LOCUS       62056_13070        2636 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     terminator      270..356
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      448..475
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        730..834
                     /label=AmpR promoter
     CDS             835..1692
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1866..2454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctgaatt catggctagc gttactgctt ggtaccgagc tcacctcgag
      241 ggatcctgat aattaatttt tccaggcatc aaataaaacg aaagtctcag tcgaaagact
      301 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
      361 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc
      421 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt
      481 ttctacaaac tctgccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
      541 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
      601 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
      661 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
      721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     1021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     1081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2521 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2581 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaaga
//
