LOCUS       62056_12990        4871 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             148..1797
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(1841..1962)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2381..2969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3143..4000)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4001..4105)
                     /label=AmpR promoter
     rep_origin      4132..4587
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4718..4766
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4780..4871
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 atcgataggt accgagctct tacgcgtgct agcgaaactg aaactgaaac tgaaactgaa
       61 actgaaactg aaactgaaac tgaaactgaa actagatctg ggtatataat ggaagcttgg
      121 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
      181 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
      241 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
      301 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
      361 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
      421 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
      481 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
      541 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
      601 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
      661 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
      721 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
      781 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
      841 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
      901 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
      961 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
     1021 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     1081 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
     1141 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
     1201 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     1261 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
     1321 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     1381 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     1441 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
     1501 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
     1561 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     1621 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
     1681 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     1741 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
     1801 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
     1861 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     1921 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     1981 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
     2041 tacaaatgtg gtaaaatcga taaggatccg tcgaccgatg cccttgagag ccttcaaccc
     2101 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     2161 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     2221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     3181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     3241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     3301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     3361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     3421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     3481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     3541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     3601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     3661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     3721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     3781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     3841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     3901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     3961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     4021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     4081 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
     4141 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4201 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4261 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4321 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4381 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4441 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4501 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4561 ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg
     4621 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa
     4681 gtaatattaa ggtacgggag gtacttggag cggccgcaat aaaatatctt tattttcatt
     4741 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa
     4801 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag
     4861 aacatttctc t
//
