LOCUS       40924_25776        6054 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast selection vector pIS419, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Sadowski I, Lourenco P, Parent J.
  TITLE     Dominant marker vectors for selecting yeast mating products
  JOURNAL   Yeast 25 (8), 595-599 (2008)
  PUBMED    18613257
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Sadowski I, Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2008) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 6054)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6054)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "8"; pages: "595-599"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-2008) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..845
                     /label=ARS1/trp1
                     /note="ARS1/trp1"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1245..1349
                     /label=AmpR promoter
     CDS             1350..2207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2381..2969
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3227..3245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(3353..4475)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     CDS             complement(5032..5832)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5833..6049)
                     /label=URA3 promoter
     rep_origin      6051..6054
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
       61 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
      121 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
      181 ttaagtattg tttgtgcact tgcctgcagg ccttttgaaa agcaagcata aaagatctaa
      241 acataaaatc tgtaaaataa caagatgtaa agataatgct aaatcatttg gctttttgat
      301 tgattgtaca ggaaaatata catcgcaggg ggttgacttt taccatttca ccgcaatgga
      361 atcaaacttg ttgaagagaa tgttcacagg cgcatacgct acaatgaccc gattcttgct
      421 agccttttct cggtcttgca aacaaccgcc ggcagcttag tatataaata cacatgtaca
      481 tacctctctc cgtatcctcg taatcatttt cttgtattta tcgtcttttc gctgtaaaaa
      541 ctttatcaca cttatctcaa atacacttat taaccgcttt tactattatc ttctacgctg
      601 acagtaatat caaacagtga cacatattaa acacagtggt ttctttgcat aaacaccatc
      661 agcctcaagt cgtcaagtaa agatttcgtg ttcatgcaga tagataacaa tctatatgtt
      721 gataattagc gttgcctcat caatgcgaga tccgtttaac cggaccctag tgcacttacc
      781 ccacgttcgg tccactgtgt gccgaacatg ctccttcact attttaacat gtgggaattc
      841 ctcgacctgc agcgtacgaa gcttcagctg gcggccgcgt tctatagtgt cacctaaatc
      901 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
      961 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3181 cgcgttggcc gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact
     3241 atagggagac cggcagatcc gcggccgcat aggccactag tggatctgat atcatcgatg
     3301 aattcgagct cgttttcgac actggatggc ggcgttagta tcgaatcgac agcagtatag
     3361 cgaccagcat tcacatacga ttgacgcatg atattacttt ctgcgcactt aacttcgcat
     3421 ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac gctaacattt gattaaaata
     3481 gaacaactac aatataaaaa aactatacaa atgacaagtt cttgaaaaca agaatctttt
     3541 tattgtcagt actgattagg ggcagggcat gctcatgtag agcgcctgcc gctcgccgtc
     3601 cgaggcggtg ccgtcgtaca gggcggtgtc caggccgcag agggtgaacc ccatccgccg
     3661 gtacgcgtgg atcgccggtg cgttgacgtt ggtgacctcc agccagaggt gcccggcgcc
     3721 ccgctcgccg gcgaactccg tcgcgagccc catcaacgcg cgcccgaccc cgtgcccccg
     3781 gtgctccggg gcgacctcga tgtcctcgac ggtcagccgg cggttccacg ccgagtacga
     3841 gatgaccacg aagcccgcca ggtcgccgtc gtccccgtac gcgacgaacg tccgggagtc
     3901 cgggtcgccg tcctccccgt cgtccgattc gtcgtccgat tcgtcgtcgg ggaacacctt
     3961 ggtcaggggc gggtccaccg gcacctcccg cagggtgaag ccgtccccgg tggcggtgac
     4021 gcggaagacg gtgtcggtgg tgaaggaccc atccagtgcc tcgatggcct cggcgtcccc
     4081 cgggacactg gtgcggtacc ggtaagccgt gtcgtcaaga gtggtaccca tggttgttta
     4141 tgttcggatg tgatgtgaga actgtatcct agcaagattt taaaaggaag tatatgaaag
     4201 aagaacctca gtggcaaatc ctaacctttt atatttctct acaggggcgc ggcgtgggga
     4261 caattcaacg cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag cgaggagccg
     4321 taatttttgc ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga ttatacatgg
     4381 ggatgtatgg gctaaatgta cgggcgacag tcacatcatg cccctgagct gcgcacgtca
     4441 agactgtcaa ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc cggcggggac
     4501 gaggcaagct aaacagatct ggcgcgcctt aattaacccg gggatccaca ggacgggtgt
     4561 ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg
     4621 gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc
     4681 atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg
     4741 caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt
     4801 gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca
     4861 atttaactgt gataaactac cgcattaaag ctttttcttt ccaatttttt ttttttcgtc
     4921 attatagaaa tcattacgac cgagattccc gggtaataac tgatataatt aaattgaagc
     4981 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     5041 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     5101 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     5161 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     5221 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     5281 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     5341 atattctcca gtagctaggg agcccttgca tgacaattct gctaacatca aaaggcctct
     5401 aggttccttt gttacttctt ccgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     5461 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     5521 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     5581 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     5641 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     5701 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     5761 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     5821 agctttcgac atgatttatc ttcgtttcct gcaggttttt gttctgtgca gttgggttaa
     5881 gaatactggg caatttcatg tttcttcaac accacatatg cgtatatata ccaatctaag
     5941 tctgtgctcc ttccttcgtt cttccttctg ctcggagatt accgaatcaa aaaaatttca
     6001 aagaaaccgg aatcaaaaaa aagaacaaaa aaaaaaaaga tgaattgaaa agct
//
