LOCUS       40924_25781        6507 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast selection vector pIS420, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Sadowski I, Lourenco P, Parent J.
  TITLE     Dominant marker vectors for selecting yeast mating products
  JOURNAL   Yeast 25 (8), 595-599 (2008)
  PUBMED    18613257
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Sadowski I, Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2008) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 6507)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6507)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "8"; pages: "595-599"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-2008) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..845
                     /label=ARS1/trp1
                     /note="ARS1/trp1"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1245..1349
                     /label=AmpR promoter
     CDS             1350..2207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2381..2969
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3227..3245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(3353..4928)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     CDS             complement(5485..6285)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6286..6502)
                     /label=URA3 promoter
     rep_origin      6504..6507
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
       61 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
      121 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
      181 ttaagtattg tttgtgcact tgcctgcagg ccttttgaaa agcaagcata aaagatctaa
      241 acataaaatc tgtaaaataa caagatgtaa agataatgct aaatcatttg gctttttgat
      301 tgattgtaca ggaaaatata catcgcaggg ggttgacttt taccatttca ccgcaatgga
      361 atcaaacttg ttgaagagaa tgttcacagg cgcatacgct acaatgaccc gattcttgct
      421 agccttttct cggtcttgca aacaaccgcc ggcagcttag tatataaata cacatgtaca
      481 tacctctctc cgtatcctcg taatcatttt cttgtattta tcgtcttttc gctgtaaaaa
      541 ctttatcaca cttatctcaa atacacttat taaccgcttt tactattatc ttctacgctg
      601 acagtaatat caaacagtga cacatattaa acacagtggt ttctttgcat aaacaccatc
      661 agcctcaagt cgtcaagtaa agatttcgtg ttcatgcaga tagataacaa tctatatgtt
      721 gataattagc gttgcctcat caatgcgaga tccgtttaac cggaccctag tgcacttacc
      781 ccacgttcgg tccactgtgt gccgaacatg ctccttcact attttaacat gtgggaattc
      841 ctcgacctgc agcgtacgaa gcttcagctg gcggccgcgt tctatagtgt cacctaaatc
      901 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
      961 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3181 cgcgttggcc gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact
     3241 atagggagac cggcagatcc gcggccgcat aggccactag tggatctgat atcatcgatg
     3301 aattcgagct cgttttcgac actggatggc ggcgttagta tcgaatcgac agcagtatag
     3361 cgaccagcat tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat
     3421 ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata
     3481 gaacaactac aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt
     3541 tattgtcagt actgattatt cctttgccct cggacgagtg ctggggcgtc ggtttccact
     3601 atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc gggcgatttg
     3661 tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac cctgcgccca
     3721 agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa gaccaatgcg
     3781 gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc gctcgaagta
     3841 gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt tggcgacctc
     3901 gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta tgcggccatt
     3961 gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga cttcggggca
     4021 gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac tgacggtgtc
     4081 gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata tgaaatcacg
     4141 ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc tcgtctggct
     4201 aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa cagcgggcag
     4261 ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga tgcaataggt
     4321 caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg cggccgatgc
     4381 aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat ttacccgcag
     4441 gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc cctccgagag
     4501 ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga cagacgtcgc
     4561 ggtgagttca ggctttttac ccatggttgt ttatgttcgg atgtgatgtg agaactgtat
     4621 cctagcaaga ttttaaaagg aagtatatga aagaagaacc tcagtggcaa atcctaacct
     4681 tttatatttc tctacagggg cgcggcgtgg ggacaattca acgcgtctgt gaggggagcg
     4741 tttccctgct cgcaggtctg cagcgaggag ccgtaatttt tgcttcgcgc cgtgcggcca
     4801 tcaaaatgta tggatgcaaa tgattataca tggggatgta tgggctaaat gtacgggcga
     4861 cagtcacatc atgcccctga gctgcgcacg tcaagactgt caaggagggt attctgggcc
     4921 tccatgtcgc tggccgggtg acccggcggg gacgaggcaa gctaaacaga tctggcgcgc
     4981 cttaattaac ccggggatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt
     5041 ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc
     5101 gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg
     5161 actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac
     5221 caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt
     5281 agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta
     5341 aagctttttc tttccaattt tttttttttc gtcattatag aaatcattac gaccgagatt
     5401 cccgggtaat aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc
     5461 atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca
     5521 gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc
     5581 tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg
     5641 ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc
     5701 gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt
     5761 gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagcta gggagccctt
     5821 gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttccgccgc
     5881 ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc
     5941 ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc
     6001 agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg
     6061 cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca
     6121 aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa
     6181 tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg
     6241 actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt
     6301 cctgcaggtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
     6361 aacaccacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     6421 ctgctcggag attaccgaat caaaaaaatt tcaaagaaac cggaatcaaa aaaaagaaca
     6481 aaaaaaaaaa agatgaattg aaaagct
//
