LOCUS       40924_25836        7616 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAC91, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7616)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 7616)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 7616)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7616)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..501
                     /label=HIS3 promoter
     misc_feature    502..1915
                     /label=CG HIS3 w promoter and terminator
                     /note="CG HIS3 w promoter and terminator"
     regulatory      1928..2189
                     /label=PCyc1 promoter
                     /note="PCyc1 promoter"
                     /regulatory_class="promoter"
     CDS             2196..2537
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      3105..3799
                     /label=C. albicans TadH1 terminator
                     /note="C. albicans TadH1 terminator"
                     /regulatory_class="terminator"
     misc_feature    3800..4118
                     /label=HIS3 3UTR
                     /note="HIS3 3UTR"
     promoter        complement(4164..4182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4189..4205)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4346..4801
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5439..5543
                     /label=AmpR promoter
     CDS             5544..6401
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6575..7163
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7451..7472
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7487..7517
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7525..7541
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7549..7565
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7586..7604
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 cgggcccgag cagatccgcc aggcgtgtat atatagcgtg gatggccagg caactttagt
     1981 gctgacacat acaggcatat atatatgtgt gcgacgacac atgatcatat ggcatgcatg
     2041 tgctctgtat gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata
     2101 cattaggacc tttgcagcat aaattactat acttctatag acacacaaac acaaatacac
     2161 acactaaatt aataatgact gaactcgagg gagctgccaa ttttaatcaa agtgggaata
     2221 ttgctgatag ctcattgtcc ttcactttca ctaacagtag caacggtccg aacctcataa
     2281 caactcaaac aaattctcaa gcgctttcac aaccaattgc ctcctctaac gttcatgata
     2341 acttcatgaa taatgaaatc acggctagta aaattgatga tggtaataat tcaaaaccac
     2401 tgtcacctgg ttggacggac caaactgcgt ataacgcgtt tggaatcact acagggatgt
     2461 ttaataccac tacaatggat gatgtatata actatctatt cgatgatgaa gataccccac
     2521 caaacccaaa aaaagagggt tctggttctg gttctggttc taaaaaagcc acatgcttaa
     2581 ctgacgatca acgctggcaa tctgtcttag cccgcgaccc gaatgccgac ggcgaattcg
     2641 ttttcgccgt gcgtaccaca ggcatctttt gccgtccgtc ttgccgcgcc agacatgctt
     2701 tgcgggaaaa cgtctccttc tacgcaaatg ccagcgaggc actcgccgct ggctttcgcc
     2761 cctgcaaacg ttgtcagcca gaaaaagcca atgcccagca acatcggttg gataaaatca
     2821 cccacgcgtg tcgactgctg gaacaggaaa cgcctgtaac gctggaagcc ttagccgacc
     2881 aggtggcgat gagtccattt catctacatc ggttgtttaa agcgactacc ggaatgacgc
     2941 ctaaagcctg gcaacaggcc tggcgcgctc gccgtttgcg cgaatcgctg gcgaaagggg
     3001 agagcgtgac gacgtctatt cttaacgccg gattccccga cagcagcagt tactatcgca
     3061 aagctgacga aacgctgggc atgacggcta aacaattccg ttaataagca aatagctaaa
     3121 ttatatacga attaatatta tgattaagtg tttacgtgag tgcgatattt ttattactat
     3181 cttatacagt tgtatatact ctataaaatg agttgtctat taattaacgc gatgaatgct
     3241 ttctgggttt acctctccaa caactctagt ttacttctca atacattcaa ttgtatttga
     3301 tttgtcaata cttcatcatt aatcaattct atagttttgt ttttctcgtt tatttccaaa
     3361 tttaatgcat caattttatt attcaatttg tcgttgattt tggttaatga ttttatggtt
     3421 tgatctctgg cattgattgt ttgtgttagt ttttcattat tgataattaa attatttaag
     3481 ttagttatca actcggtgtt ttcaagtttc aagttttcaa tttctttaga gtttattaga
     3541 tttgtcaaag tttctgaatt gcttgattgg tcctgtagaa gagtatttgt tgttgtggat
     3601 aattgattca atttttgaga caattgctgg aaggcgttga aatatctagc atcaatctca
     3661 tggttttttt cccgagagtc tcgtagattc aattgtttta atatatcttg ggaccactct
     3721 tgatttgaac tcatggaaat taaactgggt gttgtgttgt ggtgtaatga ttgtaccccc
     3781 tttgcttata attgtgtggg agatggagga acgggaaaaa gttagttgtg gtgataggtg
     3841 gcaagtggta ttccgtaaga acaacaagaa aagcatttca tattatggct gaactgagcg
     3901 aacaagtgca aaatttaagc atcaacgaca acaacgagaa tggttatgtt cctcctcact
     3961 taagaggaaa accaagaagt gccagaaata acagtagcaa ctacaataac aacaacggcg
     4021 gctacaacgg tggccgtggc ggtggcagct tctttagcaa caaccgtcgt ggtggttacg
     4081 gcaacggtgg tttcttcggt ggaaacaacg gtggcagcgg ccggccattt aaatgtttaa
     4141 acggccacgc tggcccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt
     4201 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     4261 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4321 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4381 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4441 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4501 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4561 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4621 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4681 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4741 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4801 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagggtaat
     4861 aactgatata attaaattga agctctaatt tgtgagttta gtattaggat ctctaccctt
     4921 ggcgaaaagt cctctgccaa caatgatgat atctgatcca ccacttacaa cttcgtcgac
     4981 ggttctgtac tgctgaccca atatgcgtat atataccaat ctaagtctgt gctccttcct
     5041 tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa accgaaatca
     5101 aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca
     5161 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5221 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5281 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5341 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcggcg
     5401 cgccgaggac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5461 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5521 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5581 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5641 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5701 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5761 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5821 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5881 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5941 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6001 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6061 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6121 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6181 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6241 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6301 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6361 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6421 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6481 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6541 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6601 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6661 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6721 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6781 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6841 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6901 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6961 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7021 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7081 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7141 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7201 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7261 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7321 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7381 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     7441 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7501 ttccggctcc tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7561 atgaccatga ttacgccaag ctcggaatta accctcacta aagggaacaa aagctg
//
