LOCUS       pIZT_V5-His.        3336 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Insect cell vector with a GFP-Zeocin(TM) resistance fusion gene, for
            constitutive expression of proteins with an optional C-terminal 
            V5-6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIZT V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3336
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..552
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    567..656
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             663..704
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             714..731
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    749..878
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1669..1960
                     /label=OpIE-1 promoter
                     /note="moderate constitutive baculovirus promoter for
                     insect cell expression"
     promoter        1986..2033
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2052..2054
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             2067..2771
                     /label=Cycle 3 GFP
                     /note="Cycle 3 GFP (Crameri et al., 1996)"
     CDS             2766..3143
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=Sh ble from Streptoalloteichus hindustanus
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
                     REFALRDPAGNCVHFVAEEQD"
     polyA_signal    3207..3336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
       61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
      121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
      181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
      241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
      301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
      361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
      421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
      481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
      541 acagcatctg ttcgaattta aagcttggta ccgagctcgg atccactagt ccagtgtggt
      601 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
      661 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
      721 accatcacca ttgagtttat ctgactaaat cttagtttgt attgtcatgt tttaatacaa
      781 tatgttatgt ttaaatatgt ttttaataaa ttttataaaa taatttcaac ttttattgta
      841 acaacattgt ccatttacac actcctttca agcgcgtggg atcgatgctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatgc gaaacacgca
     1681 cggcgcgcgc acgcagctta gcacaaacgc gtcgttgcac gcgcccaccg ctaaccgcag
     1741 gccaatcggt cggccggcct catatccgct caccagccgc gtcctatcgg gcgcggcttc
     1801 cgcgcccatt ttgaataaat aaacgataac gccgttggtg gcgtgaggca tgtaaaaggt
     1861 tacatcatta tcttgttcgc catccggttg gtataaatag acgttcatgt tggtttttgt
     1921 ttcagttgca agttggctgc ggcgcgcgca gcacctttgc cgggatctgc cgggctgcag
     1981 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     2041 ggaactaaac catggcggta gaaaaaatgg ctagcaaagg agaagaactt ttcactggag
     2101 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
     2161 gagagggtga aggtgatgct acatacggaa agcttaccct taaatttatt tgcactactg
     2221 gaaaactacc tgttccatgg ccaacacttg tcactacttt ctcttatggt gttcaatgct
     2281 tttcccgtta tccggatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
     2341 gttatgtaca ggaacgcact atatctttca aagatgacgg gaactacaag acgcgtgctg
     2401 aagtcaagtt tgaaggtgat acccttgtta atcgtatcga gttaaaaggt attgatttta
     2461 aagaagatgg aaacattctc ggacacaaac tcgagtacaa ctataactca cacaatgtat
     2521 acatcacggc agacaaacaa aagaatggaa tcaaagctaa cttcaaaatt cgtcacaaca
     2581 ttgaagatgg atccgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
     2641 gccctgtcct tttaccagac aaccattacc tgtcgacaca atctgccctt tcgaaagatc
     2701 ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt aactgctgct gggattacac
     2761 atggcatgga tgccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     2821 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     2881 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     2941 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     3001 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     3061 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     3121 tcgtggccga ggagcaggac tgaccgacgc cgaccaacac cgccggtccg acgcggcccg
     3181 acgggtccga ggggggtcga cctcgaaact tgtttattgc agcttataat ggttacaaat
     3241 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3301 gtttgtccaa actcatcaat gtatcttatc atgtct
//
