LOCUS       40924_25766        4886 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS385, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4886)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4886)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..939
                     /label=3' flanking lys2 YIP-out fragment
                     /note="3' flanking lys2 YIP-out fragment"
     CDS             complement(1342..2142)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2143..2358)
                     /label=URA3 promoter
     promoter        2456..2560
                     /label=AmpR promoter
     CDS             2561..3418
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3592..4180
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4417..4886
                     /label=5' flanking lys2 YIP-in fragment
                     /note="5' flanking lys2 YIP-in fragment"
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attccagcct ctgcaagcgg gctcactggt ggatatggtc
      121 agtccaaatg ggctgctgag tacatcatta gacgtgcagg tgaaaggggc ctacgtgggt
      181 gtattgtcag accaggttac gtaacaggtg cctctgccaa tggttcttca aacacagatg
      241 atttcttatt gagatttttg aaaggttcag tccaattagg taagattcca gatatcgaaa
      301 attccgtgaa tatggttcca gtagatcatg ttgctcgtgt tgttgttgct acgtctttga
      361 atcctcccaa agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca agaatattat
      421 tcaaagacta cttgtatact ttacacgatt atggttacga tgtcgaaatc gaaagctatt
      481 ctaaatggaa gaaatcattg gaggcgtctg ttattgacag gaatgaagaa aatgcgttgt
      541 atcctttgct acacatggtc ttagacaact tacctgaaag taccaaagct ccggaactag
      601 acgataggaa cgccgtggca tctttaaaga aagacaccgc atggacaggt gttgattggt
      661 ctaatggaat aggtgttact ccagaagagg ttggtatata tattgcattt ttaaacaagg
      721 ttggattttt acctccacca actcataatg acaaacttcc actgccaagt atagaactaa
      781 ctcaagcgca aataagtcta gttgcttcag gtgctggtgc tcgtggaagc tccgcagcag
      841 cttaaggttg agcattacgt atgatatgtc catgtacaat aattaaatat gaattaggag
      901 aaagacttag cttcttttcg ggtgatgtca cttctcgacg cctggcgtaa tagcgaagag
      961 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg
     1021 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt
     1081 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     1141 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     1201 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     1261 gggtaataac tgatataatt aaattgaagc tctaatttgt gagtttagta tacatgcatt
     1321 tacttataat acagtttttt agttttgctg gccgcatctt ctcaaatatg cttcccagcc
     1381 tgcttttctg taacgttcac cctctacctt agcatccctt ccctttgcaa atagtcctct
     1441 tccaacaata ataatgtcag atcctgtaga gaccacatca tccacggttc tatactgttg
     1501 acccaatgcg tctcccttgt catctaaacc cacaccgggt gtcataatca accaatcgta
     1561 accttcatct cttccaccca tgtctctttg agcaataaag ccgataacaa aatctttgtc
     1621 gctcttcgca atgtcaacag tacccttagt atattctcca gtagataggg agcccttgca
     1681 tgacaattct gctaacatca aaaggcctct aggttccttt gttacttctt ctgccgcctg
     1741 cttcaaaccg ctaacaatac ctgggcccac cacaccgtgt gcattcgtaa tgtctgccca
     1801 ttctgctatt ctgtatacac ccgcagagta ctgcaatttg actgtattac caatgtcagc
     1861 aaattttctg tcttcgaaga gtaaaaaatt gtacttggcg gataatgcct ttagcggctt
     1921 aactgtgccc tccatggaaa aatcagtcaa gatatccaca tgtgttttta gtaaacaaat
     1981 tttgggacct aatgcttcaa ctaactccag taattccttg gtggtacgaa catccaatga
     2041 agcacacaag tttgtttgct tttcgtgcat gatattaaat agcttggcag caacaggact
     2101 aggatgagta gcagcacgtt ccttatatgt agctttcgac atgatttatc ttcgtttcct
     2161 gcatgttttt gttctgtgca gttgggttaa gaatactggg caatttcatg tttcttcaac
     2221 actacatatg cgtatatata ccaatctaag tctgtgctcc ttccttcgtt cttccttctg
     2281 ttcggagatt accgaatcaa aaaaatttca aagaaaccga aatcaaaaaa aagaataaaa
     2341 aaaaaatgat gaattgaatt gaaaagctgg cctcgtgata cgcctatttt tataggttaa
     2401 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     2461 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2521 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     2581 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     2641 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     2701 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     2761 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     2821 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     2881 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     2941 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     3001 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     3061 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     3121 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     3181 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     3241 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     3301 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     3361 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     3421 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     3481 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     3541 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     3601 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     3661 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     3721 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     3781 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     3841 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     3901 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     3961 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     4021 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     4081 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     4141 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     4201 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     4261 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     4321 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     4381 gcctctcccc gcgcgttggc cgattcatta atgcaggcag atcctctctc tgatcaccgc
     4441 atcatccaag gatagtttac ctgatccaac taagaacttg ggctggtgcg atttcgtggg
     4501 gtgtattcac gacattttcc aggacaatgc tgaagccttc ccagagagaa cctgtgttgt
     4561 ggagactcca acactaaatt ccgacaagtc ccgttctttc acttatcgcg acatcaaccg
     4621 cacttctaac atagttgccc attatttgat taaaacaggt atcaaaagag gtgatgtagt
     4681 gatgatctat tcttctaggg gtgtggattt gatggtatgt gtgatgggtg tcttgaaagc
     4741 cggcgcaacc ttttcagtta tcgaccctgc atatccccca gccagacaaa ccatttactt
     4801 aggtgttgct aaaccacgtg ggttgattgt tattagagct gctggacaat tggatcaact
     4861 agtagaagat tacatcaatg atgctg
//
