LOCUS       V014643                 5563 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014643
VERSION     V014643
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5563)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(230..307)
                     /label="lacI promoter"
     CDS             complement(479..1267)
                     /gene="nanR"
                     /label="HTH-type transcriptional repressor NanR"
                     /note="HTH-type transcriptional repressor NanR from
                     Escherichia coli (strain K12). Accession#: P0A8W0"
     CDS             complement(1268..1285)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     misc_feature    complement(1352..1373)
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     CDS             1575..2240
                     /label="CopGFP"
                     /note="green fluorescent protein 2 from Pontellina plumata,
                     also known as ppluGFP2 (Shagin et al., 2004)"
     CDS             complement(2655..3311)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3312..3414)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3940..4485)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     protein_bind    complement(4676..4697)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(join(4713..5563,1..229))
                     /label="lacI"
                     /note="lac repressor"
ORIGIN
        1 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
       61 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
      121 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
      181 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
      241 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
      301 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa atcaataaaa
      361 ggacagtcat tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac
      421 gtaaatcagg tgaaactgac cgaccaatac ccttcaaacc gctagcccat ggttattatt
      481 tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc agagacgctg ttgagatgcg
      541 attgcaacgc acgatcggct tcgtcaggat catgacggcg gatcgcatca acgatcgcaa
      601 tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta accgttgggc
      661 gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc catgaagatt gggttaccgg
      721 ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc gcgttgttat
      781 ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc tcatcggtgg
      841 catgttcagc cgcatagcgc accagactgg attcaaagaa cagacgtaat tgttcgaaat
      901 gggcaatccc accgggatga gaaaggaaat ctttcgccat gccggaaagc tcaccgatga
      961 tagtgtccgc agaaggacgc gagacgcgag cgcgttcgcc gttgtttatt tgcaccagac
     1021 ctttgcgttt taacgctgcc agcgcttcac gcaccgaagg acgcccgacg ttaaagaacg
     1081 ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga cggcggatca
     1141 tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc gggcggctac
     1201 gcaagttgcg accaattgca ggtgaagaat cttcggtttg cgaatcaaat gcgttcataa
     1261 ggcccatatg gtgatggtga tgatgcatct agtaggtttc ctgtgtgact ctagtagcta
     1321 gcattgtacc taggactgag ctagccataa actctagaag cggccgcgaa ttcttgaaga
     1381 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     1441 tagacgtcag gtggcagctc cagcctacac caattggatt cgttaccaat tgacagctac
     1501 tggtataaca ggtataaagg tagctactag agaaagagga gaaatactag atggagagcg
     1561 acgagagcgg cctgatgccc gccatggaga tcgagtgccg catcaccggc accctgaacg
     1621 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca
     1681 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga
     1741 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc
     1801 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg
     1861 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg
     1921 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca
     1981 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc
     2041 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact
     2101 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc
     2161 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca
     2221 agacccccat cgccttcgcc taaagatccc gcgctcagtc gtccaattct gccgtggacg
     2281 gcaccgccgg acccggctcc accggatctc gcccatgggc tagcggtttg aagggtattg
     2341 gtcggtcagt ttcacctgat ttacgtaaaa acccgcttcg gcgggttttt gcttttggag
     2401 gggcagaaag atgaatgact gtccttttat tctgaaacct caggcatttg agaagcacac
     2461 ggtcacactg cttccggtag tcaataaacc ggtaaaccag caatagacat aagcggctat
     2521 ttaacgaccc tgccctgaac cgacgaccgg gtcgaatttg ctttcgaatt tctgccattc
     2581 atccgcttat tatcacttat tcaggcgtag caaccaggcg tttaagggca ccaataactg
     2641 ccttaaaaaa attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc
     2701 attctgccga catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc
     2761 agcaccttgt cgccttgcgt ataatatttg cccatagtga aaacgggggc gaagaagttg
     2821 tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg
     2881 aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc
     2941 acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc
     3001 gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat
     3061 atcaccagct caccgtcttt cattgccata cggaactccg gatgagcatt catcaggcgg
     3121 gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa
     3181 aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat
     3241 gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt
     3301 tttttctcca ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc
     3361 ggtagtgatc ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct
     3421 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
     3481 tattctgcga agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat
     3541 gctgccaact tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt
     3601 ttctatcagc tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac
     3661 cgccggacat cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt
     3721 gtcagtgaag tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata
     3781 tgtgatacag gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     3841 tgcggcgagc ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga
     3901 tacttaacag ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc
     3961 tgacaagcat cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata
     4021 aagataccag gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg
     4081 gtttaccggt gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca
     4141 gttccgggta ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     4201 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag
     4261 caccactggc agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc
     4321 ggttaaggct aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct
     4381 cggttcaaag agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt
     4441 tcgttttcag agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt
     4501 aatcagataa aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa
     4561 gtcagcccca tacgatataa gttgtaattc tcatgttagt catgccccgc gcccaccgga
     4621 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag
     4681 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4741 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4801 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc
     4861 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc
     4921 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc
     4981 actaccgaga tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc
     5041 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt
     5101 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc
     5161 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca
     5221 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc
     5281 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca
     5341 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc
     5401 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc
     5461 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca
     5521 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcg
//
