatgctgccg acctgtatga acgcgccaat gggcggctgt tcaaggaggt
     4801 cgaatttgcc ctgccggtcg agctgaccct cgaccagcag aaggcgctgg cgtccgagtt
     4861 cgcccagcac ctgaccggtg ccgagcgcct gccgtatacg ctggccatcc atgccggtgg
     4921 cggcgagaac ccgcactgcc acctgatgat ctccgagcgg atcaatgacg gcatcgagcg
     4981 gcccgccgct cagtggttca agcggtacaa cggcaagacc ccggagaagg gcggggcaca
     5041 gaagaccgaa gcgctcaagc ccaaggcatg gcttgagcag acccgcgagg catgggccga
     5101 ccatgccaac cgggcattag agcgggctgg ccacgacgcc cgcattgacc acagaacact
     5161 tgaggcgcag ggcatcgagc gcctgcccgg tgttcacctg gggccgaacg tggtggagat
     5221 ggaaggccgg ggcatccgca ccgaccgggc agacgtggcc ctgaacatcg acaccgccaa
     5281 cgcccagatc atcgacttac aggaataccg ggaggcaata gaccatgaac gcaatcgaca
     5341 gagtgaagaa atccagaggc atcaacgagt tagcggagca gatcgaaccg ctggcccaga
     5401 gcatggcgac actggccgac gaagcccggc aggtcatgag ccagaccaag caggccagcg
     5461 aggcgcaggc ggcggagtgg ctgaaagccc agcgccagac aggggcggca tgggtggagc
     5521 tggccaaaga gttgcgggag gtagccgccg aggtgagcag cgccgcgcag agcgcccgga
     5581 gcgcgtcgcg ggggtggcac tggaagctat ggctaaccgt gatgctggct tccatgatgc
     5641 ctacggtggt gctgctgatc gcatcgttgc tcttgctcga cctgacgcca ctgacaaccg
     5701 aggacggctc gatctggctg cgcttggtgg cccgatgaag aacgacagga ctttgcaggc
     5761 cataggccga cagctcaagg ccatgggctg tgagcgcttc gatatcggcg tcagggacgc
     5821 acccaccggc cagatgatga accgggaatg gtcagccgcc gaagtgctcc agaacacgcc
     5881 atggctcaag cggatgaatg cccagggcaa tgacgtgtat atcaggcccg ccgagcagga
     5941 gcggcatggt ctggtgctgg tggacgacct cagcgagttt gacctggatg acatgaaagc
     6001 cgagggccgg gagcctgccc tggtagtgga aaccagcccg aagaactatc aggcatgggt
     6061 caaggtggcc gacgccgcag gcggtgaact tcgggggcag attgcccgga cgctggccag
     6121 cgagtacgac gccgacccgg ccagcgccga cagccgccac tatggccgct tggcgggctt
     6181 caccaaccgc aaggacaagc acaccacccg cgccggttat cagccgtggg tgctgctgcg
     6241 tgaatccaag ggcaagaccg ccaccgctgg cccggcgctg gtgcagcagg ctggccagca
     6301 gatcgagcag gcccagcggc agcaggagaa ggcccgcagg ctggccagcc tcgaactgcc
     6361 cgagcggcag cttagccgcc accggcgcac ggcgctggac gagtaccgca gcgagatggc
     6421 cgggctggtc aagcgcttcg gtcatgacct cagcaagtgc gactttatcg ccgcgcagaa
     6481 gctggccagc cggggccgca gtgccgagga aatcggcaag gccatggccg aggccagccc
     6541 agcgctggca gagcgcaagc ccggccacga agcggattac atcgagcgca ccgtcagcaa
     6601 ggtcatgggt ctgcccagcg tccagcttgc gcgggccgag ctggcacggg caccggcacc
     6661 ccgccagcga ggcatggaca ggggcgggcc agatttcagc atgtagtgct tgcgttggta
     6721 ctcacgcctg ttatactatg agtactcacg cacagaaggg ggttttatgg aatacgaaaa
     6781 aagcgcttca gggtcggtct acctgatcaa aagtgacaag ggctattggt tgcccggtgg
     6841 ctttggttat acgtcaaaca aggccgaggc tggccgcttt tcagtcgctg atatggccag
     6901 ccttaacctt gacggctgca ccttgtcctt gttccgcgaa gacaagcctt tcggccccgg
     6961 caagtttctc ggtgactgat atgaaagacc aaaaggacaa gcagaccggc gacctgctgg
     7021 ccagccctga cgctgtacgc caagcgcgat atgccgagcg catgaaggcc aaagggatgc
     7081 gtcagcgcaa gttctggctg accgacgacg aatacgaggc gctgcgcgag tgcctggaag
     7141 aactcagagc ggcgcagggc gggggtagtg accccgccag cgcctaacca ccaactgcct
     7201 gcaaaggagg caatcaatgg ctacccataa gcctatcaat attctggagg cgttcgcagc
     7261 agcgccgcca ccgctggact acgttttgcc caacatggtg gccggtacgg tcggggcgct
     7321 ggtgtcgccc ggtggtgccg gtaaatccat gctggccctg caactggccg cacagattgc
     7381 aggcgggccg gatctgctgg aggtgggcga actgcccacc ggcccggtga tctacctgcc
     7441 cgccgaagac ccgcccaccg ccattcatca ccgcctgcac gcccttgggg cgcacctcag
     7501 cgccgaggaa cggcaagccg tggctgacgg cctgctgatc cagccgctga tcggcagcct
     7561 gcccaacatc atggccccgg agtggttcga cggcctcaag cgcgccgccg agggccgccg
     7621 cctgatggtg ctggacacgc tgcgccggtt ccacatcgag gaagaaaacg ccagcggccc
     7681 catggcccag gtcatcggtc gcatggaggc catcgccgcc gataccgggt gctctatcgt
     7741 gttcctgcac catgccagca agggcgcggc catgatgggc gcaggcgacc agcagcaggc
     7801 cagccggggc agctcggtac tggtcgataa catccgctgg cagtcctacc tgtcgagcat
     7861 gaccagcgcc gaggccgagg aatggggtgt ggacgacgac cagcgccggt tcttcgtccg
     7921 cttcggtgtg agcaaggcca actatggcgc accgttcgct gatcggtggt tcaggcggca
     7981 tgacggcggg gtgctcaagc ccgccgtgct ggagaggcag cgcaagagca agggggtgcc
     8041 ccgtggtgaa gcctaagaac aagcacagcc tcagccacgt ccggcacgac ccggcgcact
     8101 gtctggcccc cggcctgttc cgtgccctca agcggggcga gcgcaagcgc agcaagctgg
     8161 acgtgacgta tgactacggc gacggcaagc ggatcgagtt cagcggcccg gagccgctgg
     8221 gcgctgatga tctgcgcatc ctgcaagggc tggtggccat ggctgggcct aatggcctag
     8281 tgcttggccc ggaacccaag accgaaggcg gacggcagct ccggctgttc ctggaaccca
     8341 agtgggaggc cgtcaccgct gaatgccatg tggtcaaagg tagctatcgg gcgctggcaa
     8401 aggaaatcgg ggcagaggtc gatagtggtg gggcgctcaa gcacatacag gactgcatcg
     8461 agcgcctttg gaaggtatcc atcatcgccc agaatggccg caagcggcag gggtttcggc
     8521 tgctgtcgga gtacgccagc gacgaggcgg acgggcgcct gtacgtggcc ctgaacccct
     8581 tgatcgcgca ggccgtcatg ggtggcggcc agcatgtgcg catcagcatg gacgaggtgc
     8641 gggcgctgga cagcgaaacc gcccgcctgc tgcaccagcg gctgtgtggc tggatcgacc
     8701 ccggcaaaac cggcaaggct tccatagata ccttgtgcgg ctatgtctgg ccgtcagagg
     8761 ccagtggttc gaccatgcgc aagcgccgca agcgggtgcg cgaggcgttg ccggagctgg
     8821 tcgcgctggg ctggacggta accgagttcg cggcgggcaa gtacgacatc acccggccca
     8881 aggcggcagg ctgacccccc ccactctatt gtaaacaaga catttttatc ttttatattc
     8941 aatggcttat tttcctgcta attggtaata ccatgaaaaa taccatgctc agaaaaggct
     9001 taacaatatt ttgaaaaatt gcctactgag cgctgccgca cagctccata ggccgctttc
     9061 ctggctttgc ttccagatgt atgctcttct gctcccgaac gccagcaaga cgtagcccag
     9121 cgcgtcggcc agcttgcaat tcgcgctaac ttacattaat tgcgttgcgc tcactgcccg
     9181 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     9241 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac
     9301 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgtggttt
     9361 gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt
     9421 cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc ccggactcgg
     9481 taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa
     9541 cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc
     9601 cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca
     9661 gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac
     9721 ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac
     9781 tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag
     9841 cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc
     9901 gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca
     9961 tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt
    10021 gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc
    10081 ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca
    10141 ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct
    10201 gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca
    10261 ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcaccatt
    10321 cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg aattgcaagc tgatccgggc
    10381 ttatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga agctgtggta
    10441 tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc tcaaggcgca ctcccgttct
    10501 ggataatgtt ttttgcgccg acatcataac ggttctggca aatattctga aatgagctgt
    10561 tgacaattaa tcatcggctc gtataatgtg tggaattgtg agcggataac aatttcacac
    10621 aggaattata aaggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
    10681 taaagggaac aaaagctggg taccgggccc cccctcgagg tcgacggtat cgataagctt
    10741 gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc caccgcggtg
    10801 gagctccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
    10861 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
    10921 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
    10981 cagcctgaat ggcgaatgga aattgtaagt gcacaaagtg cggtcgcgga tgagagaaga
    11041 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatt
//
