LOCUS       40924_25756        4950 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Integration vector pIS375, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Disintegrator vectors for single copy yeast chromosomal integration
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Sadowski I, Su T-C., Parent J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2006) Biochemistry and Molecular Biology, 
            University of British Columbia, 2350 Health Sciences Mall, 
            Vancouver, BC V6T 1Z3, Canada
REFERENCE   3  (bases 1 to 4950)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4950)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-DEC-2006) Biochemistry and Molecular Biology, University of 
            British Columbia, 2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, 
            Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4950
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..84
                     /label=polylinker cloning sequence
                     /note="polylinker cloning sequence"
     misc_feature    85..686
                     /label=3' flanking met15 YIP-in fragment
                     /note="3' flanking met15 YIP-in fragment"
     rep_origin      complement(926..1514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1688..2545)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2546..2650)
                     /label=AmpR promoter
     promoter        2748..2963
                     /label=URA3 promoter
     CDS             2964..3764
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        4600..4946
                     /label=MET17 promoter
                     /note="expression is repressed by methionine"
ORIGIN
        1 gcggccgcct cgagagatct aagcttatcg atgcatgcct gcaggtcgac tctagaggat
       61 ccccggggta ccgagctcga attccagggt tggtgatgcc aagaccttag tcattgctcc
      121 atacttcact acccacaaac aattaaatga caaagaaaag ttggcatctg gtgttaccaa
      181 ggacttaatt cgtgtctctg ttggtatcga atttattgat gacattattg cagacttcca
      241 gcaatctttt gaaactgttt tcgctggcca aaaaccatga gtgtgcgtaa tgagttgtaa
      301 aattatgtat aaacctactt tctctcacaa gtactatact tttataaaac gaactttatt
      361 gaaatgaata tccttttttt cccttgttac atgtcgtgac tcgtactttg aacctaaatt
      421 gttctaacat caaagaacag tgttaattcg cagtcgagaa gaaaaatatg gtgaacaaga
      481 ctcatctact tcatgagact actttacgcc tcctataaag ctgtcacact ggataaattt
      541 attgtaggac caagttacaa aagaggatga tggaggtttc tttacaataa agaagcacat
      601 gtgtgttaac gtttttagta tttgcttgtt atgtaaatca ggaaaacttc gcgggatttg
      661 gttggatgct actttccata cggatctgcc tgcattaatg aatcggccaa cgcgcgggga
      721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     1381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     2701 tgacattaac ctataaaaat aggcgtatca cgaggccagc ttttcaattc aattcatcat
     2761 ttttttttta ttcttttttt tgatttcggt ttctttgaaa tttttttgat tcggtaatct
     2821 ccgaacagaa ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat
     2881 gtagtgttga agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa
     2941 catgcaggaa acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc
     3001 atcctagtcc tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt
     3061 gtgcttcatt ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc
     3121 ccaaaatttg tttactaaaa acacatgtgg atatcttgac tgatttttcc atggagggca
     3181 cagttaagcc gctaaaggca ttatccgcca agtacaattt tttactcttc gaagacagaa
     3241 aatttgctga cattggtaat acagtcaaat tgcagtactc tgcgggtgta tacagaatag
     3301 cagaatgggc agacattacg aatgcacacg gtgtggtggg cccaggtatt gttagcggtt
     3361 tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg ccttttgatg ttagcagaat
     3421 tgtcatgcaa gggctcccta tctactggag aatatactaa gggtactgtt gacattgcga
     3481 agagcgacaa agattttgtt atcggcttta ttgctcaaag agacatgggt ggaagagatg
     3541 aaggttacga ttggttgatt atgacacccg gtgtgggttt agatgacaag ggagacgcat
     3601 tgggtcaaca gtatagaacc gtggatgatg tggtctctac aggatctgac attattattg
     3661 ttggaagagg actatttgca aagggaaggg atgctaaggt agagggtgaa cgttacagaa
     3721 aagcaggctg ggaagcatat ttgagaagat gcggccagca aaactaaaaa actgtattat
     3781 aagtaaatgc atgtatacta aactcacaaa ttagagcttc aatttaatta tatcagttat
     3841 tacccgccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     3901 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     3961 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     4021 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     4081 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     4141 cgggcctctt cgctattacg ccaggcgtcg aggatttagg aatccataaa agggaatctg
     4201 caattctaca caattctata aatattatta tcatcgtttt atatgttaat attcattgat
     4261 cctattacat tatcaatcct tgcgtttcag cttccactaa tttagatgac tatttctcat
     4321 catttgcgtc atcttctaac accgtatatg ataatatact agtaacgtaa atactagtta
     4381 gtagatgata gttgattttt attccaacac taagaaataa tttcgccatt tcttgaatgt
     4441 atttaaagat atttaatgct ataatagaca tttaaatcca attcttccaa catacaatgg
     4501 gagtttggcc gagtggttta aggcgtcaga tttaggtgga tttaacctct aaaatctctg
     4561 atatcttcgg atgcaagggt tcgaatccct tagctctcat tattttttgc tttttctctt
     4621 gaggtcacat gatcgcaaaa tggcaaatgg cacgtgaagc tgtcgatatt ggggaactgt
     4681 ggtggttggc aaatgactaa ttaagttagt caaggcgcca tcctcatgaa aactgtgtaa
     4741 cataataacc gaagtgtcga aaaggtggca ccttgtccaa ttgaacacgc tcgatgaaaa
     4801 aaataagata tatataaggt taagtaaagc gtctgttaga aaggaagttt ttcctttttc
     4861 ttgctctctt gtcttttcat ctactatttc cttcgtgtaa tacagggtcg tcagatacat
     4921 agatacaatt ctattacccc catccacctg
//
