LOCUS       62056_12985        3868 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3868
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(993..1011)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1029..1045)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1053..1069)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1077..1107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1122..1143)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1431..2019)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2193..3050)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3051..3155)
                     /label=AmpR promoter
     rep_origin      complement(3233..3688)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3829..3845
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3852..3868
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat
       61 tctcggtcct tctgctccag agcaatatgg gccacgccag gagctcaaac tattctcttc
      121 caggcctcac agcctcccag cccagccacg gtctgcaagc ccatcagcac cagctccagg
      181 actctctgct gggcccactc acctccagtt tggtggactt gggttcctaa gtggggagat
      241 attggggcct tgaagggatg gccggagcag caaccaaccg cagcaatcta ggggacactt
      301 gtacatagaa gccagggaca attctgcagc ttgtttctgg ggttctttgc gcagaaagga
      361 accacaggct ttcacaattg tctttttaaa aattacaacc aacagcggtc tcagttgagc
      421 atcctctccc tcctctccaa ctcttaatac ccttgcattt cccctcccac taaaaggtca
      481 ggaaataaca aaaacagcca ggcttggttc tgggcaaccc aggcatcctt tacagtagtc
      541 ccttattcca cctcttcttc ctcctcctcc tcgtcttctt ttaatgaaca gaaattacaa
      601 atcagctgga ttgtaattat ttttttaatt tatttatgga gaaacggttt agggaagagg
      661 aaaaggaaat gaagagagat caaaataatg aaaacaagaa accttcagtg tgaggggaac
      721 tggctgcctt cttaagacaa ataggaaaac tataatcaca gctgtttttt gatgggaaga
      781 ttaagtttac atttttcata tttagtgtta aataatttta tgtagattaa aataaaagag
      841 cagtattagc aagggaaaca agtgcctaaa tgtcttaatg ctctctatgg gaggcggtta
      901 gggtagcagc gaaatcacta gtgaattcgc ggccgcctgc aggtcgacca tatgggagag
      961 ctcccaacgc gttggatgca tagcttgagt attctatagt gtcacctaaa tagcttggcg
     1021 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1081 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1141 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     1201 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     1261 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1321 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1381 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1441 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1501 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1561 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1621 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1681 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1741 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1801 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1861 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1921 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1981 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2041 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2101 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2161 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2221 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2281 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2341 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2401 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2461 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     2521 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2581 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2641 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2701 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     2761 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     2821 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2881 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2941 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3001 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3061 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3121 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3181 gatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta
     3241 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     3301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     3361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     3421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     3481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     3541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     3601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     3661 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     3721 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     3781 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     3841 ccagtgaatt gtaatacgac tcactata
//
