LOCUS       40924_25831        8851 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pJAC90, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim W, Voigt CA.
  TITLE     Genetic sensor for strong methylating compounds
  JOURNAL   ACS Synth Biol 2 (10), 614-624 (2013)
  PUBMED    24032656
REFERENCE   2  (bases 1 to 8851)
  AUTHORS   Moser F, Horwitz A, Chen J, Lim WA, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-JUL-2013) Biological Engineering, MIT, 500 Tech 
            Square, Rm 209D, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 8851)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8851)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol"; date: "2013"; volume: "2"; issue: "10"; pages: "614-624"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (05-JUL-2013) Biological Engineering, MIT, 500 Tech Square, Rm 209D,
            Cambridge, MA 02139, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        314..501
                     /label=HIS3 promoter
     misc_feature    502..1915
                     /label=CG HIS3 w promoter and terminator
                     /note="CG HIS3 w promoter and terminator"
     promoter        2711..3415
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             3431..3772
                     /label=GAL4 activation domain
                     /note="activation domain of the GAL4 transcriptional
                     activator"
     regulatory      4340..5034
                     /label=C. albicans TadH1 terminator
                     /note="C. albicans TadH1 terminator"
                     /regulatory_class="terminator"
     misc_feature    5035..5353
                     /label=HIS3 3UTR
                     /note="HIS3 3UTR"
     promoter        complement(5399..5417)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5424..5440)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      5581..6036
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6674..6778
                     /label=AmpR promoter
     CDS             6779..7636
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7810..8398
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8686..8707
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8722..8752
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8760..8776
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8784..8800
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8821..8839
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggccggccat ttaaatgttt aaacggccac gctggccgct gcacggtcct gttccctagc
       61 atgtacgtga gcgtatttcc ttttaaacca cgacgctttg tcttcattca acgtttccca
      121 ttgttttttt ctactattgc tttgctgtgg gaaaaactta tcgaaagatg acgacttttt
      181 cttaattctc gttttaagag cttggtgagc gctaggagtc actgccaggt atcgtttgaa
      241 cacggcatta gtcagggaag tcataacaca gtcctttccc gcaattttct ttttctatta
      301 ctcttggcct cctctagtac actctatatt tttttatgcc tcggtaatga ttttcatttt
      361 tttttttcca cctagcggat gactcttttt ttttcttagc gattggcatt atcacataat
      421 gaattataca ttatataaag taatgtgatt tcttcgaaga atatactaaa aaatgagcag
      481 gcaagataaa cgaaggcaaa gaggctctat atgtatataa agctgcggat gccttctgat
      541 ttaatctagt tctagtttac ttcaggttta cttaaagttg agataattga agcaaagaga
      601 agcatcgagc aaaggatggc gtttgttaag agggttacgc aggagacgaa tatacagctg
      661 gcgctggatc ttgacggtgg gtctgtttct gtacgggaga gcatactggg caaggaatat
      721 gctagtggtg atgggcagac catccatgtg cacactggag ttgggttttt ggaccacatg
      781 ttgactgcgc tggcgaagca tggcgggtgg tctctgatcc tggagtgtat aggggacttg
      841 cacatcgatg accaccacac tgttgaggac tgtgggattg cgctgggcca agcgttcaag
      901 gaggcgcttg gctccgtgcg tggtatcaag aggttcgggc atgggtttgc accactggac
      961 gaggcgctga gccgcgctgt ggttgacttc tccaataggc ctttcgccgt ggtggagctg
     1021 ggcctgaagc gagaacgcat aggccagcta tccacagaga tgatcccgca cttcttggag
     1081 agtttcgcca ctgaggcgcg tatcaccatg catgtggact gtctgcgggg caccaacgac
     1141 caccaccgct ccgaatcagc tttcaaggcg ctcgccatcg ccatcagaga ggcaagaaca
     1201 cctacgggtc gcgatgacgt tccatccact aagggtgtcc tagcatagaa cacagcccac
     1261 agctaccacc atcaacaata tttatataga taacgtacac atagaaatca cacaaacaga
     1321 gtatttattc ttaactacat gaactaccat cagaccgtct gggccactat ataatgtgct
     1381 attcataaac gtgatcactc tacgtagcag gcgaccccag gtgaaaattt ttcagcgagc
     1441 tgccagattg tcaggtgaaa aactgaaaaa aacttctggg cgatgagctt gtggcgggaa
     1501 attaagtata taaagcagtt agtttcttct gcttcttgtg gttctgggta ttcttgtaat
     1561 atttggaata atagaaaacc aagactactg cctttctttt catattatag aggaagaaat
     1621 acgcacgaac acgatataga ggtaaatttt ttgcaaaaag ataccaaact ataaaagaaa
     1681 tagaagacta aactacaaaa aataaagaaa gtaatacata tatccaatat gactgagtta
     1741 gcagagcaag tttccatgcc tgaagggcaa caaagacgtg gtggttacgt tccacctcac
     1801 atccgtaacg gtggtggcag aagaggcgat tctgctcctc gtggtggttt caacaacggt
     1861 ggcggccgtg gtggtttctt cagaaacaac ggtgaccgtg gtggattcgg ctttgggtac
     1921 cgggccctaa aacaagaaga gggttgacta catcacgatg agggggatcg aagaaatgat
     1981 ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa gggtcgaacg
     2041 aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg aaaaaacgag
     2101 tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg ccggcccggc
     2161 gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt
     2221 ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg gggttgcgat
     2281 gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct gagtgcattt
     2341 gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt tgccggtggt
     2401 gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct agagtacttt
     2461 gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat acaacactgg
     2521 aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt attatgttac
     2581 aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag tccaatgcta
     2641 gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta aaccgtggaa
     2701 tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc ttttctggca
     2761 accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa catgtaggtg
     2821 gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc taaacaagac
     2881 tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact gtagccctag
     2941 acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc catctattga
     3001 agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct cccccgttgt
     3061 tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga aaaaattaac
     3121 gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta tctcgaagca
     3181 cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca acggtatacg
     3241 gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt gctatcaagt
     3301 ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg ttccctttct
     3361 tccttgtttc tttttctgca caatatttca agctatacca agcatacaat caactatctc
     3421 atatacaatg gccaatttta atcaaagtgg gaatattgct gatagctcat tgtccttcac
     3481 tttcactaac agtagcaacg gtccgaacct cataacaact caaacaaatt ctcaagcgct
     3541 ttcacaacca attgcctcct ctaacgttca tgataacttc atgaataatg aaatcacggc
     3601 tagtaaaatt gatgatggta ataattcaaa accactgtca cctggttgga cggaccaaac
     3661 tgcgtataac gcgtttggaa tcactacagg gatgtttaat accactacaa tggatgatgt
     3721 atataactat ctattcgatg atgaagatac cccaccaaac ccaaaaaaag agggttctgg
     3781 ttctggttct ggttctaaaa aagccacatg cttaactgac gatcaacgct ggcaatctgt
     3841 cttagcccgc gacccgaatg ccgacggcga attcgttttc gccgtgcgta ccacaggcat
     3901 cttttgccgt ccgtcttgcc gcgccagaca tgctttgcgg gaaaacgtct ccttctacgc
     3961 aaatgccagc gaggcactcg ccgctggctt tcgcccctgc aaacgttgtc agccagaaaa
     4021 agccaatgcc cagcaacatc ggttggataa aatcacccac gcgtgtcgac tgctggaaca
     4081 ggaaacgcct gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc catttcatct
     4141 acatcggttg tttaaagcga ctaccggaat gacgcctaaa gcctggcaac aggcctggcg
     4201 cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc gtgacgacgt ctattcttaa
     4261 cgccggattc cccgacagca gcagttacta tcgcaaagct gacgaaacgc tgggcatgac
     4321 ggctaaacaa ttccgttaat aagcaaatag ctaaattata tacgaattaa tattatgatt
     4381 aagtgtttac gtgagtgcga tatttttatt actatcttat acagttgtat atactctata
     4441 aaatgagttg tctattaatt aacgcgatga atgctttctg ggtttacctc tccaacaact
     4501 ctagtttact tctcaataca ttcaattgta tttgatttgt caatacttca tcattaatca
     4561 attctatagt tttgtttttc tcgtttattt ccaaatttaa tgcatcaatt ttattattca
     4621 atttgtcgtt gattttggtt aatgatttta tggtttgatc tctggcattg attgtttgtg
     4681 ttagtttttc attattgata attaaattat ttaagttagt tatcaactcg gtgttttcaa
     4741 gtttcaagtt ttcaatttct ttagagttta ttagatttgt caaagtttct gaattgcttg
     4801 attggtcctg tagaagagta tttgttgttg tggataattg attcaatttt tgagacaatt
     4861 gctggaaggc gttgaaatat ctagcatcaa tctcatggtt tttttcccga gagtctcgta
     4921 gattcaattg ttttaatata tcttgggacc actcttgatt tgaactcatg gaaattaaac
     4981 tgggtgttgt gttgtggtgt aatgattgta ccccctttgc ttataattgt gtgggagatg
     5041 gaggaacggg aaaaagttag ttgtggtgat aggtggcaag tggtattccg taagaacaac
     5101 aagaaaagca tttcatatta tggctgaact gagcgaacaa gtgcaaaatt taagcatcaa
     5161 cgacaacaac gagaatggtt atgttcctcc tcacttaaga ggaaaaccaa gaagtgccag
     5221 aaataacagt agcaactaca ataacaacaa cggcggctac aacggtggcc gtggcggtgg
     5281 cagcttcttt agcaacaacc gtcgtggtgg ttacggcaac ggtggtttct tcggtggaaa
     5341 caacggtggc agcggccggc catttaaatg tttaaacggc cacgctggcc caattcgccc
     5401 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     5461 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     5521 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     5581 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5641 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5701 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5761 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5821 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5881 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     5941 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6001 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
     6061 gcatctgtgc ggtatttcac accgcatagg gtaataactg atataattaa attgaagctc
     6121 taatttgtga gtttagtatt aggatctcta cccttggcga aaagtcctct gccaacaatg
     6181 atgatatctg atccaccact tacaacttcg tcgacggttc tgtactgctg acccaatatg
     6241 cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg ttcggagatt
     6301 accgaatcaa aaaaatttca aggaaaccga aatcaaaaaa aagaataaaa aaaaaatgat
     6361 gaattgaaaa ggtggtatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     6421 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6481 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     6541 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     6601 atgtcatgat aataatggtt tcttagacgt cggcgcgccg aggacgtcag gtggcacttt
     6661 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6721 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6781 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6841 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6901 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6961 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     7021 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     7081 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     7141 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     7201 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     7261 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     7321 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     7381 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7441 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7501 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7561 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7621 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7681 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7741 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7801 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7861 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7921 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7981 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     8041 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     8101 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     8161 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     8221 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     8281 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     8341 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     8401 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8461 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     8521 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     8581 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     8641 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     8701 cactcattag gcaccccagg ctttacactt tatgcttccg gctcctatgt tgtgtggaat
     8761 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcgg
     8821 aattaaccct cactaaaggg aacaaaagct g
//
