LOCUS       pIZ_V5-His.        2876 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact insect cell vector with a Zeocin(TM) resistance marker, for 
            constitutive expression of proteins with an optional C-terminal 
            V5-6xHis tag.
ACCESSION   .
VERSION     .
KEYWORDS    pIZ V5-His
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..552
                     /label=OpIE-2 promoter
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     misc_feature    561..656
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             663..704
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
     CDS             714..731
                     /label=6xHis
                     /note="6xHis affinity tag"
     polyA_signal    749..878
                     /label=OpIE-2 poly(A) signal
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1006..1594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1666..2213
                     /gene="immediate-early gene 2 from the Orgyia pseudotsugata
                     multicapsid nucleopolyhedrovirus"
                     /label=immediate-early gene 2 from the Orgyia pseudots
                     /note="OpIE-2 promoter"
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     promoter        2242..2289
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2309..2680
                     /label=BleoR
                     /note="antibiotic-binding protein"
     polyA_signal    2747..2876
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
       61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
      121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
      181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
      241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
      301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
      361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
      421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
      481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
      541 acagcatctg ttcgaattta aagcttggta ccgagctcgg atccactagt ccagtgtggt
      601 ggaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg
      661 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc
      721 accatcacca ttgagtttat ctgactaaat cttagtttgt attgtcatgt tttaatacaa
      781 tatgttatgt ttaaatatgt ttttaataaa ttttataaaa taatttcaac ttttattgta
      841 acaacattgt ccatttacac actcctttca agcgcgtggg atcgatgctc actcaaaggc
      901 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      961 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1021 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1081 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1141 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1201 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1261 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1321 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1381 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1441 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1501 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1561 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1621 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga tgataaacaa
     1681 tgtatggtgc taatgttgct tcaacaacaa ttctgttgaa ctgtgttttc atgtttgcca
     1741 acaagcacct ttatactcgg tggcctcccc accaccaact tttttgcact gcaaaaaaac
     1801 acgcttttgc acgcgggccc atacatagta caaactctac gtttcgtaga ctattttaca
     1861 taaatagtct acaccgttgt atacgctcca aatacactac cacacattga acctttttgc
     1921 agtgcaaaaa agtacgtgtc ggcagtcacg taggccggcc ttatcgggtc gcgtcctgtc
     1981 acgtacgaat cacattatcg gaccggacga gtgttgtctt atcgtgacag gacgccagct
     2041 tcctgtgttg ctaaccgcag ccggacgcaa ctccttatcg gaacaggacg cgcctccata
     2101 tcagccgcgc gttatctcat gcgcgtgacc ggacacgagg cgcccgtccc gcttatcgcg
     2161 cctataaata cagcccgcaa cgatctggta aacacagttg aacagcatct gttcgaatta
     2221 attcggatct ctgcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta
     2281 taatacgact cactatagga gggccaccat ggccaagttg accagtgccg ttccggtgct
     2341 caccgcgcgc gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg
     2401 ggacttcgtg gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag
     2461 cgcggtccag gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct
     2521 ggacgagctg tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg
     2581 gccggccatg accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc
     2641 ggccggcaac tgcgtgcact tcgtggccga ggagcaggac tgaccgacgc cgaccaacac
     2701 cgccggtccg acggcggccc acgggtccca ggggggtcga cctcgaaact tgtttattgc
     2761 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     2821 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtct
//
