LOCUS       40924_25771        6285 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast selection vector pIS418, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Sadowski I, Lourenco P, Parent J.
  TITLE     Dominant marker vectors for selecting yeast mating products
  JOURNAL   Yeast 25 (8), 595-599 (2008)
  PUBMED    18613257
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Sadowski I, Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2008) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 6285)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6285)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "2008"; volume: "25"; issue: "8"; pages: "595-599"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-MAR-2008) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..845
                     /label=ARS1/trp1
                     /note="ARS1/trp1"
     promoter        complement(881..899)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1245..1349
                     /label=AmpR promoter
     CDS             1350..2207
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2381..2969
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3227..3245
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            complement(3350..4706)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     CDS             complement(5263..6063)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLATGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6064..6280)
                     /label=URA3 promoter
     rep_origin      6282..6285
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 tacattttat gttagctggt ggactgacgc cagaaaatgt tggtgatgcg cttagattaa
       61 atggcgttat tggtgttgat gtaagcggag gtgtggagac aaatggtgta aaagactcta
      121 acaaaatagc aaatttcgtc aaaaatgcta agaaataggt tattactgag tagtatttat
      181 ttaagtattg tttgtgcact tgcctgcagg ccttttgaaa agcaagcata aaagatctaa
      241 acataaaatc tgtaaaataa caagatgtaa agataatgct aaatcatttg gctttttgat
      301 tgattgtaca ggaaaatata catcgcaggg ggttgacttt taccatttca ccgcaatgga
      361 atcaaacttg ttgaagagaa tgttcacagg cgcatacgct acaatgaccc gattcttgct
      421 agccttttct cggtcttgca aacaaccgcc ggcagcttag tatataaata cacatgtaca
      481 tacctctctc cgtatcctcg taatcatttt cttgtattta tcgtcttttc gctgtaaaaa
      541 ctttatcaca cttatctcaa atacacttat taaccgcttt tactattatc ttctacgctg
      601 acagtaatat caaacagtga cacatattaa acacagtggt ttctttgcat aaacaccatc
      661 agcctcaagt cgtcaagtaa agatttcgtg ttcatgcaga tagataacaa tctatatgtt
      721 gataattagc gttgcctcat caatgcgaga tccgtttaac cggaccctag tgcacttacc
      781 ccacgttcgg tccactgtgt gccgaacatg ctccttcact attttaacat gtgggaattc
      841 ctcgacctgc agcgtacgaa gcttcagctg gcggccgcgt tctatagtgt cacctaaatc
      901 gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt
      961 ccatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     1021 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     1081 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     1141 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     1201 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     1441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     1501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     1561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     1621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     1681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     1741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     1801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     1861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     1921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     1981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2521 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2761 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     3181 cgcgttggcc gattcattaa tgcaggttaa cctggcttat cgaaattaat acgactcact
     3241 atagggagac cggcagatcc gcggccgcat aggccactag tggatctgat atcatcgatg
     3301 aattcgagct cgtttaaact ggatggcggc gttagtatcg aatcgacagc agtatagcga
     3361 ccagcattca catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg
     3421 ggcagatgat gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa
     3481 caactacaat ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat
     3541 tgtcagtact gattagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     3601 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     3661 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     3721 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     3781 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     3841 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     3901 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ctgttaaaag gacaattaca
     3961 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     4021 tgaatcagga tattcttcta atacctggaa tgctgttttg ccggggatcg cagtggtgag
     4081 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     4141 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     4201 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     4261 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     4321 atttaatcgc ggcctcgaaa cgtgagtctt ttccttaccc atggttgttt atgttcggat
     4381 gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa gtatatgaaa gaagaacctc
     4441 agtggcaaat cctaaccttt tatatttctc tacaggggcg cggcgtgggg acaattcaac
     4501 gcgtctgtga ggggagcgtt tccctgctcg caggtctgca gcgaggagcc gtaatttttg
     4561 cttcgcgccg tgcggccatc aaaatgtatg gatgcaaatg attatacatg gggatgtatg
     4621 ggctaaatgt acgggcgaca gtcacatcat gcccctgagc tgcgcacgtc aagactgtca
     4681 aggagggtat tctgggcctc catgtcgctg gccgggtgac ccggcgggga cgaggcaagc
     4741 taaacagatc tggcgcgcct taattaaccc ggggatccac aggacgggtg tggtcgccat
     4801 gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa
     4861 gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc
     4921 tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc
     4981 cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat
     5041 gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg
     5101 tgataaacta ccgcattaaa gctttttctt tccaattttt tttttttcgt cattatagaa
     5161 atcattacga ccgagattcc cgggtaataa ctgatataat taaattgaag ctctaatttg
     5221 tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct ggccgcatct
     5281 tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct tagcatccct
     5341 tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag agaccacatc
     5401 atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac ccacaccggg
     5461 tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt gagcaataaa
     5521 gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag tatattctcc
     5581 agtagctagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc taggttcctt
     5641 tgttacttct tccgccgcct gcttcaaacc gctaacaata cctgggccca ccacaccgtg
     5701 tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt actgcaattt
     5761 gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat tgtacttggc
     5821 ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca agatatccac
     5881 atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca gtaattcctt
     5941 ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca tgatattaaa
     6001 tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg tagctttcga
     6061 catgatttat cttcgtttcc tgcaggtttt tgttctgtgc agttgggtta agaatactgg
     6121 gcaatttcat gtttcttcaa caccacatat gcgtatatat accaatctaa gtctgtgctc
     6181 cttccttcgt tcttccttct gctcggagat taccgaatca aaaaaatttc aaagaaaccg
     6241 gaatcaaaaa aaagaacaaa aaaaaaaaag atgaattgaa aagct
//
