LOCUS       40924_25856        4753 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAH01, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Leeds JA, Beckwith J.
  TITLE     Lambda repressor N-terminal DNA-binding domain as an assay for 
            protein transmembrane segment interactions in vivo
  JOURNAL   J. Mol. Biol. 280 (5), 799-810 (1998)
  PUBMED    9671551
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Leeds JA, Beckwith J.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-FEB-1999) Microbiology, Harvard Medical School, 200 
            Longwood Avenue Bldg. D-1, Boston, MA 02115, USA
REFERENCE   3  (bases 1 to 4753)
  AUTHORS   Leeds JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-1999) Microbiology, Harvard Medical School, 200 
            Longwood Avenue Bldg. D-1, Boston, MA 02115, USA
REFERENCE   4  (bases 1 to 4753)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4753)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Mol. 
            Biol."; date: "1998"; volume: "280"; issue: "5"; pages: "799-810"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-FEB-1999) Microbiology, Harvard Medical School, 200 Longwood 
            Avenue Bldg. D-1, Boston, MA 02115, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (20-OCT-1999) Microbiology, Harvard Medical School, 200 Longwood 
            Avenue Bldg. D-1, Boston, MA 02115, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On Oct 20, 1999 this sequence version replaced AF129432.1.
FEATURES             Location/Qualifiers
     source          1..4753
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        29..59
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    67..83
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             104..508
                     /codon_start=1
                     /gene="cI"
                     /product="lambda cI repressor"
                     /label=cI
                     /note="transcriptional repressor"
                     /protein_id="AAD31808.1"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTC"
     gene            104..508
                     /gene="cI"
                     /label=cI
     misc_feature    104..379
                     /gene="cI"
                     /label=Region: DNA-binding domain
                     /note="Region: DNA-binding domain"
     misc_feature    380..505
                     /gene="cI"
                     /label=Region: linker region
                     /note="Region: linker region"
     misc_feature    500..505
                     /gene="cI"
                     /label=AflIII restriction site
                     /note="AflIII restriction site"
     promoter        complement(728..830)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(1356..1901)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     promoter        2013..2041
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             2089..3276
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
ORIGIN
        1 gaattctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt
       61 gtggaattgt gagcggataa caatttcaca caggaaacag catatgagca caaaaaagaa
      121 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa
      181 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc
      241 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct
      301 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta
      361 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt
      421 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga
      481 tgcggagaga tgggtaagca catgttaaga attccggatg agcattcatc aggcgggcaa
      541 gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg
      601 ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct
      661 caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt
      721 tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta
      781 gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgccgatca acgtctcatt
      841 ttcgccaaaa gttggcccag ggcttcccgg tatcaacagg gacaccagga tttatttatt
      901 ctgcgaagtg atcttccgtc acaggtattt attcggcgca aagtgcgtcg ggtgatgctg
      961 ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc ttctgtttct
     1021 atcagctgtc cctcctgttc agctactgac ggggtggtgc gtaacggcaa aagcaccgcc
     1081 ggacatcagc gctagcggag tgtatactgg cttactatgt tggcactgat gagggtgtca
     1141 gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg
     1201 atacaggata tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1261 gcgagcggaa atggcttacg aacggggcgg agatttcctg gaagatgcca ggaagatact
     1321 taacagggaa gtgagagggc cgcggcaaag ccgtttttcc ataggctccg cccccctgac
     1381 aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa acccgacagg actataaaga
     1441 taccaggcgt ttccccctgg cggctccctc gtgcgctctc ctgttcctgc ctttcggttt
     1501 accggtgtca ttccgctgtt atggccgcgt ttgtctcatt ccacgcctga cactcagttc
     1561 cgggtaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc agtccgaccg
     1621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gaaagacatg caaaagcacc
     1681 actggcagca gccactggta attgatttag aggagttagt cttgaagtca tgcgccggtt
     1741 aaggctaaac tgaaaggaca agttttggtg actgcgctcc tccaagccag ttacctcggt
     1801 tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc ctgcaaggcg gttttttcgt
     1861 tttcagagca agagattacg cgcagaccaa aacgatctca agaagatcat cttattaatc
     1921 agataaaata tttctagatt tcagtgcaat ttatctcttc aaatgtagca cctgaagtca
     1981 gccccatacg atataagttg taattctcat gtttgacagc ttatcatcga taagctttaa
     2041 tgcggtagtt tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac
     2101 aatgcgctca tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt
     2161 atgccggtac tgccgggcct cttgcgggat atcgtccatt ccgacagcat cgccagtcac
     2221 tatggcgtgc tgctagcgct atatgcgttg atgcaatttc tatgcgcacc cgttctcgga
     2281 gcactgtccg accgctttgg ccgccgccca gtcctgctcg cttcgctact tggagccact
     2341 atcgactacg cgatcatggc gaccacaccc gtcctgtgga tcctctacgc cggacgcatc
     2401 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     2461 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     2521 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     2581 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     2641 cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg
     2701 tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc
     2761 gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc
     2821 gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc
     2881 ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg
     2941 gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc
     3001 cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg
     3061 tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc
     3121 agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc
     3181 acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg
     3241 ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg
     3301 ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg
     3361 cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc
     3421 ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt
     3481 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     3541 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     3601 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcccct acgtgctgct
     3661 gaagttgccc gcaacagaga gtggaaccaa ccggtgatac cacgatacta tgactgagag
     3721 tcaacgccat gagcggcctc atttcttatt ctgagttaca acagtccgca ccgctgtccg
     3781 gtagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     3841 ttatcatgca actcgtagga caggtgccgg cagcgcccaa cagtcccccg gccacggggc
     3901 ctgccaccat acccacgccg aaacaagcgc cctgcaccat tatgttccgg atctgcatcg
     3961 caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctaaccgt
     4021 ttttatcagg ctctgggagg cagaataaat gatcatatcg tcaattatta cctccacggg
     4081 gagagcctga gcaaactggc ctcaggcatt tgagaagcac acggtcacac tgcttccggt
     4141 agtcaataaa ccggtaaacc agcaatagac ataagcggct atttaacgac cctgccctga
     4201 accgacgacc gggtcgaatt tgctttcgaa tttctgccat tcatccgctt attatcactt
     4261 attcaggcgt agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc
     4321 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     4381 catcacagac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     4441 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     4501 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     4561 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     4621 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     4681 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     4741 tcattgccat acg
//
