LOCUS       pIX_3.0.        2697 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Prokaryotic in vitro translation vector for cloning PCR products 
            generated with the EasyXpress Linear Template Kit.
ACCESSION   .
VERSION     .
KEYWORDS    pIX 3.0
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The PCR products include regulatory elements for expression in 
            bacterial extracts, plus optional tag sequences.
FEATURES             Location/Qualifiers
     source          1..2697
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    406..461
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(476..492)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(500..516)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(524..554)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(569..590)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(878..1466)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1640..2497)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2498..2602)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctttctcga gcggccgcta
      421 gcatgccatg gatatcaggc ctggatccga attcaccggt gcaattcgta atcatgtcat
      481 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
      541 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
      601 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
      661 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
      721 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      781 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      841 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      901 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      961 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1021 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1081 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1141 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1201 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1261 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1321 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1381 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1441 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1501 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1561 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1621 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     1681 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     1741 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     1801 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1861 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1921 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1981 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2041 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2101 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2161 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2221 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     2281 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2341 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     2401 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     2461 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     2521 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     2581 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     2641 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
