LOCUS       62056_13030        3688 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3688
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..552
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     CDS             567..1241
                     /codon_start=1
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     CDS             1475..1516
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     CDS             1526..1543
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    1561..1690
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1818..2406)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3054..3101
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             3121..3492
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    3559..3688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
       61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
      121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
      181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
      241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
      301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
      361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
      421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
      481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
      541 acagcatctg ttcgaattta aagcttatgg cctcctccga gaacgtcatc accgagttca
      601 tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg
      661 agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg
      721 gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt
      781 acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca
      841 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc caggactcct
      901 ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg
      961 acggccccgt gatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc
     1021 cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct gaagctgaag gacggcggcc
     1081 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
     1141 actactacgt ggacgccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
     1201 agcagtacga gcgcaccgag ggccgccacc acctgttcct gggtaccgag ctcggatccc
     1261 ccccgaagaa ctgtacccac ttaggcggct gtaatagcga ctgtctgacc cgtagcgaga
     1321 ttcaagcact gtttagagaa gcgataaaca cactgaaaca cacaatgaat actgaagacg
     1381 agtgcgctca catgttggat atagtgtcat tcgaaagaat caaggagtac attcgcgccg
     1441 cggccgctcg agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct
     1501 cggtctcgat tctacgcgta ccggtcatca tcaccatcac cattgagttt atctgactaa
     1561 atcttagttt gtattgtcat gttttaatac aatatgttat gtttaaatat gtttttaata
     1621 aattttataa aataatttca acttttattg taacaacatt gtccatttac acactccttt
     1681 caagcgcgtg ggatcgatgc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1741 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1801 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1861 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1921 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1981 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     2041 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     2101 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     2161 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     2221 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     2281 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     2341 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2401 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2461 cgttaaggga ttttggtcat gatgataaac aatgtatggt gctaatgttg cttcaacaac
     2521 aattctgttg aactgtgttt tcatgtttgc caacaagcac ctttatactc ggtggcctcc
     2581 ccaccaccaa cttttttgca ctgcaaaaaa acacgctttt gcacgcgggc ccatacatag
     2641 tacaaactct acgtttcgta gactatttta cataaatagt ctacaccgtt gtatacgctc
     2701 caaatacact accacacatt gaaccttttt gcagtgcaaa aaagtacgtg tcggcagtca
     2761 cgtaggccgg ccttatcggg tcgcgtcctg tcacgtacga atcacattat cggaccggac
     2821 gagtgttgtc ttatcgtgac aggacgccag cttcctgtgt tgctaaccgc agccggacgc
     2881 aactccttat cggaacagga cgcgcctcca tatcagccgc gcgttatctc atgcgcgtga
     2941 ccggacacga ggcgcccgtc ccgcttatcg cgcctataaa tacagcccgc aacgatctgg
     3001 taaacacagt tgaacagcat ctgttcgaat taattcggat ctctgcagca cgtgttgaca
     3061 attaatcatc ggcatagtat atcggcatag tataatacga ctcactatag gagggccacc
     3121 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     3181 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     3241 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     3301 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     3361 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     3421 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     3481 gaggagcagg actgaccgac gccgaccaac accgccggtc cgacggcggc ccacgggtcc
     3541 caggggggtc gacctcgaaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3601 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3661 aaactcatca atgtatctta tcatgtct
//
