LOCUS       V007219                11808 bp    DNA     circular SYN 08-SEP-2021
DEFINITION  Exported.
ACCESSION   V007219
VERSION     V007219
KEYWORDS    pInducer20
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE,
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF,
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi:
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
   PUBMED   21307310
REFERENCE   2  (bases 1 to 11808)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11808)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A";
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(75..818)
                     /label="rtTA-Advanced"
                     /note="improved tetracycline-controlled transactivator"
     primer_bind     complement(1021..1040)
                     /label="hUBCpro-F"
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    complement(1255..1811)
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             complement(2174..2200)
                     /label="HA"
                     /note="HA (human influenza hemagglutinin) epitope tag"
     protein_bind    2253..2377
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(2421..2723)
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(3068..3724)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(3778..3808)
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(3833..3957)
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4066..4090)
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     promoter        complement(4087..4125)
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     protein_bind    complement(4158..4428)
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     misc_feature    complement(4483..4600)
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(4703..4744)
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     CDS             complement(4893..4937)
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     misc_feature    complement(5122..5355)
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     misc_feature    complement(5852..5977)
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     LTR             complement(6024..6657)
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6849..6868)
                     /label="EBV-rev"
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6918..6934)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(6918..6934)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6931..6953)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    6942..6958
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6966..6996)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7011..7032)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(7161..7180)
                     /label="SV40pro-F"
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(7272..7289)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7443..8031)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8205..9062)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9063..9167)
                     /label="AmpR promoter"
     primer_bind     9235..9253
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     LTR             complement(9372..9605)
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    complement(9680..10268)
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(10448..11239)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     misc_feature    complement(11255..11805)
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ggggggggag ggagaggggg gggggggaga ggggcggaat tcctctagtg cggccgcgga
       61 tccttactta gttacccggg gagcatgtca aggtcaaaat cgtcaagagc gtcagcaggc
      121 agcatatcaa ggtcaaagtc gtcaagggca tcggctggga gcatgtctaa gtcaaaatcg
      181 tcaagggcgt cggtcggccc gccgctttcg cactttagct gtttctccag gccacatatg
      241 attagttcca ggccgaaaag gaaggcaggt tcggctccct gccggtcgaa cagctcaatt
      301 gcttgtctca gaagtggggg catagaatcg gtggtaggtg tctctctttc ctcttttgct
      361 acttgatgct cctgttcctc caatacgcag cccagtgtaa agtggcccac ggcggacaga
      421 gcgtacagtg cgttctccag ggagaagcct tgctgacaca ggaacgcgag ctgattttcc
      481 agggtttcgt actgtttctc tgttgggcgg gtgccgagat gcactttagc cccgtcgcga
      541 tgtgagagga gagcacagcg gtatgacttg gcgttgttcc gcagaaagtc ttgccatgac
      601 tcgccttcca gggggcagaa gtgggtatga tgcctgtcca gcatctcgat tggcagggca
      661 tcgagcaggg cccgcttgtt cttcacgtgc cagtacaggg taggctgctc aactcccagc
      721 ttttgagcga gtttccttgt cgtcaggcct tcgataccga caccattgag taattccaga
      781 gctccgttta tgactttgct cttgtccagt ctagacatgg tgaattcgac tgcaggaccg
      841 gtacggggtg gagatccgag ctcggtacca agcttcgtct aacaaaaaag ccaaaaacgg
      901 ccagaattta gcggacaatt tactagtcta acactgaaaa ttacatattg acccaaatga
      961 ttacatttca aaaggtgcct aaaaaacttc acaaaacaca ctcgccaacc ccgagcgcat
     1021 agttcaaaac cggagcttca gctacttaag aagataggta cataaaaccg accaaagaaa
     1081 ctgacgcctc acttatccct cccctcacca gaggtccggc gcctgtcgat tcaggagagc
     1141 ctaccctagg cccgaaccct gcgtcctgcg acggagaaaa gcctaccgca cacctaccgg
     1201 caggtggccc caccctgcat tataagccaa cagaacgggt gacgtcacga cacgacgagg
     1261 gcgcgcgctc ccaaaggtac gggtgcactg cccaacggca ccgccataac tgccgccccc
     1321 gcaacagacg acaaaccgag ttctccagtc agtgacaaac ttcacgtcag ggtccccaga
     1381 tggtgcccca gcccatctca cccgaataag agctttcccg cattagcgaa ggcctcaaga
     1441 ccttgggttc ttgccgccca ccatgccccc caccttgttt caacgacctc acagcccgcc
     1501 tcacaagcgt cttccattca agactcggga acagccgcca ttttgctgcg ctccccccaa
     1561 cccccagttc agggcaacct tgctcgcgga cccagactac agcccttggc ggtctctcca
     1621 cacgcttccg tcccaccgag cggcccggcg gccacgaaag ccccggccag cccagcagcc
     1681 cgctactcac caagtgacga tcacagcgat ccacaaacaa gaaccgcgac ccaaatcccg
     1741 gctgcgacgg aactagctgt gccacacccg gcgcgtcctt atataatcat cggcgttcac
     1801 cgccccacgg agatccctcc gcagaatcgc cgagaaggga ctacttttcc tcgcctgttc
     1861 cgctctctgg aaagaaaacc agtgccctag agtcacccaa gtcccgtcct aaaatgtcct
     1921 tctgctgata ctggggttct aaggccgagt cttatgagca gcgggccgct gtcctgagcg
     1981 tccgggcgga aggatcagga cgctcgctgc gcccttcgtc tgacgtggca gcgctcgccg
     2041 tgaggagggg ggcgcccgcg ggaggcgcca aaacccggcg cggaggccag atcttgggtg
     2101 ggttactcca gactgccttg ggaaaagcgc ctcccctacc cggtagaatt tctagtttaa
     2161 ttaatcatta ctaagcgtag tctgggacgt cgtatgggta ttcgaaccgc gggccctcta
     2221 gactcgagcg gccgccactg tgctggatat caaccacttt gtacaagaaa gctgaacgag
     2281 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
     2341 cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt
     2401 ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca
     2461 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc
     2521 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
     2581 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
     2641 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
     2701 taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt
     2761 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
     2821 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
     2881 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
     2941 ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat attcttatac
     3001 cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatccag
     3061 atctttacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     3121 cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc atcagcacct
     3181 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     3241 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     3301 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     3361 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     3421 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     3481 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     3541 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     3601 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     3661 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     3721 ccattttagc ttccttagct cctgaaaatc tcgacggatc ctaactcaaa atccacacat
     3781 tatacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgcggcc gccatagtga
     3841 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     3901 tatattgata tttatatcat tttacgtttc tcgttcagct tttttgtaca aacttgttga
     3961 tatctgcaga attccaccac actggactag ttcggggccg cggaggctgg atcggtcccg
     4021 gtgtcttcta tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac
     4081 taaacgagct ctgcttatat aggcctccca ccgtacacgc ctacctcgac ccgggtaccg
     4141 agctcgactt tcacttttct ctatcactga tagggagtgg taaactcgac tttcactttt
     4201 ctctatcact gatagggagt ggtaaactcg actttcactt ttctctatca ctgataggga
     4261 gtggtaaact cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
     4321 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
     4381 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaaggat
     4441 cctagtccaa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     4501 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     4561 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     4621 ttgtgaattc ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct
     4681 ctctctccac cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg
     4741 tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca
     4801 gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac
     4861 cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat
     4921 aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag
     4981 cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt
     5041 tccagagatt tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt
     5101 ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg
     5161 attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc
     5221 tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc
     5281 tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca
     5341 aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc
     5401 ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata taattcactt
     5461 ctccaattgt ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct
     5521 gtgcggtggt cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt
     5581 cttttatctc tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc
     5641 taagttcttc tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc
     5701 cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc
     5761 atactatatg ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc
     5821 atcgcgatct aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct
     5881 agcctccgct agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg
     5941 tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt
     6001 tcaagtccct gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag
     6061 ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg
     6121 caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca
     6181 gatctggtct aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc
     6241 tgagggctcg ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg
     6301 aaagtccctt gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc
     6361 gggccacgtg atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg
     6421 ggtcatccat cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg
     6481 cctcttctac cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg
     6541 gatatctgac ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt
     6601 ggtagatcca cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact
     6661 actaagtttg tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag
     6721 gaatcacagg tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg
     6781 gtgggaagtt tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa
     6841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6901 gtgaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     6961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     7021 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     7081 cacggggagc tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa
     7141 tagctcagag gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg
     7201 agcttgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7261 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     7321 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     7381 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     7441 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     7501 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7561 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7621 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7681 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7741 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7801 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7861 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     7921 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7981 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     8041 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     8101 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     8161 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     8221 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     8281 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     8341 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     8401 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     8461 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     8521 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8581 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8641 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8701 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8761 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
     8821 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8881 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8941 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     9001 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     9061 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     9121 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     9181 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     9241 cgtatcacga ggccctttcg tcttcaagaa tgatctagcc ctttccttaa ttaacccggg
     9301 cctctcactc tctgatattc atttctttgc aagttataaa tactgaataa taagatgaca
     9361 tgaactacta ctgctagaga ttttccacac tgactaaaag ggtctgaggg atctctagtt
     9421 accagagtca cacaacagac gggcacacac tacttgaagc actcaaggca agctttattg
     9481 aggcttaagc agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa
     9541 ccagagagac ccagtacaag caaaaagcag atcttgtctt cgttgggagt gaattagccc
     9601 ttccagtccc cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat
     9661 tggtcttaaa ggatctcagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     9721 cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc
     9781 gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca
     9841 acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac ggaattgtca
     9901 gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc
     9961 gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg
    10021 ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca
    10081 acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg
    10141 aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc
    10201 gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga
    10261 ggttgattag gatctatcgc gataagcttg atatcgaatt gggagggact aaacaacaac
    10321 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag
    10381 taaaacctct acaaatgtgg tatggctgat tatgatctag agtcgcggcc gctttacttg
    10441 tacatcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
    10501 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
    10561 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
    10621 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
    10681 tcacgacgag atcctcgccg tcgggcatgc tcgccttgag cctggcgaac agttcggctg
    10741 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
    10801 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
    10861 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
    10921 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
    10981 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
    11041 gccgcgctgc ctcgtcttgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
    11101 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
    11161 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
    11221 atccatcttg ttcaatcatg gtggaccggt aagcttatca tcgtgttttt caaaggaaaa
    11281 ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa acacatgtaa
    11341 agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc tgggcatcct
    11401 tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca actatccaac
    11461 tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca cgtggctttt
    11521 ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg gtcgctacag
    11581 acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca ttcaacagac
    11641 cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag aagacagggc
    11701 caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat aacatataga
    11761 caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttag
//
