LOCUS       V014234                 5399 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014234
VERSION     V014234
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             219..935
                     /label="EGFP"
                     /note="enhanced GFP"
     CDS             951..1787
                     /gene="sulI"
                     /label="Dihydropteroate synthase type-1"
                     /note="Dihydropteroate synthase type-1 from Escherichia
                     coli. Accession#: P0C002"
     promoter        1807..1835
                     /label="tet promoter"
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     primer_bind     complement(3003..3019)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        3493..3597
                     /label="AmpR promoter"
     CDS             3598..4455
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4629..5217
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgattacg ccaagcttat ggtgagcaag ggcgaggagc
      241 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      301 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      361 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      421 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      481 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      541 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      601 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
      661 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
      721 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
      781 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
      841 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
      901 ccgggatcac tctcggcatg gacgagctgt acaagggtgg aggtggatca atggtgacgg
      961 tgttcggcat tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc
     1021 ccgccggcgc tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg
     1081 tcggaccggc cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac
     1141 gtattgcgcc gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct
     1201 tccaaccgga aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc
     1261 aaggatttcc tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg
     1321 ttatgcactc agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag
     1381 acgcgctcga cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg
     1441 gggtcgctgc cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac
     1501 cggaaacatc gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc
     1561 cgctattggt ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa
     1621 aggatctggg tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact
     1681 acgtccgcac ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg
     1741 cgaaatttcg cagtcgcgac gccagagacc gagggttaga tcatgcctga aagcgacagg
     1801 ccatgtttga cagcttatca tcgataagct ttaatgcggt agtttatcac agttaaattg
     1861 ctaacgcaat gggtctcggc ctcatcatgc ccgtccttcc gacgcttctg cgtgagcttg
     1921 tgccagcaga gcaggtcgct ggacactatg gtgccttgct gtcgctctat gcattgatgc
     1981 aggtcgtctt cgcgcccatg cttggacagc tttcggattc ttacggtcgg cgtccggtac
     2041 ttctggcttc tcttgcagga gccgcagtcg attacacgat tatggcatca gcgccggtct
     2101 tatgggtgct ctatatcggc cgactcgtgt ccggcgtcac gggcgcaacc ggagctgtag
     2161 cagcctcaac cattgccgat tcgacggggg aaggttctcg cgcacgctgg ttcggctaca
     2221 tgggggcctg ttatggggcg ggcatgattg ccgggccagc acttggtggc atgctcggtg
     2281 gtatctctgc tcatgccccg tttatcgccg ccgcccttct caacgggttc gcgttcctgc
     2341 ttgcctgcat tttcctcaag gagactcatc acagccatgg cgggaccgga aagccggttc
     2401 gcatcaaacc attcgttctg ttacggctgg atgatgcatt gcgcgggcta ggtgcgcttt
     2461 tcgcagtttt cttcattatt caactgatcg gccaagtgcc tgcagcccta tgggtcatat
     2521 atggcgagga ccgttttcag tggaacaccg cgaccgttgg tttgtcgctc gcggcgtttg
     2581 gggcaacaca tgcgatcttc caagcgtttg ttaccggccc gctttcaagc cggcttggag
     2641 agcggcgcac gctgctgttt ggcatggctg cggatgcgac tggcttcgtt cttctggctt
     2701 ttgccacgca gggatggatg gtgttcccga ttctgttgct gcttgccgcc gggggtgttg
     2761 gcatgccggc cttgcaggca atgctctcaa acaatgtcag cagtaacaag caaggggctt
     2821 tgcaaggaac gctaacgagc ctcaccaatc taagctctat cgcaggaccg cttggcttca
     2881 cagcactcta ttctgccacc gccggggcat ggaacggttg ggtttggatt gtcggcgcga
     2941 tcctctattt aatatgtctg ccaatactac gcagaccatt cgcaacttca ttgtgagaat
     3001 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     3061 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     3121 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     3181 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     3241 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     3301 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     3361 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3421 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     4021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     4081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     4141 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     4201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     4261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     4321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     4381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4741 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     4801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     5041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     5101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     5161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     5221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     5281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     5341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaaga
//
