LOCUS       V014241                 5490 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014241
VERSION     V014241
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5490)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5490
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             219..935
                     /label="EGFP"
                     /note="enhanced GFP"
     RBS             1008..1028
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1042..1878
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        1898..1926
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(3094..3110)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3584..3688
                     /label="AmpR promoter"
     CDS             3689..4546
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4720..5308
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgattacg ccaagcttat ggtgagcaag ggcgaggagc
      241 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      301 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      361 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      421 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      481 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      541 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      601 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      661 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      721 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      781 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      841 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      901 ccgggatcac tctcggcatg gacgagctgt acaagtagct gatagacttc gaaatttgac
      961 agctagctca gtcctaggta taatactagt tttcacacag gaaacagttg tttaacttta
     1021 agaaggagtt acgccaagct tatggtgacg gtgttcggca ttctgaatct caccgaggac
     1081 tccttcttcg atgagagccg gcggctagac cccgccggcg ctgtcaccgc ggcgatcgaa
     1141 atgctgcgag tcggatcaga cgtcgtggat gtcggaccgg ccgccagcca tccggacgcg
     1201 aggcctgtat cgccggccga tgagatcaga cgtattgcgc cgctcttaga cgccctgtcc
     1261 gatcagatgc accgtgtttc aatcgacagc ttccaaccgg aaacccagcg ctatgcgctc
     1321 aagcgcggcg tgggctacct gaacgatatc caaggatttc ctgaccctgc gctctatccc
     1381 gatattgctg aggcggactg caggctggtg gttatgcact cagcgcagcg ggatggcatc
     1441 gccacccgca ccggtcacct tcgacccgaa gacgcgctcg acgagattgt gcggttcttc
     1501 gaggcgcggg tttccgcctt gcgacggagc ggggtcgctg ccgaccggct catcctcgat
     1561 ccggggatgg gatttttctt gagccccgca ccggaaacat cgctgcacgt gctgtcgaac
     1621 cttcaaaagc tgaagtcggc gttggggctt ccgctattgg tctcggtgtc gcggaaatcc
     1681 ttcttgggcg ccaccgttgg ccttcctgta aaggatctgg gtccagcgag ccttgcggcg
     1741 gaacttcacg cgatcggcaa tggcgctgac tacgtccgca cccacgcgcc tggagatctg
     1801 cgaagcgcaa tcaccttctc ggaaaccctc gcgaaatttc gcagtcgcga cgccagagac
     1861 cgagggttag atcatgcctg aaagcgacag gccatgtttg acagcttatc atcgataagc
     1921 tttaatgcgg tagtttatca cagttaaatt gctaacgcaa tgggtctcgg cctcatcatg
     1981 cccgtccttc cgacgcttct gcgtgagctt gtgccagcag agcaggtcgc tggacactat
     2041 ggtgccttgc tgtcgctcta tgcattgatg caggtcgtct tcgcgcccat gcttggacag
     2101 ctttcggatt cttacggtcg gcgtccggta cttctggctt ctcttgcagg agccgcagtc
     2161 gattacacga ttatggcatc agcgccggtc ttatgggtgc tctatatcgg ccgactcgtg
     2221 tccggcgtca cgggcgcaac cggagctgta gcagcctcaa ccattgccga ttcgacgggg
     2281 gaaggttctc gcgcacgctg gttcggctac atgggggcct gttatggggc gggcatgatt
     2341 gccgggccag cacttggtgg catgctcggt ggtatctctg ctcatgcccc gtttatcgcc
     2401 gccgcccttc tcaacgggtt cgcgttcctg cttgcctgca ttttcctcaa ggagactcat
     2461 cacagccatg gcgggaccgg aaagccggtt cgcatcaaac cattcgttct gttacggctg
     2521 gatgatgcat tgcgcgggct aggtgcgctt ttcgcagttt tcttcattat tcaactgatc
     2581 ggccaagtgc ctgcagccct atgggtcata tatggcgagg accgttttca gtggaacacc
     2641 gcgaccgttg gtttgtcgct cgcggcgttt ggggcaacac atgcgatctt ccaagcgttt
     2701 gttaccggcc cgctttcaag ccggcttgga gagcggcgca cgctgctgtt tggcatggct
     2761 gcggatgcga ctggcttcgt tcttctggct tttgccacgc agggatggat ggtgttcccg
     2821 attctgttgc tgcttgccgc cgggggtgtt ggcatgccgg ccttgcaggc aatgctctca
     2881 aacaatgtca gcagtaacaa gcaaggggct ttgcaaggaa cgctaacgag cctcaccaat
     2941 ctaagctcta tcgcaggacc gcttggcttc acagcactct attctgccac cgccggggca
     3001 tggaacggtt gggtttggat tgtcggcgcg atcctctatt taatatgtct gccaatacta
     3061 cgcagaccat tcgcaacttc attgtgagaa ttcactggcc gtcgttttac aacgtcgtga
     3121 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     3181 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     3241 tggcgaatgg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     3301 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     3361 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     3421 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     3481 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     3541 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     3601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     3661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     3721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     3781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     3841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     3901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     3961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     4021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     4081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     4141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     4201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     4261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     4321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     4381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     4441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     4501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     4561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     4621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     4681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     4741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     4801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     4861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     4921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     5041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     5101 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     5161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     5221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     5281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     5341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     5401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     5461 cagcgagtca gtgagcgagg aagcggaaga
//
