LOCUS       40924_25796        3851 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Bacterial expression vector pISA417, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman 
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent, 
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 3851)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3851)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927, 
            9052, BELGIUM"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(399..775)
                     /label=3' UTR of the cobA gene
                     /note="3' UTR of the cobA gene"
     CDS             complement(776..1549)
                     /codon_start=1
                     /note="unnamed protein product; cobA"
                     /protein_id="SJL88161.1"
                     /translation="MTTTLLPGTVTLVGAGPGDPELVTVAGLRAVQQAEVILYDRLAPQ
                     DLLSEASDDAELVPVGKIPRGHYVPQEEINQLLVAHAREGRKVVRLKGGDSFVFGRGGE
                     EWQACAEAGIPVRVIPGVSSATAGPALAGIPLTHRHLVQGFTVVSGHVSPSDERSEVPW
                     RQLAKDRLTLVILMGVAHMRDIAPELMAGGLPADTPVRVVSNASLASQESWRTTLGDAV
                     ADMDAHHVRPPALVVVGTLAGVDLSHPDHRAPSDH"
     misc_feature    complement(1550..1584)
                     /label=5' UTR of the cobA gene
                     /note="5' UTR of the cobA gene"
     primer_bind     complement(1630..1646)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1654..1670)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1678..1708)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1723..1744)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2032..2620)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2794..3651)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3652..3756)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgacgcgcgt ggcatccgat
      421 cggcgtgact ccccgcgcac cgtgaagccg acctcgcggc ccagggcggt gccggacagt
      481 tcggcgagtc ggtgggccgc cgcgcgggcg gcgatgcgcc ggggttgggt gaccacgacg
      541 cgcccggcat ggtgatgcgc ggccaggggc ggcatcacgg tggtcttgcc cgagcccgga
      601 ggggcctgca ccaccaggcg cgcatccggc gccgtgaggg ccgcggcgag ctcgtccagg
      661 cgcccaccaa ccggcagccc ggcggctatc cgatcgaggt caaacacgtt cggcagtgtc
      721 tcacggccgc aggccacgcc gatcgatcag caggtgcccg gcggccggcc gggactcagt
      781 ggtcgctggg cgcgcgatgg tcgggatgcg acaggtcgac gccggccagg gtacccacca
      841 ccaccagcgc gggcggacgc acgtggtgcg cgtccatgtc ggccacggca tcgcccagcg
      901 tggtgcgcca cgattcctgg ctggccaggc tcgcattgct caccacgcgc acgggggtgt
      961 cggcaggcag cccgccggcc atcaattccg gcgcgatgtc gcgcatatgg gccacgccca
     1021 tcaggatcac cagcgtgagc cggtccttgg cgagttggcg ccatggcacc tcggagcgct
     1081 cgtcgctggg cgatacatgc cccgacacga cggtgaaccc ctgcaccagg tggcgatggg
     1141 tcagcgggat gccggccagc gccgggcccg cggtggccga ggagactccc gggatcacgc
     1201 gcaccgggat gccggcctcg gcgcaggcct gccattcctc gccgccacgc ccgaagacga
     1261 acgagtcgcc acccttgagg cgcaccacct tgcggccctc gcgggcgtgc gcgacgagca
     1321 gttggttgat ctcctcctgg ggcacatagt ggccgcgcgg gatcttgccg accggcacga
     1381 gttcggcgtc gtcggacgcc tccgacagca ggtcctgcgg ggcgagccgg tcgtagagga
     1441 tcacctcggc ctgctgcacg gcccgcaggc cggccacggt gacgagttca gggtcgccgg
     1501 gcccggcgcc gacgagggtg acagtgccgg gcaacagtgt ggtggtcatc gggtttcctc
     1561 atcggtgttg gtggtgtcgt cgnnnnctct agagtcgacc tgcaggcatg caagcttggc
     1621 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1681 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1741 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1801 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1861 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1921 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1981 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2401 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2461 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2521 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2581 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2641 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2701 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2761 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2821 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2881 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2941 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3001 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3061 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3121 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3181 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3241 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3301 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3361 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3421 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3481 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3541 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3601 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3661 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3721 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3781 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     3841 gccctttcgt c
//
