LOCUS       V010531                14567 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010531
VERSION     V010531
KEYWORDS    pInducer10-mir-RUP-PheS
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14567)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE,
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF,
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi:
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
   PUBMED   21307310
REFERENCE   2  (bases 1 to 14567)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14567)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A";
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    497..730
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             915..959
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1108..1149
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    1257..1374
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             complement(1442..1813)
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     promoter        complement(1833..1880)
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     protein_bind    1950..2220
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        2253..2291
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2288..2312
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             2388..3080
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     ncRNA           3134..3227
                     /label="5' miR-30a"
                     /note="sequence upstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     primer_bind     3227..3243
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
     primer_bind     3228..3244
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             3265..3282
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             3283..3306
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     regulatory      3316..3325
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3483..4463)
                     /gene="pheS"
                     /label="Phenylalanine--tRNA ligase alpha subunit"
                     /note="Phenylalanine--tRNA ligase alpha subunit from
                     Escherichia coli O139:H28 (strain E24377A / ETEC).
                     Accession#: A7ZMI2"
     CDS             4601..4621
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     ncRNA           4645..4757
                     /label="3' miR-30a"
                     /note="sequence downstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     promoter        4808..6019
                     /label="UbC promoter"
                     /note="human ubiquitin C promoter"
     protein_bind    6026..6059
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             6066..6809
                     /label="rtTA-Advanced"
                     /note="improved tetracycline-controlled transactivator"
     protein_bind    complement(6813..6846)
                     /label="loxP"
                     /note="Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6862..7444
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             7443..8036
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    8061..8649
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             8856..9089
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9196..9420
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9466..9894
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9903..9923)
                     /label="pBABE 3'"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10019..10229
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    11060..11181
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11230..11246)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(11230..11246)
                     /label="M13 Reverse"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11243..11265)
                     /label="M13/pUC Reverse"
                     /note="In lacZ gene"
     protein_bind    11254..11270
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11278..11308)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(11323..11344)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     primer_bind     complement(11461..11478)
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11632..12220)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(12394..13251)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(13252..13356)
                     /label="AmpR promoter"
     polyA_signal    13404..13538
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             13706..14339
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ccgagtaata ccgggggaga ttagatcgcg atgggaaaaa ttcggttaag gccaggggga
       61 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      121 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      181 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      241 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      301 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
      361 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
      421 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
      481 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
      541 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
      601 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
      661 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
      721 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
      781 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
      841 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
      901 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
      961 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     1021 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     1081 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     1141 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     1201 acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat ccacaatttt
     1261 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     1321 acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt
     1381 tattacaggg acagcagaga tccagtttgg ttagtaccgg gcccgctcta gtccggaatc
     1441 agtcctgctc ctcggccacg aagtgcacgc agttgccggc cgggtcgcgc agggcgaact
     1501 cccgccccca cggctgctcg ccgatctcgg tcatggccgg cccggaggcg tcccggaagt
     1561 tcgtggacac gacctccgac cactcggcgt acagctcgtc caggccgcgc acccacaccc
     1621 aggccagggt gttgtccggc accacctggt cctggaccgc gctgatgaac agggtcacgt
     1681 cgtcccggac cacaccggcg aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt
     1741 cggtccagaa ctcgaccgct ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg
     1801 tcaacttggc catggtggcc ctcctatagt gagtcgtatt atactatgcc gatatactat
     1861 gccgatgatt aattgtcaac acgtgctgca ggtccgaggt tctaggctag cttaaaggaa
     1921 ccaattcagt cgactggatc ctttaccact ccctatcagt gatagagaaa agtgaaagtc
     1981 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2041 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2101 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2161 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2221 aaagtgaaag tcgagctcgg tacccgggtc gaggtaggcg tgtacggtgg gaggcctata
     2281 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     2341 acctccatag aagacaccgg gaccgatcca gcctaccggt cgccaccatg agcgagctga
     2401 tcaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
     2461 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg aagatcaagg
     2521 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
     2581 gcagcaaagc cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
     2641 ctgagggctt cacatgggag agaatcacca catacgaaga cgggggcgtg ctgaccgcta
     2701 cccaggacac cagcttccag aacggctgca tcatctacaa cgtcaagatc aacggggtga
     2761 acttcccatc caacggccct gtgatgcaga agaaaacacg cggctgggag gccaacaccg
     2821 agatgctgta ccccgctgac ggcggcctga gaggccacag ccagatggcc ctgaagctcg
     2881 tgggcggggg ctacctgcac tgctccttca agaccacata cagatccaag aaacccgcta
     2941 agaacctcaa gatgcccggc ttccacttcg tggaccacag actggaaaga atcaaggagg
     3001 ccgacaaaga gacctacgtc gagcagcacg agatggctgt ggccaagtac tgcgacctcc
     3061 ctagcaaact ggggcacaga tgatgcggcc gcaagccttg ttaagtgctc gcttcggcag
     3121 cacatatact atgttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
     3181 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga ggtcgacggt
     3241 atcgataagc ttgacgtcca tatgctggta ccccgtggaa gtctggaagt tctcttccag
     3301 gggcccgaag gagacgccac catgggtcta gatagggata acagggtaat ggtaccggat
     3361 cgatccccca acttccaggc ccgccatagt gatttgattt gccagcgcat cgctatcaat
     3421 cgccgggttc acccattcgc gtaaccacag ttcactgaat ttcataatct attcctgcct
     3481 tatttaaact gtttgaggaa acgcagatcg ttttcgaaga atgaacgcag gtcggtgacg
     3541 ccgtaacgca acatagtcag acgctccatc cccatcccga agccgaaacc agagtaaact
     3601 tccgggtcga tgccaacgtt acgcaacacg ttcggatgca ccatcccgca gcccagcact
     3661 tccagccatt taccgttttt acccatgacg tccacttctg cagaaggttc ggtaaacggg
     3721 aagtaggaag gacggaagcg aatctgcaaa tcttcctcaa agaagttacg caggaagtcg
     3781 tgcagcgtgc ctttcaggtt ggtaaagctg atgttggtat caacaatcag accttccatc
     3841 tgatggaaca tcggcgtgtg agtctggtcg tagtcgttac gataaacacg gccaggcgcg
     3901 atgatacgaa tcggtggctg ctgggctttc atggtgcgga tctgtacgcc agaggtctgg
     3961 gtacgcagca ggcgggtagt gtcaaaccag aaagtgtcgt ggtcagcgcg cgccgggtgg
     4021 tgaccaggaa tgttcagagc atcgaagtta tgataatcgt cttcgatttc cggcccggtt
     4081 gccacggtaa agccaagctc accgaagaaa ctttcgatac ggtcgatggt acgggtaacc
     4141 ggatgcagac cgccgttttc aatgcgacga cctggcagag agacatcaat cgtttccgcc
     4201 gccagacgcg cattcagtgc agcgctttcc agttccgctt tacgcgcatt cagcgcctgc
     4261 tgaacctgct ctttcgcttc gttgataacc gcaccagctg ccggacgctc ttctggcggc
     4321 agctcacgca gggtcgtcat ctgaagggtt aagtgccctt ttttacccaa atattcgacg
     4381 cgcacattat ctaacgcggc aacatctgac gcctggctaa tggccgcctt cgcactggca
     4441 accagttctg cgagatgtga catggttttc ctcattgtgt cagtggtgac actggttcgt
     4501 tggacttaga gcctatccca tcaggctatt ttacttgcca ttttggtccc cgataccgtc
     4561 gaccgcggat taccctgtta tccctagatc tcacaattct gagaatttgt attttcaggg
     4621 tgatctccgt ggatctatta cgaattcaag gggctacttt aggagcaatt atcttgttta
     4681 ctaaaactga ataccttgct atctctttga tacattttta caaagctgaa ttaaaatggt
     4741 ataaattaaa tcactttttt caattggaag actaatgcgt ttaaacacgc ggcgacgcgt
     4801 aagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg
     4861 agcgctgcca cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc
     4921 aggacagcgg cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac
     4981 attttaggac gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca
     5041 ggcgaggaaa agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa
     5101 cgccgatgat tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga
     5161 tttgggtcgc ggttcttgtt tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg
     5221 ctgggctggc cggggctttc gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag
     5281 agaccgccaa gggctgtagt ctgggtccgc gagcaaggtt gccctgaact gggggttggg
     5341 gggagcgcag caaaatggcg gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg
     5401 gctgtgaggt cgttgaaaca aggtgggggg catggtgggc ggcaagaacc caaggtcttg
     5461 aggccttcgc taatgcggga aagctcttat tcgggtgaga tgggctgggg caccatctgg
     5521 ggaccctgac gtgaagtttg tcactgactg gagaactcgg tttgtcgtct gttgcggggg
     5581 cggcagttat ggcggtgccg ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg
     5641 tcgtgtcgtg acgtcacccg ttctgttggc ttataatgca gggtggggcc acctgccggt
     5701 aggtgtgcgg taggcttttc tccgtcgcag gacgcagggt tcgggcctag ggtaggctct
     5761 cctgaatcga caggcgccgg acctctggtg aggggaggga taagtgaggc gtcagtttct
     5821 ttggtcggtt ttatgtacct atcttcttaa gtagctgaag ctccggtttt gaactatgcg
     5881 ctcggggttg gcgagtgtgt tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt
     5941 tgggtcaata tgtaattttc agtgttagac tagtaaattg tccgctaaat tctggccgtt
     6001 tttggctttt ttgttagacg gatccataac ttcgtataat gtatgctata cgaagttatg
     6061 ccaccatgtc tagactggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     6121 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     6181 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     6241 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     6301 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     6361 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     6421 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     6481 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     6541 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     6601 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     6661 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     6721 tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct gacgctcttg
     6781 acgattttga ccttgacatg ctccccgggt aaataacttc gtatagcata cattatacga
     6841 agttatggcg cgccaattcc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
     6901 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
     6961 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
     7021 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
     7081 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
     7141 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
     7201 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
     7261 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
     7321 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
     7381 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccatgg
     7441 ccaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc cgggccgtac
     7501 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gacccggacc
     7561 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7621 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7681 gcgtcgaagc gggggcggtg ttcgccgaga tcggctcgcg catggccgag ttgagcggtt
     7741 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7801 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7861 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc tggggtgccc gccttcctgg
     7921 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7981 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat
     8041 taattaagcg cgtctggaac aatcaacctc tggattacaa aatttgtgaa agattgactg
     8101 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     8161 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     8221 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     8281 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     8341 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     8401 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
     8461 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     8521 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     8581 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     8641 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag
     8701 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     8761 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     8821 agatcttagc cactttttaa aagaaaagag gggactggaa gggctaattc actcccaacg
     8881 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     8941 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9001 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9061 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9121 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gtttaaaccc
     9181 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     9241 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     9301 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     9361 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     9421 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     9481 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9541 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9601 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9661 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     9721 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9781 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     9841 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     9901 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     9961 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10021 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10081 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10141 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
    10201 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
    10261 cattttcgga tctgatcagc acgtgatgaa aaagcctgaa ctcaccgcga cgtgaccgca
    10321 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
    10381 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
    10441 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
    10501 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
    10561 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
    10621 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
    10681 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
    10741 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
    10801 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
    10861 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
    10921 aaaggaatag cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    10981 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    11041 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
    11101 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11161 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
    11221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11281 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11341 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11401 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11461 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11521 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11581 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11641 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11701 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11761 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11821 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11881 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11941 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    12001 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12061 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12121 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12181 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12241 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12301 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12361 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12421 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12481 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12541 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12601 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12661 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12721 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12781 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    12841 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    12901 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    12961 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtccatac gggataatac
    13021 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13081 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13141 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13201 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13261 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13321 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    13381 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
    13441 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
    13501 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
    13561 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
    13621 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
    13681 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
    13741 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
    13801 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
    13861 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
    13921 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
    13981 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
    14041 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
    14101 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
    14161 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
    14221 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
    14281 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
    14341 tgccgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
    14401 actcggcttg ctgaagcgcg cacggcaaga ggcgaagggc ggcgactggt gagtacgcca
    14461 aaaattttga ctagcggaag gctagaagga agaagatggt gccagagcgt cagtattaag
    14521 cgggggaaaa ttagatcgcg aagggaaaaa attcggttaa ggccagg
//
