LOCUS       62056_13020        3059 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        304..408
                     /label=AmpR promoter
     CDS             409..1266
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1440..2028
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2316..2337
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2352..2382
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2390..2406
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2414..2430
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2454..2472
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        2484..2502
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           2545..2587
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     misc_feature    complement(2594..2650)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     3'UTR           2688..2813
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     primer_bind     complement(2874..2890)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
       61 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      121 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      181 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      241 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      901 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
      961 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1021 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1081 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1141 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1201 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1261 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1321 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1561 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     1621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2221 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     2281 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     2341 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     2401 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctcca acgatttagg
     2461 tgacactata gaagagaagg aattaatacg actcactata gggagagaga gagaattacc
     2521 ctcactaaag ggaggagaag cttgcttgtt ctttttgcag aagctcagaa taaacgctca
     2581 actttggcag atcaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga
     2641 gctcgaattc gcccttctag tgactgacta ggatctggtt accactaaac cagcctcaag
     2701 aacacccgaa tggagtctct aagctacata ataccaactt acactttaca aaatgttgtc
     2761 ccccaaaatg tagccattcg tatctgctcc taataaaaag aaagtttctt cacattctaa
     2821 aaaaaaaaaa aaaaaaaaaa aaaaaaaaac cccccccccc caagggcgaa ttcactggcc
     2881 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     2941 gcacatcccc ctttcgccgc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     3001 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcat
//
