LOCUS       62056_13005        4103 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     5'UTR           8..50
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             108..1136
                     /codon_start=1
                     /label=Cre
                     /note="site-specific recombinase"
                     /translation="VSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     3'UTR           1195..1320
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus
                     laevis"
     primer_bind     complement(1381..1397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1870..1974
                     /label=AmpR promoter
     CDS             1975..2832
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3006..3594
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3882..3903
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3918..3948
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3956..3972
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3980..3996
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4020..4038
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        4050..4068
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 aagcttgctt gttctttttg cagaagctca gaataaacgc tcaactttgg cagatcaagc
       61 ttgcatgcct gcaggtcgac gccaccatgc ccaagaagaa gaggaaggtg tccaatttac
      121 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
      181 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
      241 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
      301 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
      361 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
      421 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
      481 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
      541 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
      601 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
      661 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
      721 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
      781 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
      841 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
      901 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
      961 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
     1021 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc atgaactata
     1081 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
     1141 gtaccgagct cgaattcgcc cttctagtga ctgactagga tctggttacc actaaaccag
     1201 cctcaagaac acccgaatgg agtctctaag ctacataata ccaacttaca ctttacaaaa
     1261 tgttgtcccc caaaatgtag ccattcgtat ctgctcctaa taaaaagaaa gtttcttcac
     1321 attctaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaacccc ccccccccaa gggcgaattc
     1381 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     1441 ccttgcagca catccccctt tcgccgctgg cgtaatagcg aagaggcccg caccgatcgc
     1501 ccttcccaac agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt
     1561 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     1621 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     1681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     1741 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     1801 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     1861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     1921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     1981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     2641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     2701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     2761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     2821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     2881 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     2941 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3001 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3061 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3121 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3181 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3241 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3301 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3361 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3421 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3481 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3541 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3601 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3661 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3721 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3781 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     3841 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     3901 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     3961 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctccaacga
     4021 tttaggtgac actatagaag agaaggaatt aatacgactc actataggga gagagagaga
     4081 attaccctca ctaaagggag gag
//
