LOCUS       40924_25436        6882 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector pIgK3conalacZ, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 6882)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      953..1005
                     /label=IgK3
                     /note="IgK3"
                     /regulatory_class="promoter"
     regulatory      1009..1172
                     /label=CONA
                     /note="CONA"
                     /regulatory_class="promoter"
     CDS             1264..1284
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             1315..4359
                     /label=lacZ
                     /note="beta-galactosidase"
     polyA_signal    4510..4644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(4694..4712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4733..4749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4757..4773)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4781..4811)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4826..4847)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5135..5723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5897..6754)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6755..6859)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      721 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      781 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      841 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      901 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctgggga
      961 ttccccatga tctggggatt ccccatgatc tggggattcc ccagatccct attgagaaac
     1021 aaggaaggac aaacagcgtt aggtcattgc ttctgcaaac acagccaggg ctgctcctct
     1081 ataaaagggg aagaaagagg ctccgcagcc atcacagacc cagaggggac ggtctgtgac
     1141 caacaccgct gctccccctc ctcaacaccc aggatcgatc ctattccaga agtagtgaag
     1201 aggctttttt ggaggcctag gcttttgcaa aaagcttgat atcgaattcc tgcagccatg
     1261 gatccaaaaa agaagagaaa ggtagaagac ccccgcatgg ctcgcgatga tcccgtcgtt
     1321 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     1381 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     1441 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
     1501 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
     1561 cagatgcacg gttacgatgc gcccatctac accaacgtaa cctatcccat tacggtcaat
     1621 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
     1681 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
     1741 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
     1801 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgt
     1861 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
     1921 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
     1981 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
     2041 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
     2101 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
     2161 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
     2221 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
     2281 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
     2341 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
     2401 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
     2461 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
     2521 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
     2581 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
     2641 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
     2701 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
     2761 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
     2821 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
     2881 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
     2941 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
     3001 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
     3061 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
     3121 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
     3181 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
     3241 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
     3301 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
     3361 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
     3421 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
     3481 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
     3541 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
     3601 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
     3661 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
     3721 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
     3781 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
     3841 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
     3901 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
     3961 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
     4021 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
     4081 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
     4141 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
     4201 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
     4261 atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
     4321 gccggtcgct accattacca gttggtctgg tgtcaaaaat aataataacc gggcaggggg
     4381 gatctaagct ctagataagt aatgatcata atcagccata tcacatctgt agaggtttta
     4441 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4501 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4621 aatgtatctt atcatgtctg gatccactag ttctagagcg gccgccaccg cggtggagct
     4681 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     4741 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     4801 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     4861 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     4921 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4981 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5041 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5101 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5161 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5221 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5281 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5341 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5401 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5461 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5521 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     5581 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5641 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5701 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5761 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5821 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5881 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5941 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6001 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6061 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6121 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6181 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6241 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6301 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6361 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6421 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6481 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6541 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6601 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6661 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6721 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6781 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6841 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
