LOCUS       40924_25761        4866 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS376, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4866)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4866)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..958
                     /label=3' flanking leu2 YIP-out fragment
                     /note="3' flanking leu2 YIP-out fragment"
     CDS             complement(1366..2166)
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(2167..2382)
                     /label=URA3 promoter
     promoter        2480..2584
                     /label=AmpR promoter
     CDS             2585..3442
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3616..4204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4441..4866
                     /label=5' flanking leu2 YIP-in fragment
                     /note="5' flanking leu2 YIP-in fragment"
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attccaggca tcaattgatt gattctgccg ccatgatcct
      121 agttaagaac ccaacccacc taaatggtat tataatcacc agcaacatgt ttggtgatat
      181 catctccgat gaagcctccg ttatcccagg ttccttgggt ttgttgccat ctgcgtcctt
      241 ggcctctttg ccagacaaga acaccgcatt tggtttgtac gaaccatgcc acggttctgc
      301 tccagatttg ccaaagaata aggtcaaccc tatcgccact atcttgtctg ctgcaatgat
      361 gttgaaattg tcattgaact tgcctgaaga aggtaaggcc attgaagatg cagttaaaaa
      421 ggttttggat gcaggtatca gaactggtga tttaggtggt tccaacagta ccacggaagt
      481 cggtgatgct gtcgccgaag aagttaagaa aatccttgct taaaaagatt ctcttttttt
      541 atgatatttg tacataaact ttataaatga aattcataat agaaacgaca cgaaattaca
      601 aaatggaata tgttcatagg gtagacgaaa ctatatacgc aatctacata catttatcaa
      661 gaaggagaaa aaggaggatg taaaggaata caggtaagca aattgatact aatggctcaa
      721 cgtgataagg aaaaagaatt gcactttaac attaatattg acaaggagga gggcaccaca
      781 caaaaagtta ggtgtaacag aaaatcatga aactatgatt cctaatttat atattggagg
      841 attttctcta aaaaaaaaaa aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg
      901 atggagttta agtcaatacc ttcttgaacc atttcccata atggtgaaag ttccctcctc
      961 gacgcctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1021 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1081 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     1141 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     1201 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     1261 ccgaaacgcg cgagacgaaa gggcgggtaa taactgatat aattaaattg aagctctaat
     1321 ttgtgagttt agtatacatg catttactta taatacagtt ttttagtttt gctggccgca
     1381 tcttctcaaa tatgcttccc agcctgcttt tctgtaacgt tcaccctcta ccttagcatc
     1441 ccttcccttt gcaaatagtc ctcttccaac aataataatg tcagatcctg tagagaccac
     1501 atcatccacg gttctatact gttgacccaa tgcgtctccc ttgtcatcta aacccacacc
     1561 gggtgtcata atcaaccaat cgtaaccttc atctcttcca cccatgtctc tttgagcaat
     1621 aaagccgata acaaaatctt tgtcgctctt cgcaatgtca acagtaccct tagtatattc
     1681 tccagtagat agggagccct tgcatgacaa ttctgctaac atcaaaaggc ctctaggttc
     1741 ctttgttact tcttctgccg cctgcttcaa accgctaaca atacctgggc ccaccacacc
     1801 gtgtgcattc gtaatgtctg cccattctgc tattctgtat acacccgcag agtactgcaa
     1861 tttgactgta ttaccaatgt cagcaaattt tctgtcttcg aagagtaaaa aattgtactt
     1921 ggcggataat gcctttagcg gcttaactgt gccctccatg gaaaaatcag tcaagatatc
     1981 cacatgtgtt tttagtaaac aaattttggg acctaatgct tcaactaact ccagtaattc
     2041 cttggtggta cgaacatcca atgaagcaca caagtttgtt tgcttttcgt gcatgatatt
     2101 aaatagcttg gcagcaacag gactaggatg agtagcagca cgttccttat atgtagcttt
     2161 cgacatgatt tatcttcgtt tcctgcatgt ttttgttctg tgcagttggg ttaagaatac
     2221 tgggcaattt catgtttctt caacactaca tatgcgtata tataccaatc taagtctgtg
     2281 ctccttcctt cgttcttcct tctgttcgga gattaccgaa tcaaaaaaat ttcaaagaaa
     2341 ccgaaatcaa aaaaaagaat aaaaaaaaaa tgatgaattg aattgaaaag ctggcctcgt
     2401 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     2461 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     2521 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     2581 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     2641 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     2701 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     2761 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     2821 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     2881 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     2941 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3001 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3061 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3121 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     3181 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     3241 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     3301 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     3361 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     3421 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     3481 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     3541 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     3601 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     3661 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     3721 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     3781 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     3841 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     3901 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     3961 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     4021 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     4081 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     4141 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     4201 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     4261 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     4321 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     4381 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     4441 gcagatccta gtcatctaaa ttagtggaag ctgaaacgca aggattgata atgtaatagg
     4501 atcaatgaat attaacatat aaaatgatga taataatatt tatagaattg tgtagaattg
     4561 cagattccct tttatggatt cctaaatcct cgactcctcg gcgcgccgag gagtcgagga
     4621 gaacttctag tatatctaca tacctaatat tattgcctta ttaaaaatgg aatcccaaca
     4681 attacatcaa aatccacatt ctcttcaaaa tcaattgtcc tgtacttcct tgttcatgtg
     4741 tgttcaaaaa cgttatattt ataggataat tatactctat ttctcaacaa gtaattggtt
     4801 gtttggccga gcggtctaag gcgcctgatt caagaaatat cttgaccgca gttaactgtg
     4861 ggcctg
//
