LOCUS       V005386                 8317 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005386
VERSION     V005386
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8317)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8317)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8317)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8317)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1..135)
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(560..580)
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(710..775)
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(777..851)
                     /codon_start=1
                     /note="unnamed protein product; part of SV40 small
                     t-antigen"
                     /protein_id="SJL87422.1"
                     /translation="DLCEGTLLLWCDIIGQTTYRDLKL"
     misc_feature    complement(985..2995)
                     /label="3' UTR of hCDH1"
                     /note="3' UTR of hCDH1"
     CDS             complement(2999..5644)
                     /gene="CDH1"
                     /label="Cadherin-1"
                     /note="Cadherin-1 from Homo sapiens. Accession#: P12830"
     misc_feature    complement(5645..5738)
                     /label="5' UTR of hCDH1"
                     /note="5' UTR of hCDH1"
     promoter        complement(5757..6086)
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     misc_feature    6237..6377
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6563..7151)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7325..8182)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8183..8287)
                     /label="AmpR promoter"
ORIGIN
        1 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
       61 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
      121 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
      181 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
      241 ctctagtcaa ggcactatac atcaaatatt ccttattaac ccctttacaa attaaaaagc
      301 taaaggtaca caatttttga gcatagttat taatagcaga cactctatgc ctgtgtggag
      361 taagaaaaaa cagtatgtta tgattataac tgttatgcct acttataaag gttacagaat
      421 atttttccat aattttcttg tatagcagtg cagctttttc ctttgtggtg taaatagcaa
      481 agcaagcaag agttctatta ctaaacacag catgactcaa aaaacttagc aattctgaag
      541 gaaagtcctt ggggtcttct acctttctct tcttttttgg aggagtagaa tgttgagagt
      601 cagcagtagc ctcatcatca ctagatggca tttcttctga gcaaaacagg ttttcctcat
      661 taaaggcatt ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac
      721 aaacaattag aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag
      781 agctttaaat ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct
      841 tcacaaagat ctggtaccat cgatgaattc gagctcgccc ggggatcctc tagatttttt
      901 tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt ttaccggttt
      961 aacaaacttg cttaataaaa ttcntaaaaa tgcatctttg agaatacttt tctcagcttg
     1021 aattgttttc cttttccacc cccaaagaaa atacacaatt atcagcaccc acacatgtat
     1081 acactcaaaa ctacagtgac attctctaca cagaactata ttcgatatag cttgaactgc
     1141 cgaaaaatca agacaattcc aaaaagtgat tgcagggttg atttttttct ccaaaacact
     1201 tgagaaacag taaagctatt tcaacaaaag tcttttcttt gattgtcaaa agttgaaatt
     1261 cacatttaaa taaaaagaga tccaaatcaa gatcctcact accccctacc cctcaactaa
     1321 ccccctttag ggccacattt tcttcttgct cctaagaaaa aatttggaat tttgaatatt
     1381 ctcggttttc tgtgcacacc tggaattggg caaatgtgtt cagctcagcc agcattttct
     1441 gtagacatca tcaaaagcag gcacttgggg attctgggct ttgagtacaa accacggatc
     1501 ttgtgtcaga aacacatgtt gagactcctc cattccttcc agaattttca gagatgaggt
     1561 agacccacct caatcatcct cagcatcagt ttgctaaatt gccaggctca atgacaagct
     1621 ctcctgccat ctccaagccc acttttcata gttccgctct gtctttggct gcagcacttt
     1681 aggcactatt ctaagtcctg gagtatatca ctcttgcttc agagctaaat aaacattaat
     1741 gaacacactt actcagaaca agtcactgga tagctgccca ttgcaagtta catactcagg
     1801 agatgaaaga gggaagccat taaaggtctt cagagtagac aatacctagt caagatgtgg
     1861 ccagacaaag acacaaactt cttaccctaa aagagcccaa taatttctgc atcagagaac
     1921 tcctatcttg ggcaaagcaa ctgaattcag gagtgagagt tgatattaaa acatggaggc
     1981 tgggcacagc agctcatgcc cacaatccca gcactttggg gggccaaggc agaaggattg
     2041 cttgaggcca ggagtttaag accagcttgg gcaacatagc aagaccccat ctgtacaaaa
     2101 aaataaaaag gaggctggtg ggagaactgc ttgagcccca gagtttgagg ttacagtgag
     2161 ctatgatcac atcactgcat cccaggcctg ggcgatggag cgaaactgtc tcttaaaaaa
     2221 tggcagggag ttggggagct gggcaggtgc agtggctcat gtctgtaatc ccaatactct
     2281 gggaggccaa gatgggagga tcacttgagc ccaggagttt gagaccagcc tgggtaacac
     2341 agggagaccc cgtctcaaat atttaaaaaa ttagtcatgc gtagtggtgc atgcctgtgg
     2401 tcccagctac ttgggaggct gaggtgcaag gatagcttga gcctgggagg acaaggctgc
     2461 agtgagctgt gattgcacca ctgcactcca gcctggccga tagaatgaga ccctgtctta
     2521 aaaaaaaaaa aaaaggcaga gggacacacc agtgtagtaa tgagcagaag cacacaaatt
     2581 ttttttaaaa aattatatac acaaaacaac agaactagag tagctgactt ctccccttct
     2641 ttttaaaagt gagacaaaat tccttaagaa aatctgcaag gtgctgggtg aaccttctga
     2701 tgctaaagtt gttcttctgg aatattaaaa tttgggtatc ttttggacat caccaccatg
     2761 taaagagtga tgggaaaaaa aaaaaagctt taagaaataa gtcgaaactt ttctaacaca
     2821 aacttattag agctataaag tggagagagt ataaagctat actaactgca tcactaacca
     2881 gtctcttttt tttccccaga aactcatctc aagggaaggg agctgaaaaa ccaccagcaa
     2941 cgtgatttct gcatttccca gcacatgggt ctggggcccg cctctctcga gtcccctagt
     3001 cgtcctcgcc gcctccgtac atgtcagcca gcttcttgaa gcgattgccc cattcgttca
     3061 agtagtcata gtcctggtct ttgtctgact ctgaggagtt cagggagctc agactagcag
     3121 cttcggaacc gcttccttca tagtcaaaca cgagcagaga atcataaggc ggggctgtgg
     3181 ggtcagtatc agccgctttc agattttcat caataaaatt tccaatttca tcgggattgg
     3241 cagggcgggg aagataccgg gggacactca tgagggttgg tgcaacgtcg ttacgagtca
     3301 cttcaggccg agcgtccagg cccctgtgca gctggctcaa gtcaaagtcc tggtcctctt
     3361 ctccgcctcc ttcttcatca tagtaataaa cgttgtcccg ggtgtcatcc tctgggggca
     3421 gtaagggctc tttgaccacc gctctcctcc gaagaaacag caagagcagc agaatcagaa
     3481 ttagcaaagc aagaattcct ccaagaatcc ccagaatggc aggaatttgc aatcctgctt
     3541 cgacaggctg tgccttccta cagacgccgg cggccccttc acagtcacac acgctgacct
     3601 ctaaggtggt cacttggtct ttattctggt tatccatgag cttgagattg attttgtagt
     3661 cacccacctc taaggccatc tttggcttca aaatgataga ttcttgggtt gggtcgttgt
     3721 actgaatggt ccagttggca ctcgccccgt gtgttagttc tgctgtgaag ggagatgtat
     3781 tgggaggaag gtctgcatca atgatgttta tgacctgagg ctttggattc ctctcacaga
     3841 agaatatagt tcgaggttct ggtatggggg cgttgtcatt cacatcagac aggatcagca
     3901 gaagtgtccc tgttccagta gcaactggag aaccattgtc tgtagctatg attagggctg
     3961 tgtacgtgct gttcttcacg tgctcaaaat cctccctgtc cagctcagcc cgagtggaaa
     4021 tggcaccagt gtccggatta atctccagcc agttggcagt gtctctccaa atccgatatg
     4081 ttattttctg ttccataaat gtgtctggct cctgggcagt gtaggatgtg atttcctggc
     4141 ccacgccaaa gtcctcggac acttccactc tcttttcagg aggcacaaag atgggggctt
     4201 cattcacatc cagcacatcc acggtgacgg tggctgtgga ggtggtgaga gagacctcaa
     4261 aaggtaccac attcgtcact gctacgtgta gaatgtactg ctgcttggcc tcaaaatcca
     4321 agccctttgc tgttttcaaa atgccatcgt tgttcactgg atttgtggtg acgacaaatt
     4381 gtccaccatc atcattcaat atggtgtata cagcctccca cgctggggta ttgggggcat
     4441 cagcatcagt cactttcagt gtggtgatta cgacgttagc ctcgttctca ggcacctgac
     4501 ccttgtacgt ggtgggattg aagatcggag gattatcgtt ggtgtcagtg actgtgatca
     4561 cagctgttgc tgttgtgctt aacccctcac cttgaaggtc agcagcttga accaccaggg
     4621 tatacgtagg gaaactctct cggtccagcc cagtggtgac cacactgatg actcctgtgt
     4681 tcctgttaat ggtgaacata tttttgtcag ggagctcagg atcttggctg aggatggtgt
     4741 aagcgatggc ggcattgtag gtgttcacat catcgtccgc gtctgtggct gtgacctcca
     4801 tcacagaggt tcctggaaga gcaccttcca tgacagaccc cttaaagacc tcctgggtga
     4861 attcgggctt gttgtcattc tgatcggtta ccgtgatcaa aatctccatt ggatcctcaa
     4921 ctgcattccc gttggatgac acagcgtgag agaagagagt gtatgtggca atgcgttctc
     4981 tatccagagg ctctgtcacc ttcagccatc ctgtttctct ttcaataata aagacaccaa
     5041 cagggggtgt gtcagctcct tggccagtga tgctgtagaa aaccttgcct tctttgtctt
     5101 tgttggattt gatctgaacc aggtttttag gaaatgggcc tttttcattt tctgggcagc
     5161 tgatgggagg aataacccag tctctcttct gtcttctgag gccaggagag gagttgggaa
     5221 atgtgagcaa ttctgcttgg attccagaaa cggaggcctg atggggcggg gggcggtggt
     5281 ggtgccccac tgtattcagc gtgactttgg tggaaaactt tctgtaggtg gagtcccagg
     5341 cgtagaccaa gaaatggatc tgtgggttat gaaaccgtag aggccttttg actgtaatca
     5401 caccatctgt gcccactttg aatcgggtgt cgagggaaaa ataggctgtc ctttgtcgac
     5461 cggtgcaatc ttcaaaattc actctgccca ggacgcggcc tctctccagg tggcgccggg
     5521 gcaccgtgaa cgtgtagctc tcggcgtcaa agccagggtg gcagggctcc ggctcctggc
     5581 agagccaaga ggagacctgc agcagcagca gcagcgccga gaggctgcgg ctccaagggc
     5641 ccatggctgg ccggggacgc cgagcgaggg caggcgccgg gtgcggtcgg gtcgggccgg
     5701 gctggagcgg gctggagtct gaactgactt ccgcaagcac ctccggaatt cagctttttg
     5761 caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag
     5821 gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg
     5881 gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac
     5941 taattgagat gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac
     6001 ctggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     6061 gactttccac accctaactg acacacattc cacagctgcc tcgcgcgttt cggtgatgac
     6121 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     6181 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     6241 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag
     6301 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
     6361 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6421 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6481 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     6541 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     6601 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     6661 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     6721 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     6781 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     6841 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6901 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6961 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     7021 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7081 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7141 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7201 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7261 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7321 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7381 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7441 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7501 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     7561 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     7621 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     7681 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     7741 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     7801 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     7861 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7921 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7981 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8041 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8101 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8161 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8221 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8281 ttccgcgcac atttccccga aaagtgccac ctgacgt
//
